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• W2946436563 abstract "Abstract Subjects increasing sperm DNA fragmentation (sDF) during Density Gradient Centrifugation (DGC), a common sperm selection procedure in Assisted Reproduction Techniques (ARTs), experience a 50% lower probability of pregnancy. Hence, identification of these subjects is of clinical importance. Here, we investigated whether such subjects are identified with higher accuracy detecting DNA fragmentation in viable (viable sDF) instead of total spermatozoa (total sDF) and whether swim up, an alternative procedure to DGC, does not increase sDF. With DGC, we identified 10/20 subjects increasing total sDF, and 2 more subjects using viable sDF. With swim up, we identified 8/40 subjects increasing total sDF, and 8 more subjects using viable sDF. In addition, viable sDF reveals more accurately the increase of the damage when it occurs. Finally, a multivariate analysis demonstrated that the proportional increase of sDF was higher after DGC respect to swim up. In conclusion, viable sDF is a more accurate parameter to reveal the increase of the damage by selection both with swim up and DGC. Swim up increases sDF in some samples, although at a lesser extent than DGC, suggesting that it should be used to select spermatozoa for ARTs when possible." @default.
• W2946436563 created "2019-05-29" @default.
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• W2946436563 date "2019-05-16" @default.
• W2946436563 modified "2023-10-14" @default.
• W2946436563 title "Sperm selection with density gradient centrifugation and swim up: effect on DNA fragmentation in viable spermatozoa" @default.
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• W2946436563 doi "https://doi.org/10.1038/s41598-019-43981-2" @default.
• W2946436563 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6522556" @default.
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