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- W2946266843 abstract "Human prekallikrein (PK) is in plasma complexed to High Moleculair Weight Kininogen (HMWK). The formation of a complex of PK with HMWK was demonstrated by cross-immunoelectrophoresis. Purified PK alone did not migrate upon electrophoresis, however when mixed with HMWK or Fletcher plasma, PK migrated with the same mobility as PK present in normal plasma. Also addition of PK to HMWK resulted in a reduction of the electrophoretic mobility of HMWK on cross-immunoelectrophoresis using anti-HMWK. Fitzgerald plasma which is deficient in HMWK has a PK clotting activity of 26% but no detectable PK-antigen level. Reconstitution with HMWK yielded PK-antigen levels corresponding to the PK-clotting activity. Addition of HMWK to normal plasma did not increase the PK-antigen levels. PK was activated by purified human (β-XIIa (28, 000 mol.wt) and by bovine α-XIIa (80, 000 mol.wt). Activation by αXIIa but not by by β-XII was enhanced by the presence of HMWK and dextran sulphate. After complete activation by α-XIIa in the presence of HMWK kallikrein remained complexed to HMWK. The generation of kallikrein activity paralelled the extent of proteolytic cleavage. SDS-polyacrylamide gel electrophoresis in the presence of reducing agents showed that both α-XIIa and β-XIIa generated fragments with apparent mol.wt’s of 52,000, 42,000 and 37,000. These fragments are initially held together by disulfide bonds, a second relatively slow cleavage generates fragments with similar mol. wt’s but observed on non reduced SDS-gels. This cleavage is not accompanied by a decrease in kallikrein activity." @default.
- W2946266843 created "2019-05-29" @default.
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- W2946266843 date "1979-01-01" @default.
- W2946266843 modified "2023-09-23" @default.
- W2946266843 title "Mechanism of Activation of Human Plasma Prekallikrein" @default.
- W2946266843 doi "https://doi.org/10.1055/s-0039-1684816" @default.
- W2946266843 hasPublicationYear "1979" @default.
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