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- W2945841034 abstract "Fibrinopeptide-A (FpA) is thought to be the most sensitive parameter to indicate hypercoagulability with increased fibrin formation in man. Previously described radioimmunoassays for FpA are very time consuming. We present therefore a modification of the assay, which is less time consuming, sensitive, reproducable and reliable. The following modifications were made on the original method: 1. plasma dialysis was omitted 2. performance on microtiter-plates 3. double antibody method. The sensitivity was improved to 0.16 ng FpA / ml plasma. The mean concentration of FpA in dialysed and undialysed plasma did not differ significantly (p<0.001). Effective separation of fibrinogen was achieved by aethanol extraction alone. The mean concentration of FpA correlated highly, when the tracer was separated by charcoal or second antibody (r = 0.96). The recovery was improved to 85%. In healthy persons 0.16-2.5 ng FpA/ml plasma were measured. In patients with venous thrombosis or myocardial infarction in the history FpA was elevated significantly to 2.0 ng/ml-19.6 ng/ml, indicating that the FpA is a usefull tool in diagnosis of hypercoagulability." @default.
- W2945841034 created "2019-05-29" @default.
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- W2945841034 date "1979-07-01" @default.
- W2945841034 modified "2023-09-23" @default.
- W2945841034 title "A New Radioimmunoassay-Technique For Fibrinopeptide-A In Human Plasma" @default.
- W2945841034 doi "https://doi.org/10.1055/s-0038-1665772" @default.
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