FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2946253886> ?p ?o ?g. }

• W2946253886 endingPage "10171" @default.
• W2946253886 startingPage "10160" @default.
• W2946253886 abstract "Microtubule-associated proteins (MAPs) regulate microtubule polymerization, dynamics, and organization. In addition, MAPs alter the motility of kinesin and dynein to control trafficking along microtubules. MAP7 (ensconsin, E-MAP-115) is a ubiquitous MAP that organizes the microtubule cytoskeleton in mitosis and neuronal branching. MAP7 also recruits kinesin-1 to microtubules. To understand how the activation of kinesin-1 by MAP7 regulates the motility of organelles transported by ensembles of kinesin and dynein, we isolated organelles and reconstituted their motility in vitro. In the absence of MAP7, isolated phagosomes exhibit approximately equal fractions of plus- and minus-end–directed motility along microtubules. MAP7 causes a pronounced shift in motility; phagosomes move toward the plus-end ∼80% of the time, and kinesin teams generate more force. To dissect MAP7-mediated regulation of kinesin-driven transport, we examined its effects on the motility and force generation of single and teams of full-length kinesin-1 motors. We find that MAP7 does not alter the force exerted by a single kinesin-1 motor, but instead increases its binding rate to the microtubule. For ensembles of kinesin, a greater number of kinesin motors are simultaneously engaged and generating force to preferentially target organelles toward the microtubule plus-end. Microtubule-associated proteins (MAPs) regulate microtubule polymerization, dynamics, and organization. In addition, MAPs alter the motility of kinesin and dynein to control trafficking along microtubules. MAP7 (ensconsin, E-MAP-115) is a ubiquitous MAP that organizes the microtubule cytoskeleton in mitosis and neuronal branching. MAP7 also recruits kinesin-1 to microtubules. To understand how the activation of kinesin-1 by MAP7 regulates the motility of organelles transported by ensembles of kinesin and dynein, we isolated organelles and reconstituted their motility in vitro. In the absence of MAP7, isolated phagosomes exhibit approximately equal fractions of plus- and minus-end–directed motility along microtubules. MAP7 causes a pronounced shift in motility; phagosomes move toward the plus-end ∼80% of the time, and kinesin teams generate more force. To dissect MAP7-mediated regulation of kinesin-driven transport, we examined its effects on the motility and force generation of single and teams of full-length kinesin-1 motors. We find that MAP7 does not alter the force exerted by a single kinesin-1 motor, but instead increases its binding rate to the microtubule. For ensembles of kinesin, a greater number of kinesin motors are simultaneously engaged and generating force to preferentially target organelles toward the microtubule plus-end." @default.
• W2946253886 created "2019-05-29" @default.
• W2946253886 creator A5020089804 @default.
• W2946253886 creator A5034153870 @default.
• W2946253886 creator A5035579449 @default.
• W2946253886 creator A5068846727 @default.
• W2946253886 creator A5072354474 @default.
• W2946253886 creator A5083895003 @default.
• W2946253886 date "2019-06-01" @default.
• W2946253886 modified "2023-10-18" @default.
• W2946253886 title "MAP7 regulates organelle transport by recruiting kinesin-1 to microtubules" @default.
• W2946253886 cites W1606977653 @default.
• W2946253886 cites W1898357466 @default.
• W2946253886 cites W1966086662 @default.
• W2946253886 cites W1967986618 @default.
• W2946253886 cites W1970866062 @default.
• W2946253886 cites W1972398314 @default.
• W2946253886 cites W1974345291 @default.
• W2946253886 cites W1981628382 @default.
• W2946253886 cites W1981662243 @default.
• W2946253886 cites W1989729787 @default.
• W2946253886 cites W1996764498 @default.
• W2946253886 cites W2001219990 @default.
• W2946253886 cites W2001969173 @default.
• W2946253886 cites W2019578272 @default.
• W2946253886 cites W2022214576 @default.
• W2946253886 cites W2026245994 @default.
• W2946253886 cites W2028375545 @default.
• W2946253886 cites W2031266198 @default.
• W2946253886 cites W2039348923 @default.
• W2946253886 cites W2046720103 @default.
• W2946253886 cites W2046748818 @default.
• W2946253886 cites W2056169188 @default.
• W2946253886 cites W2057096287 @default.
• W2946253886 cites W2078056153 @default.
• W2946253886 cites W2084112383 @default.
• W2946253886 cites W2085242832 @default.
• W2946253886 cites W2095674737 @default.
• W2946253886 cites W2096991927 @default.
• W2946253886 cites W2104583113 @default.
• W2946253886 cites W2121302141 @default.
• W2946253886 cites W2123272295 @default.
• W2946253886 cites W2140405694 @default.
• W2946253886 cites W2157256220 @default.
• W2946253886 cites W2239857015 @default.
• W2946253886 cites W2298933030 @default.
• W2946253886 cites W2313478411 @default.
• W2946253886 cites W2426475512 @default.
• W2946253886 cites W2539080302 @default.
• W2946253886 cites W2571350962 @default.
• W2946253886 cites W2607503492 @default.
• W2946253886 cites W2762659278 @default.
• W2946253886 cites W2763958640 @default.
• W2946253886 cites W2763995657 @default.
• W2946253886 cites W2771928043 @default.
• W2946253886 cites W2809660340 @default.
• W2946253886 cites W2888402606 @default.
• W2946253886 cites W2913674846 @default.
• W2946253886 cites W2928375588 @default.
• W2946253886 cites W2952702685 @default.
• W2946253886 cites W2953010568 @default.
• W2946253886 cites W2979946585 @default.
• W2946253886 doi "https://doi.org/10.1074/jbc.ra119.008052" @default.
• W2946253886 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6664170" @default.
• W2946253886 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/31085585" @default.
• W2946253886 hasPublicationYear "2019" @default.
• W2946253886 type Work @default.
• W2946253886 sameAs 2946253886 @default.
• W2946253886 citedByCount "40" @default.
• W2946253886 countsByYear W29462538862019 @default.
• W2946253886 countsByYear W29462538862020 @default.
• W2946253886 countsByYear W29462538862021 @default.
• W2946253886 countsByYear W29462538862022 @default.
• W2946253886 countsByYear W29462538862023 @default.
• W2946253886 crossrefType "journal-article" @default.
• W2946253886 hasAuthorship W2946253886A5020089804 @default.
• W2946253886 hasAuthorship W2946253886A5034153870 @default.
• W2946253886 hasAuthorship W2946253886A5035579449 @default.
• W2946253886 hasAuthorship W2946253886A5068846727 @default.
• W2946253886 hasAuthorship W2946253886A5072354474 @default.
• W2946253886 hasAuthorship W2946253886A5083895003 @default.
• W2946253886 hasBestOaLocation W29462538861 @default.
• W2946253886 hasConcept C10447061 @default.
• W2946253886 hasConcept C106987784 @default.
• W2946253886 hasConcept C142669718 @default.
• W2946253886 hasConcept C1491633281 @default.
• W2946253886 hasConcept C168240541 @default.
• W2946253886 hasConcept C20418707 @default.
• W2946253886 hasConcept C2909009945 @default.
• W2946253886 hasConcept C29537977 @default.
• W2946253886 hasConcept C55493867 @default.
• W2946253886 hasConcept C58207958 @default.
• W2946253886 hasConcept C59006786 @default.
• W2946253886 hasConcept C84425145 @default.
• W2946253886 hasConcept C86803240 @default.
• W2946253886 hasConcept C93126451 @default.
• W2946253886 hasConcept C93304396 @default.
• W2946253886 hasConcept C94921385 @default.

• next