Matches in SemOpenAlex for { <https://semopenalex.org/work/W2946633074> ?p ?o ?g. }
- W2946633074 endingPage "1429" @default.
- W2946633074 startingPage "1421" @default.
- W2946633074 abstract "Engineering polymerases to synthesize artificial genetic polymers with unique backbone structures is limited by a general lack of understanding about the structural determinants that govern substrate specificity. Here, we report a high-throughput microfluidic-based approach for mapping sequence–function relationships that combines droplet-based optical polymerase sorting with deep mutational scanning. We applied this strategy to map the finger subdomain of a replicative DNA polymerase isolated from Thermococcus kodakarensis (Kod). The enrichment profile provides an unbiased view of the ability of each mutant to synthesize threose nucleic acid, which was used as a model non-natural genetic polymer. From a single round of sorting, we discovered two cases of positive epistasis and demonstrate the near inversion of substrate specificity from a double mutant variant. This effort indicates that polymerase specificity may be governed by a small number of highly specific residues that can be elucidated by deep mutational scanning without the need for iterative rounds of directed evolution." @default.
- W2946633074 created "2019-05-29" @default.
- W2946633074 creator A5018259778 @default.
- W2946633074 creator A5071896807 @default.
- W2946633074 creator A5081890628 @default.
- W2946633074 date "2019-05-13" @default.
- W2946633074 modified "2023-10-06" @default.
- W2946633074 title "Elucidating the Determinants of Polymerase Specificity by Microfluidic-Based Deep Mutational Scanning" @default.
- W2946633074 cites W1493541064 @default.
- W2946633074 cites W1517927116 @default.
- W2946633074 cites W1533050665 @default.
- W2946633074 cites W1971364137 @default.
- W2946633074 cites W1989527063 @default.
- W2946633074 cites W1998588829 @default.
- W2946633074 cites W2002632700 @default.
- W2946633074 cites W2003378333 @default.
- W2946633074 cites W2011562612 @default.
- W2946633074 cites W2025962542 @default.
- W2946633074 cites W2044801589 @default.
- W2946633074 cites W2045074917 @default.
- W2946633074 cites W2045974535 @default.
- W2946633074 cites W2050530360 @default.
- W2946633074 cites W2056395175 @default.
- W2946633074 cites W2060588922 @default.
- W2946633074 cites W2066423863 @default.
- W2946633074 cites W2068659296 @default.
- W2946633074 cites W2072010276 @default.
- W2946633074 cites W2076946539 @default.
- W2946633074 cites W2089142368 @default.
- W2946633074 cites W2089891949 @default.
- W2946633074 cites W2135636849 @default.
- W2946633074 cites W2152763806 @default.
- W2946633074 cites W2158207944 @default.
- W2946633074 cites W2227529953 @default.
- W2946633074 cites W2267839754 @default.
- W2946633074 cites W2291221808 @default.
- W2946633074 cites W2335690990 @default.
- W2946633074 cites W2336955395 @default.
- W2946633074 cites W2471654536 @default.
- W2946633074 cites W2604656037 @default.
- W2946633074 cites W2750714005 @default.
- W2946633074 cites W2752138982 @default.
- W2946633074 cites W2769440634 @default.
- W2946633074 cites W2770511541 @default.
- W2946633074 cites W2774557158 @default.
- W2946633074 cites W2800130163 @default.
- W2946633074 cites W2900904251 @default.
- W2946633074 doi "https://doi.org/10.1021/acssynbio.9b00104" @default.
- W2946633074 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/31081325" @default.
- W2946633074 hasPublicationYear "2019" @default.
- W2946633074 type Work @default.
- W2946633074 sameAs 2946633074 @default.
- W2946633074 citedByCount "30" @default.
- W2946633074 countsByYear W29466330742019 @default.
- W2946633074 countsByYear W29466330742020 @default.
- W2946633074 countsByYear W29466330742021 @default.
- W2946633074 countsByYear W29466330742022 @default.
- W2946633074 countsByYear W29466330742023 @default.
- W2946633074 crossrefType "journal-article" @default.
- W2946633074 hasAuthorship W2946633074A5018259778 @default.
- W2946633074 hasAuthorship W2946633074A5071896807 @default.
- W2946633074 hasAuthorship W2946633074A5081890628 @default.
- W2946633074 hasConcept C104317684 @default.
- W2946633074 hasConcept C143065580 @default.
- W2946633074 hasConcept C54355233 @default.
- W2946633074 hasConcept C552990157 @default.
- W2946633074 hasConcept C70721500 @default.
- W2946633074 hasConcept C82381507 @default.
- W2946633074 hasConcept C86803240 @default.
- W2946633074 hasConcept C9418097 @default.
- W2946633074 hasConceptScore W2946633074C104317684 @default.
- W2946633074 hasConceptScore W2946633074C143065580 @default.
- W2946633074 hasConceptScore W2946633074C54355233 @default.
- W2946633074 hasConceptScore W2946633074C552990157 @default.
- W2946633074 hasConceptScore W2946633074C70721500 @default.
- W2946633074 hasConceptScore W2946633074C82381507 @default.
- W2946633074 hasConceptScore W2946633074C86803240 @default.
- W2946633074 hasConceptScore W2946633074C9418097 @default.
- W2946633074 hasFunder F4320332180 @default.
- W2946633074 hasFunder F4320337397 @default.
- W2946633074 hasIssue "6" @default.
- W2946633074 hasLocation W29466330741 @default.
- W2946633074 hasLocation W29466330742 @default.
- W2946633074 hasOpenAccess W2946633074 @default.
- W2946633074 hasPrimaryLocation W29466330741 @default.
- W2946633074 hasRelatedWork W1969421403 @default.
- W2946633074 hasRelatedWork W2002128513 @default.
- W2946633074 hasRelatedWork W2064207257 @default.
- W2946633074 hasRelatedWork W2128665526 @default.
- W2946633074 hasRelatedWork W2150065613 @default.
- W2946633074 hasRelatedWork W2156423843 @default.
- W2946633074 hasRelatedWork W2156674132 @default.
- W2946633074 hasRelatedWork W4246662077 @default.
- W2946633074 hasRelatedWork W4247436401 @default.
- W2946633074 hasRelatedWork W2092874662 @default.
- W2946633074 hasVolume "8" @default.
- W2946633074 isParatext "false" @default.
- W2946633074 isRetracted "false" @default.