Matches in SemOpenAlex for { <https://semopenalex.org/work/W2945844579> ?p ?o ?g. }
Showing items 1 to 100 of
100
with 100 items per page.
- W2945844579 endingPage "758" @default.
- W2945844579 startingPage "753" @default.
- W2945844579 abstract "Protein refolding is currently a fundamental problem in biophysics and molecular biology. We have studied the refolding process of frutalin, a tetrameric lectin that presents structural homology with jacalin but shows a more marked biological activity. The initial state in our refolding puzzle was that proteins were unfolded after thermal denaturation or denaturation induced by guanidine hydrochloride, and under both conditions, frutalin was refolded. The denaturation curves, measured by fluorescence emission, gave values of conformational stability of 17.12 kJ x mol(-1) and 12.34 kJ x mol(-1), in the presence and absence of d-galactose, respectively. Native, unfolded, refolded frutalin and a distinct molecular form denoted misfolded, were separated by size-exclusion chromatography (SEC) on Superdex 75. The native and unfolded samples together with the fractions separated by SEC were also analyzed for heamagglutination activity by CD and fluorescence spectroscopy. The secondary structure content of refolded frutalin estimated from the CD spectra was found to be close to that of the native molecule. All the results obtained confirmed the successful refolding of the protein and suggested a nucleation-condensation mechanism, whereby the sugar-binding site acts as a nucleus to initiate the refolding process. The refolded monomers, after adopting their native three-dimensional structures, spontaneously assemble to form tetramers." @default.
- W2945844579 created "2019-05-29" @default.
- W2945844579 creator A5025589081 @default.
- W2945844579 creator A5038626432 @default.
- W2945844579 creator A5041457526 @default.
- W2945844579 creator A5065721595 @default.
- W2945844579 date "2002-02-01" @default.
- W2945844579 modified "2023-10-17" @default.
- W2945844579 title "Unfolding and refolding studies of frutalin, a tetrameric<scp>d</scp>-galactose binding lectin" @default.
- W2945844579 cites W1606562654 @default.
- W2945844579 cites W1821925018 @default.
- W2945844579 cites W1997877357 @default.
- W2945844579 cites W2009929688 @default.
- W2945844579 cites W2014095962 @default.
- W2945844579 cites W2016607365 @default.
- W2945844579 cites W2018059128 @default.
- W2945844579 cites W2025545917 @default.
- W2945844579 cites W2032363114 @default.
- W2945844579 cites W2034822406 @default.
- W2945844579 cites W2039894092 @default.
- W2945844579 cites W2048966080 @default.
- W2945844579 cites W2055800251 @default.
- W2945844579 cites W2075954862 @default.
- W2945844579 cites W2079884830 @default.
- W2945844579 cites W2116076986 @default.
- W2945844579 cites W2158738787 @default.
- W2945844579 cites W2163895770 @default.
- W2945844579 cites W2171755502 @default.
- W2945844579 cites W4240929891 @default.
- W2945844579 cites W4293247451 @default.
- W2945844579 doi "https://doi.org/10.1046/j.0014-2956.2002.02742.x" @default.
- W2945844579 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/11846776" @default.
- W2945844579 hasPublicationYear "2002" @default.
- W2945844579 type Work @default.
- W2945844579 sameAs 2945844579 @default.
- W2945844579 citedByCount "18" @default.
- W2945844579 countsByYear W29458445792014 @default.
- W2945844579 countsByYear W29458445792015 @default.
- W2945844579 countsByYear W29458445792017 @default.
- W2945844579 crossrefType "journal-article" @default.
- W2945844579 hasAuthorship W2945844579A5025589081 @default.
- W2945844579 hasAuthorship W2945844579A5038626432 @default.
- W2945844579 hasAuthorship W2945844579A5041457526 @default.
- W2945844579 hasAuthorship W2945844579A5065721595 @default.
- W2945844579 hasConcept C12554922 @default.
- W2945844579 hasConcept C133571119 @default.
- W2945844579 hasConcept C13965031 @default.
- W2945844579 hasConcept C181199279 @default.
- W2945844579 hasConcept C185592680 @default.
- W2945844579 hasConcept C193363816 @default.
- W2945844579 hasConcept C19472624 @default.
- W2945844579 hasConcept C19869320 @default.
- W2945844579 hasConcept C202751555 @default.
- W2945844579 hasConcept C204328495 @default.
- W2945844579 hasConcept C2776923230 @default.
- W2945844579 hasConcept C2779463914 @default.
- W2945844579 hasConcept C2780758401 @default.
- W2945844579 hasConcept C55493867 @default.
- W2945844579 hasConcept C62614982 @default.
- W2945844579 hasConcept C8010536 @default.
- W2945844579 hasConcept C86803240 @default.
- W2945844579 hasConceptScore W2945844579C12554922 @default.
- W2945844579 hasConceptScore W2945844579C133571119 @default.
- W2945844579 hasConceptScore W2945844579C13965031 @default.
- W2945844579 hasConceptScore W2945844579C181199279 @default.
- W2945844579 hasConceptScore W2945844579C185592680 @default.
- W2945844579 hasConceptScore W2945844579C193363816 @default.
- W2945844579 hasConceptScore W2945844579C19472624 @default.
- W2945844579 hasConceptScore W2945844579C19869320 @default.
- W2945844579 hasConceptScore W2945844579C202751555 @default.
- W2945844579 hasConceptScore W2945844579C204328495 @default.
- W2945844579 hasConceptScore W2945844579C2776923230 @default.
- W2945844579 hasConceptScore W2945844579C2779463914 @default.
- W2945844579 hasConceptScore W2945844579C2780758401 @default.
- W2945844579 hasConceptScore W2945844579C55493867 @default.
- W2945844579 hasConceptScore W2945844579C62614982 @default.
- W2945844579 hasConceptScore W2945844579C8010536 @default.
- W2945844579 hasConceptScore W2945844579C86803240 @default.
- W2945844579 hasIssue "3" @default.
- W2945844579 hasLocation W29458445791 @default.
- W2945844579 hasLocation W29458445792 @default.
- W2945844579 hasOpenAccess W2945844579 @default.
- W2945844579 hasPrimaryLocation W29458445791 @default.
- W2945844579 hasRelatedWork W1971875940 @default.
- W2945844579 hasRelatedWork W1981966805 @default.
- W2945844579 hasRelatedWork W1990659752 @default.
- W2945844579 hasRelatedWork W2022611611 @default.
- W2945844579 hasRelatedWork W2029873267 @default.
- W2945844579 hasRelatedWork W2139575802 @default.
- W2945844579 hasRelatedWork W2171414493 @default.
- W2945844579 hasRelatedWork W2413925646 @default.
- W2945844579 hasRelatedWork W2614337676 @default.
- W2945844579 hasRelatedWork W3134297621 @default.
- W2945844579 hasVolume "269" @default.
- W2945844579 isParatext "false" @default.
- W2945844579 isRetracted "false" @default.
- W2945844579 magId "2945844579" @default.
- W2945844579 workType "article" @default.