Matches in SemOpenAlex for { <https://semopenalex.org/work/W2946642010> ?p ?o ?g. }
Showing items 1 to 91 of
91
with 100 items per page.
- W2946642010 endingPage "261" @default.
- W2946642010 startingPage "256" @default.
- W2946642010 abstract "Currently, a therapeutic drug based on recombinant antibodies for the prevention and treatment of tick-borne encephalitis virus (TBEV) is developed in ICBFM SB RAS, and the chimeric antibody ch14D5 is considered as one of the key components of this drug. It was previously shown that this antibody is directed to the domain D3 of the glycoprotein E of TBEV. It was previously shown that this antibody is able to protect mice from the European subtype of TBEV, strain “Absettarov”, and the presence of virus-neutralizing activity against the Far Eastern subtype of TBEV, strain 205 was also shown for this antibody. However, it remains unclear whether this antibody exhibits selectivity for different subtypes of TBEV. The aim of this study was to investigate the effect of amino acid sequence differences of recombinant D3 domains derived from the glycoprotein E of TBEV of the Far Eastern, Siberian and European subtypes on the binding of the protective antibody ch14D5 to these proteins. Using Western blot analysis and surface plasmon resonance, it was shown that ch14D5 antibody has the highest affinity (K D = 1.7±0.5 nM) for the D3 domain of the TBEV of the “Sofjin-Ru” strain belonging to the Far Eastern subtype of the virus. At the same time, the affinity of ch14D5 antibody for similar D3 proteins derived from “Zausaev”, “1528-99” and “Absettarov” strains of the Siberian and European subtypes of TBEV was noticeably lower (K D = 25±4, 300±50, 250±50 nM, respectively). In addition, information about the spatial arrangement of amino acid residues that are different for the studied recombinant proteins indicates that the epitope recognized by the ch14D5 antibody is in close proximity to the lateral ridge of D3 domain of E glycoprotein." @default.
- W2946642010 created "2019-05-29" @default.
- W2946642010 creator A5023005705 @default.
- W2946642010 creator A5032999168 @default.
- W2946642010 creator A5044148511 @default.
- W2946642010 creator A5066382342 @default.
- W2946642010 creator A5073143628 @default.
- W2946642010 creator A5080652825 @default.
- W2946642010 date "2019-05-14" @default.
- W2946642010 modified "2023-10-06" @default.
- W2946642010 title "The effect of differences in the third domain of the glycoprotein E of tick-borne encephalitis virus of the Far Eastern, Siberian and European subtypes on the binding of recombinant D3 proteins with a chimeric antibody" @default.
- W2946642010 cites W1984015851 @default.
- W2946642010 cites W1998644452 @default.
- W2946642010 cites W2005864470 @default.
- W2946642010 cites W2018347496 @default.
- W2946642010 cites W2037544387 @default.
- W2946642010 cites W2065486680 @default.
- W2946642010 cites W2074439419 @default.
- W2946642010 cites W2086577162 @default.
- W2946642010 cites W2126624392 @default.
- W2946642010 cites W2151551773 @default.
- W2946642010 cites W2346452525 @default.
- W2946642010 cites W2516118470 @default.
- W2946642010 cites W2727086257 @default.
- W2946642010 cites W2755477691 @default.
- W2946642010 cites W2785543194 @default.
- W2946642010 cites W2811437092 @default.
- W2946642010 cites W4233906842 @default.
- W2946642010 doi "https://doi.org/10.18699/vj19.490" @default.
- W2946642010 hasPublicationYear "2019" @default.
- W2946642010 type Work @default.
- W2946642010 sameAs 2946642010 @default.
- W2946642010 citedByCount "3" @default.
- W2946642010 countsByYear W29466420102021 @default.
- W2946642010 countsByYear W29466420102022 @default.
- W2946642010 crossrefType "journal-article" @default.
- W2946642010 hasAuthorship W2946642010A5023005705 @default.
- W2946642010 hasAuthorship W2946642010A5032999168 @default.
- W2946642010 hasAuthorship W2946642010A5044148511 @default.
- W2946642010 hasAuthorship W2946642010A5066382342 @default.
- W2946642010 hasAuthorship W2946642010A5073143628 @default.
- W2946642010 hasAuthorship W2946642010A5080652825 @default.
- W2946642010 hasBestOaLocation W29466420101 @default.
- W2946642010 hasConcept C104317684 @default.
- W2946642010 hasConcept C108625454 @default.
- W2946642010 hasConcept C153911025 @default.
- W2946642010 hasConcept C159047783 @default.
- W2946642010 hasConcept C159654299 @default.
- W2946642010 hasConcept C203014093 @default.
- W2946642010 hasConcept C2522874641 @default.
- W2946642010 hasConcept C2776281056 @default.
- W2946642010 hasConcept C2776415932 @default.
- W2946642010 hasConcept C40767141 @default.
- W2946642010 hasConcept C55493867 @default.
- W2946642010 hasConcept C83455156 @default.
- W2946642010 hasConcept C86803240 @default.
- W2946642010 hasConceptScore W2946642010C104317684 @default.
- W2946642010 hasConceptScore W2946642010C108625454 @default.
- W2946642010 hasConceptScore W2946642010C153911025 @default.
- W2946642010 hasConceptScore W2946642010C159047783 @default.
- W2946642010 hasConceptScore W2946642010C159654299 @default.
- W2946642010 hasConceptScore W2946642010C203014093 @default.
- W2946642010 hasConceptScore W2946642010C2522874641 @default.
- W2946642010 hasConceptScore W2946642010C2776281056 @default.
- W2946642010 hasConceptScore W2946642010C2776415932 @default.
- W2946642010 hasConceptScore W2946642010C40767141 @default.
- W2946642010 hasConceptScore W2946642010C55493867 @default.
- W2946642010 hasConceptScore W2946642010C83455156 @default.
- W2946642010 hasConceptScore W2946642010C86803240 @default.
- W2946642010 hasIssue "3" @default.
- W2946642010 hasLocation W29466420101 @default.
- W2946642010 hasLocation W29466420102 @default.
- W2946642010 hasOpenAccess W2946642010 @default.
- W2946642010 hasPrimaryLocation W29466420101 @default.
- W2946642010 hasRelatedWork W1978023727 @default.
- W2946642010 hasRelatedWork W1978922829 @default.
- W2946642010 hasRelatedWork W1996144625 @default.
- W2946642010 hasRelatedWork W1999501264 @default.
- W2946642010 hasRelatedWork W2009002440 @default.
- W2946642010 hasRelatedWork W2018681560 @default.
- W2946642010 hasRelatedWork W2023089872 @default.
- W2946642010 hasRelatedWork W2128694535 @default.
- W2946642010 hasRelatedWork W2360587303 @default.
- W2946642010 hasRelatedWork W2465804977 @default.
- W2946642010 hasVolume "23" @default.
- W2946642010 isParatext "false" @default.
- W2946642010 isRetracted "false" @default.
- W2946642010 magId "2946642010" @default.
- W2946642010 workType "article" @default.