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- W2946473592 abstract "Interrogating non-coding RNA structures and functions with small molecules is an area of rapidly increasing interest among biochemists and chemical biologists. However, many biochemical approaches to monitoring RNA structures are time-consuming and low-throughput, and thereby are only of limited utility for RNA-small molecule studies. Fluorescence-based techniques are powerful tools for rapid investigation of RNA conformations, dynamics, and interactions with small molecules. Many fluorescence methods are amenable to high-throughput analysis, enabling library screening for small molecule binders. In this review, we summarize numerous fluorescence-based approaches for identifying and characterizing RNA-small molecule interactions. We describe in detail a high-information content dual-reporter FRET assay we developed to characterize small molecule-induced conformational and stability changes. Our assay is uniquely suited as a platform for both small molecule discovery and thorough characterization of RNA-small molecule binding mechanisms. Given the growing recognition of non-coding RNAs as attractive targets for therapeutic intervention, we anticipate our FRET assay and other fluorescence-based techniques will be indispensable for the development of potent and specific small molecule inhibitors targeting RNA." @default.
- W2946473592 created "2019-05-29" @default.
- W2946473592 creator A5035692707 @default.
- W2946473592 creator A5038637180 @default.
- W2946473592 date "2019-09-01" @default.
- W2946473592 modified "2023-09-26" @default.
- W2946473592 title "Fluorescence-based investigations of RNA-small molecule interactions" @default.
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- W2946473592 doi "https://doi.org/10.1016/j.ymeth.2019.05.017" @default.
- W2946473592 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6756994" @default.
- W2946473592 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/31129289" @default.
- W2946473592 hasPublicationYear "2019" @default.
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