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- W2945921566 endingPage "2168" @default.
- W2945921566 startingPage "2150" @default.
- W2945921566 abstract "Dividing cells reorganize their microtubule cytoskeleton into a bipolar spindle, which moves one set of sister chromatids to each nascent daughter cell. Early spindle assembly models postulated that spindle pole–derived microtubules search the cytoplasmic space until they randomly encounter a kinetochore to form a stable attachment. More recent work uncovered several additional, centrosome-independent microtubule generation pathways, but the contributions of each pathway to spindle assembly have remained unclear. Here, we combined live microscopy and mathematical modeling to show that most microtubules nucleate at noncentrosomal regions in dividing human cells. Using a live-cell probe that selectively labels aged microtubule lattices, we demonstrate that the distribution of growing microtubule plus ends can be almost entirely explained by Augmin-dependent amplification of long-lived microtubule lattices. By ultrafast 3D lattice light-sheet microscopy, we observed that this mechanism results in a strong directional bias of microtubule growth toward individual kinetochores. Our systematic quantification of spindle dynamics reveals highly coordinated microtubule growth during kinetochore fiber assembly." @default.
- W2945921566 created "2019-05-29" @default.
- W2945921566 creator A5015070517 @default.
- W2945921566 creator A5037958980 @default.
- W2945921566 creator A5041759383 @default.
- W2945921566 creator A5048568518 @default.
- W2945921566 creator A5067477836 @default.
- W2945921566 creator A5085508441 @default.
- W2945921566 date "2019-05-21" @default.
- W2945921566 modified "2023-09-29" @default.
- W2945921566 title "Augmin accumulation on long-lived microtubules drives amplification and kinetochore-directed growth" @default.
- W2945921566 cites W1606409542 @default.
- W2945921566 cites W1699156920 @default.
- W2945921566 cites W1933219102 @default.
- W2945921566 cites W1965404941 @default.
- W2945921566 cites W1970489775 @default.
- W2945921566 cites W1981294799 @default.
- W2945921566 cites W1985499212 @default.
- W2945921566 cites W1991690400 @default.
- W2945921566 cites W1996766186 @default.
- W2945921566 cites W1998263911 @default.
- W2945921566 cites W2000131947 @default.
- W2945921566 cites W2007557610 @default.
- W2945921566 cites W2014294449 @default.
- W2945921566 cites W2020487525 @default.
- W2945921566 cites W2023488271 @default.
- W2945921566 cites W2023695022 @default.
- W2945921566 cites W2028436548 @default.
- W2945921566 cites W2028511996 @default.
- W2945921566 cites W2031020964 @default.
- W2945921566 cites W2033062977 @default.
- W2945921566 cites W2033797646 @default.
- W2945921566 cites W2035050643 @default.
- W2945921566 cites W2038506832 @default.
- W2945921566 cites W2043410014 @default.
- W2945921566 cites W2048586937 @default.
- W2945921566 cites W2050293083 @default.
- W2945921566 cites W2051056477 @default.
- W2945921566 cites W2053270078 @default.
- W2945921566 cites W2058036222 @default.
- W2945921566 cites W2063766478 @default.
- W2945921566 cites W2064304660 @default.
- W2945921566 cites W2067518266 @default.
- W2945921566 cites W2068843702 @default.
- W2945921566 cites W2071817332 @default.
- W2945921566 cites W2083833358 @default.
- W2945921566 cites W2089393338 @default.
- W2945921566 cites W2097313305 @default.
- W2945921566 cites W2102199276 @default.
- W2945921566 cites W2102673682 @default.
- W2945921566 cites W2102960342 @default.
- W2945921566 cites W2105916176 @default.
- W2945921566 cites W2109200236 @default.
- W2945921566 cites W2111832125 @default.
- W2945921566 cites W2112378641 @default.
- W2945921566 cites W2113144687 @default.
- W2945921566 cites W2114918924 @default.
- W2945921566 cites W2117279934 @default.
- W2945921566 cites W2121696619 @default.
- W2945921566 cites W2126499761 @default.
- W2945921566 cites W2128377922 @default.
- W2945921566 cites W2129158700 @default.
- W2945921566 cites W2129798606 @default.
- W2945921566 cites W2131113110 @default.
- W2945921566 cites W2131282221 @default.
- W2945921566 cites W2131938193 @default.
- W2945921566 cites W2136880905 @default.
- W2945921566 cites W2149612795 @default.
- W2945921566 cites W2155923449 @default.
- W2945921566 cites W2157555261 @default.
- W2945921566 cites W2158133478 @default.
- W2945921566 cites W2159412636 @default.
- W2945921566 cites W2164962038 @default.
- W2945921566 cites W2167774038 @default.
- W2945921566 cites W2168495075 @default.
- W2945921566 cites W2169372688 @default.
- W2945921566 cites W2217038394 @default.
- W2945921566 cites W2345583633 @default.
- W2945921566 cites W2398631311 @default.
- W2945921566 cites W2465950095 @default.
- W2945921566 cites W2489294456 @default.
- W2945921566 cites W2529418719 @default.
- W2945921566 cites W2587723625 @default.
- W2945921566 cites W2605373831 @default.
- W2945921566 cites W2621053731 @default.
- W2945921566 cites W2750058181 @default.
- W2945921566 cites W2775650492 @default.
- W2945921566 cites W2782861198 @default.
- W2945921566 cites W2808597451 @default.
- W2945921566 cites W2950311991 @default.
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- W2945921566 doi "https://doi.org/10.1083/jcb.201805044" @default.
- W2945921566 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6605806" @default.
- W2945921566 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/31113824" @default.
- W2945921566 hasPublicationYear "2019" @default.
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