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- W2945651569 abstract "For the analysis of the subcellular distribution of Ca ion in human platelet, the washed platelet suspension was fixed in the potassium antimonate-OsO4 method or in the potassium oxalate-glutaraldehyde method without post-fixation with OSO4. The STEM-images were observed with scanning image system fitted with the transmission electron microscope without electron staining. The dense deposition of antimonate or oxalate was found in plasmalemma, the membrane of open canalicular system, mitochondria, α-granules and dense bodies. It was revealed with the energy dispersive type electron probe x-ray microanalyzer system that the deposition of oxalate was exclusively composed of Ca. In the process of platelet aggregation with various agents, α-granules and dense bodies were expelled out together with Ca, which suggested the least role of these ion depositions in the aggregation and clot retraction. The translocation of antimonate deposits was not observed in the Verapamil-treated platelets even after the addition of aggregation agents. This phenomenon indicated that this drug inhibited the release of Ca from the Ca-storing organelles. The distribution of Ca was identical in the platelets of a case of thrombasthenia and the translocation of Ca was not observed with the addition of various aggregating agents." @default.
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- W2945651569 date "1979-01-01" @default.
- W2945651569 modified "2023-09-23" @default.
- W2945651569 title "Electron Microscopic Analysis of Subcellular Distribution and Translocation of Ca ION in Human Platelet" @default.
- W2945651569 doi "https://doi.org/10.1055/s-0039-1684465" @default.
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