FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2946585144> ?p ?o ?g. }

• W2946585144 abstract "In cows with high milk production, negative relationships between negative energy balance (NEB), inducing non-esterified fatty acids (NEFAs) in the peripheral circulation, and reproductive performances are well documented. Three major NEFAs; oleic (OA), palmitic (PA) and stearic acids (SA) have adverse effects on reproductive cells. In this thesis, the effects of NEB on gene expression in specific endometrial cells were studied from an in vivo model. Full endometrial biopsies were collected from Swedish Red Breed cows with different NEB status at 80 days postpartum. The three types of endometrial cells (stroma; ST, glandular epithelium; GE, and luminal epithelium; LE) were isolated from a tissue sample by using laser microdissection. Cell type-specific gene expression was identified in those cell types by RNA sequencing. The effects of the severity of NEB on endometrial gene expression were identified and it was found that the response to NEB was specific in the different cell types. Changes in gene expression are more profound in ST in which genes encoding proteins involved in energy metabolism, cell structure and peri-implantation processes were affected. Over-expression of genes associated with inflammatory response was observed in GE, whereas under-expression of genes related to adaptive immunity was observed in LE. In an in vitro study, we investigated the effects of the three NEFAs on phenotypic and gene expression responses of bovine endometrial epithelial cells (bEECs) in culture. Cultured postprimary bEECs were exposed to different concentrations of OA, PA and SA or their mixture. Cell viability, proliferation, apoptosis, lipid accumulation, pro-inflammatory cytokines (IL-6 and IL-8) production were examined at 24 and 48 h. Elevated NEFA dosage decreased cell viability and proliferation, while apoptosis, lipid accumulation and cytokine production were increased. The negative effects of OA and PA were stronger than those induced by SA. Gene expression changes of bEECs induced by 300 µM of either OA or PA for 2 h were further determined by using RNA sequencing. Oleic acid induced stronger changes in gene expression than PA. The up-regulation of pathways related to energy metabolism, apoptosis and oxidative stress signaling were observed whereas significance was not reached following use of PA. In conclusion, these results indicate that metabolic stress can affect endometrial functions in various ways. The gene expression changes in vivo suggests that severe NEB have long term effects on endometrial cells and that the responses are specific to cell types. The results from in vitro studies clearly show that NEFAs, especially OA and PA, alters many functions of bEECs and are detrimental to the sub-population of epithelial cells. Taken together, the results document that metabolic imbalance during the post-partum period alters several functions in the endometrium in a specific way in the different cell types and strongly stimulate pro-inflammatory mechanisms especially in epithelial cells." @default.
• W2946585144 created "2019-05-29" @default.
• W2946585144 creator A5044361902 @default.
• W2946585144 date "2019-01-01" @default.
• W2946585144 modified "2023-09-26" @default.
• W2946585144 title "Impacts of fat mobilization on bovine endometrium function" @default.
• W2946585144 cites W155326310 @default.
• W2946585144 cites W1667057875 @default.
• W2946585144 cites W190113581 @default.
• W2946585144 cites W1919166337 @default.
• W2946585144 cites W1964775850 @default.
• W2946585144 cites W1965114400 @default.
• W2946585144 cites W1966727900 @default.
• W2946585144 cites W1967007996 @default.
• W2946585144 cites W1967825073 @default.
• W2946585144 cites W1968692066 @default.
• W2946585144 cites W1970714799 @default.
• W2946585144 cites W1973796897 @default.
• W2946585144 cites W1975569188 @default.
• W2946585144 cites W1975933478 @default.
• W2946585144 cites W1977404830 @default.
• W2946585144 cites W1982510502 @default.
• W2946585144 cites W1983776515 @default.
• W2946585144 cites W1984750471 @default.
• W2946585144 cites W1986804947 @default.
• W2946585144 cites W1987840126 @default.
• W2946585144 cites W1993243925 @default.
• W2946585144 cites W1995048704 @default.
• W2946585144 cites W1996032049 @default.
• W2946585144 cites W1996814033 @default.
• W2946585144 cites W2009139770 @default.
• W2946585144 cites W2009421311 @default.
• W2946585144 cites W2010412784 @default.
• W2946585144 cites W2011954953 @default.
• W2946585144 cites W2012099404 @default.
• W2946585144 cites W2015877850 @default.
• W2946585144 cites W2017093116 @default.
• W2946585144 cites W2019512263 @default.
• W2946585144 cites W2021604124 @default.
• W2946585144 cites W2021807548 @default.
• W2946585144 cites W2022069296 @default.
• W2946585144 cites W2024355922 @default.
• W2946585144 cites W2024508748 @default.
• W2946585144 cites W2025416246 @default.
• W2946585144 cites W2025483003 @default.
• W2946585144 cites W2025925423 @default.
• W2946585144 cites W2027737545 @default.
• W2946585144 cites W2031922822 @default.
• W2946585144 cites W2033037262 @default.
• W2946585144 cites W2034166355 @default.
• W2946585144 cites W2034216887 @default.
• W2946585144 cites W2038191577 @default.
• W2946585144 cites W2038961236 @default.
• W2946585144 cites W2040772593 @default.
• W2946585144 cites W2042354837 @default.
• W2946585144 cites W2043011037 @default.
• W2946585144 cites W2050875594 @default.
• W2946585144 cites W2051611159 @default.
• W2946585144 cites W2056422600 @default.
• W2946585144 cites W2056675167 @default.
• W2946585144 cites W2063989843 @default.
• W2946585144 cites W2069403965 @default.
• W2946585144 cites W2073168934 @default.
• W2946585144 cites W2074404034 @default.
• W2946585144 cites W2075185675 @default.
• W2946585144 cites W2083943383 @default.
• W2946585144 cites W2085226735 @default.
• W2946585144 cites W2092328207 @default.
• W2946585144 cites W2100745995 @default.
• W2946585144 cites W2102428962 @default.
• W2946585144 cites W2109004108 @default.
• W2946585144 cites W2113646062 @default.
• W2946585144 cites W2117299685 @default.
• W2946585144 cites W2118080808 @default.
• W2946585144 cites W2120480222 @default.
• W2946585144 cites W2120989351 @default.
• W2946585144 cites W2121212588 @default.
• W2946585144 cites W2122909568 @default.
• W2946585144 cites W2124343538 @default.
• W2946585144 cites W2127542959 @default.
• W2946585144 cites W2131250377 @default.
• W2946585144 cites W2132326233 @default.
• W2946585144 cites W2133449992 @default.
• W2946585144 cites W2134720556 @default.
• W2946585144 cites W2137100991 @default.
• W2946585144 cites W2137656616 @default.
• W2946585144 cites W2142714475 @default.
• W2946585144 cites W2143108062 @default.
• W2946585144 cites W2145050141 @default.
• W2946585144 cites W2146689228 @default.
• W2946585144 cites W2147995078 @default.
• W2946585144 cites W2150801318 @default.
• W2946585144 cites W2153627831 @default.
• W2946585144 cites W2154414030 @default.
• W2946585144 cites W2155400649 @default.
• W2946585144 cites W2155642459 @default.
• W2946585144 cites W2156608568 @default.
• W2946585144 cites W2158079900 @default.
• W2946585144 cites W2159660882 @default.
• W2946585144 cites W2160535648 @default.

• next