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- W2946730229 abstract "A rapid and sensitive TaqMan real-time PCR assay were developed for the detection of gadiformes.For high gadiformes specificity,the primers and the probe were designed on the basis of the mitochondrial 16S rRNA gene.The detection limit of this real-time PCR assay was 0.01 ng/μl DNA concentration and 0.01%(W/W)gadiformes meat powder.To confirm the specificity of these methods,genomic DNA samples from 71other species were examined;no amplification products were detected.Thus,the method shows high sensitivity and may be applicable as specific tools for the detection of trace amounts of gadiformes in commercial food products." @default.
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- W2946730229 date "2012-12-25" @default.
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- W2946730229 title "Detection of Gadiformes by Real-time PCR Assay" @default.
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