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- W2945773152 abstract "Fluorescence in situ hybridization (FISH) reveals the abundance and positioning of nucleic acid sequences in fixed samples. Despite recent advances in multiplexed amplification of FISH signals, it remains challenging to achieve high levels of simultaneous amplification and sequential detection with high sampling efficiency and simple workflows. Here we introduce signal amplification by exchange reaction (SABER), which endows oligonucleotide-based FISH probes with long, single-stranded DNA concatemers that aggregate a multitude of short complementary fluorescent imager strands. We show that SABER amplified RNA and DNA FISH signals (5- to 450-fold) in fixed cells and tissues. We also applied 17 orthogonal amplifiers against chromosomal targets simultaneously and detected mRNAs with high efficiency. We then used 10-plex SABER-FISH to identify in vivo introduced enhancers with cell-type-specific activity in the mouse retina. SABER represents a simple and versatile molecular toolkit for rapid and cost-effective multiplexed imaging of nucleic acid targets. Using primer-exchange reactions, SABER extends FISH probes with repetitive sequences that can accommodate multiple fluorescent imager strands, resulting in up to 450-fold signal amplification. SABER is showcased in DNA and RNA FISH experiments across a range of complex biological samples." @default.
- W2945773152 created "2019-05-29" @default.
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- W2945773152 date "2019-05-20" @default.
- W2945773152 modified "2023-10-01" @default.
- W2945773152 title "SABER amplifies FISH: enhanced multiplexed imaging of RNA and DNA in cells and tissues" @default.
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- W2945773152 doi "https://doi.org/10.1038/s41592-019-0404-0" @default.
- W2945773152 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6544483" @default.
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- W2945773152 hasPublicationYear "2019" @default.
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