FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2946589813> ?p ?o ?g. }

• W2946589813 endingPage "e3" @default.
• W2946589813 startingPage "e3" @default.
• W2946589813 abstract "Background & Aim Accumulating evidence indicates that extracellular vesicles (EVs) derived from mesenchymal stem/stromal cells (MSCs) have immunomodulatory effects. Although the molecular mechanisms underlying the EV-mediated immunomodulation have not been fully understood, several studies demonstrated that MSC-derived EVs (MSC-EVs) carry numerous numbers of mRNAs, microRNAs, and proteins and these factors are responsible for the therapeutic effect of MSC-EVs. However, the contents of MSC-EVs can change as their parent cell changes since EVs carry a subset of proteins and RNAs of their parent cell, suggesting that the therapeutic efficacy of MSC-EVs can be also varied depending on donors and culture conditions of MSCs. Therefore, the aim of our study is to establish a molecular profile of functionally effective MSC-EVs in immunomodulation. Methods, Results & Conclusion MSCs lose many of their biological properties as they are expanded in culture, we therefore compared immunomodulatory effects of EVs derived from early passage (passage 2: P2) MSCs and late passage (P7) MSCs using splenocyte cultures and mice with ocular Sjögren's syndrome. In parallel, we performed proteomics and RNA sequencing with both of the EVs. We found that the P2 EVs more effectively suppressed the activation of splenocytes in vitro and improved inflammatory dry eye in mice with ocular Sjögren's syndrome. Proteomics showed that about 40% of proteins enriched in both P2 and P7 EVs were binding proteins, but the top ranked binding proteins of the P2 EVs were classified as extracellular region or matrix proteins while P7 EV binding proteins were classified as chromatin or nucleic acid binding proteins. In addition, the well-known immunomodulators, TGFβ1 and PTX3 (Pentraxin 3) were enriched in the P2 EVs and inhibition or overexpression of TGFβ1 or PTX3 in MSCs directly affected the immunomodulatory potency of their EVs. Moreover, RNA sequencing revealed that about 80 miRNAs were differentially expressed between P2 and P7 EVs and the well-known immunomodulators, let-7b-5p and miR-146b were also enriched in P2 EVs. In summary, our comparative analyses between EVs from early and late passage MSCs provided the distinctive molecular profile of functionally effective MSC-EVs and also helped understand the molecular mechanism underlying the EV-mediated immunomodulation. Furthermore, our data suggest that the enriched factors in functionally effective EVs can be used as biomarkers to evaluate their regulatory function in immunomodulation. Accumulating evidence indicates that extracellular vesicles (EVs) derived from mesenchymal stem/stromal cells (MSCs) have immunomodulatory effects. Although the molecular mechanisms underlying the EV-mediated immunomodulation have not been fully understood, several studies demonstrated that MSC-derived EVs (MSC-EVs) carry numerous numbers of mRNAs, microRNAs, and proteins and these factors are responsible for the therapeutic effect of MSC-EVs. However, the contents of MSC-EVs can change as their parent cell changes since EVs carry a subset of proteins and RNAs of their parent cell, suggesting that the therapeutic efficacy of MSC-EVs can be also varied depending on donors and culture conditions of MSCs. Therefore, the aim of our study is to establish a molecular profile of functionally effective MSC-EVs in immunomodulation. MSCs lose many of their biological properties as they are expanded in culture, we therefore compared immunomodulatory effects of EVs derived from early passage (passage 2: P2) MSCs and late passage (P7) MSCs using splenocyte cultures and mice with ocular Sjögren's syndrome. In parallel, we performed proteomics and RNA sequencing with both of the EVs." @default.
• W2946589813 created "2019-05-29" @default.
• W2946589813 creator A5020034175 @default.
• W2946589813 creator A5024071586 @default.
• W2946589813 creator A5024570164 @default.
• W2946589813 creator A5042345374 @default.
• W2946589813 creator A5047446402 @default.
• W2946589813 creator A5064046139 @default.
• W2946589813 creator A5074974283 @default.
• W2946589813 creator A5081254728 @default.
• W2946589813 creator A5086030245 @default.
• W2946589813 date "2019-05-01" @default.
• W2946589813 modified "2023-10-17" @default.
• W2946589813 title "Molecular profiles of functionally effective MSC-Derived extracellular vesicles in immunomodulation" @default.
• W2946589813 doi "https://doi.org/10.1016/j.jcyt.2019.04.011" @default.
• W2946589813 hasPublicationYear "2019" @default.
• W2946589813 type Work @default.
• W2946589813 sameAs 2946589813 @default.
• W2946589813 citedByCount "1" @default.
• W2946589813 countsByYear W29465898132023 @default.
• W2946589813 crossrefType "journal-article" @default.
• W2946589813 hasAuthorship W2946589813A5020034175 @default.
• W2946589813 hasAuthorship W2946589813A5024071586 @default.
• W2946589813 hasAuthorship W2946589813A5024570164 @default.
• W2946589813 hasAuthorship W2946589813A5042345374 @default.
• W2946589813 hasAuthorship W2946589813A5047446402 @default.
• W2946589813 hasAuthorship W2946589813A5064046139 @default.
• W2946589813 hasAuthorship W2946589813A5074974283 @default.
• W2946589813 hasAuthorship W2946589813A5081254728 @default.
• W2946589813 hasAuthorship W2946589813A5086030245 @default.
• W2946589813 hasConcept C104317684 @default.
• W2946589813 hasConcept C145059251 @default.
• W2946589813 hasConcept C16930146 @default.
• W2946589813 hasConcept C185592680 @default.
• W2946589813 hasConcept C189165786 @default.
• W2946589813 hasConcept C189976730 @default.
• W2946589813 hasConcept C198826908 @default.
• W2946589813 hasConcept C202751555 @default.
• W2946589813 hasConcept C20518536 @default.
• W2946589813 hasConcept C2781261824 @default.
• W2946589813 hasConcept C2908689518 @default.
• W2946589813 hasConcept C2992929900 @default.
• W2946589813 hasConcept C46111723 @default.
• W2946589813 hasConcept C502942594 @default.
• W2946589813 hasConcept C55493867 @default.
• W2946589813 hasConcept C86803240 @default.
• W2946589813 hasConcept C95444343 @default.
• W2946589813 hasConceptScore W2946589813C104317684 @default.
• W2946589813 hasConceptScore W2946589813C145059251 @default.
• W2946589813 hasConceptScore W2946589813C16930146 @default.
• W2946589813 hasConceptScore W2946589813C185592680 @default.
• W2946589813 hasConceptScore W2946589813C189165786 @default.
• W2946589813 hasConceptScore W2946589813C189976730 @default.
• W2946589813 hasConceptScore W2946589813C198826908 @default.
• W2946589813 hasConceptScore W2946589813C202751555 @default.
• W2946589813 hasConceptScore W2946589813C20518536 @default.
• W2946589813 hasConceptScore W2946589813C2781261824 @default.
• W2946589813 hasConceptScore W2946589813C2908689518 @default.
• W2946589813 hasConceptScore W2946589813C2992929900 @default.
• W2946589813 hasConceptScore W2946589813C46111723 @default.
• W2946589813 hasConceptScore W2946589813C502942594 @default.
• W2946589813 hasConceptScore W2946589813C55493867 @default.
• W2946589813 hasConceptScore W2946589813C86803240 @default.
• W2946589813 hasConceptScore W2946589813C95444343 @default.
• W2946589813 hasIssue "5" @default.
• W2946589813 hasLocation W29465898131 @default.
• W2946589813 hasOpenAccess W2946589813 @default.
• W2946589813 hasPrimaryLocation W29465898131 @default.
• W2946589813 hasRelatedWork W2060135922 @default.
• W2946589813 hasRelatedWork W2732249677 @default.
• W2946589813 hasRelatedWork W2889023565 @default.
• W2946589813 hasRelatedWork W2917685500 @default.
• W2946589813 hasRelatedWork W4224308415 @default.
• W2946589813 hasRelatedWork W4224750750 @default.
• W2946589813 hasRelatedWork W4307400404 @default.
• W2946589813 hasRelatedWork W4376112206 @default.
• W2946589813 hasRelatedWork W4376112267 @default.
• W2946589813 hasRelatedWork W4376112317 @default.
• W2946589813 hasVolume "21" @default.
• W2946589813 isParatext "false" @default.
• W2946589813 isRetracted "false" @default.
• W2946589813 magId "2946589813" @default.
• W2946589813 workType "article" @default.