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- W2946876102 startingPage "81" @default.
- W2946876102 abstract "Astroglia are vital facilitators of brain development, homeostasis, and metabolic support. In addition, they are also essential to the formation and regulation of synaptic circuits. Due to the extraordinary complex, nanoscopic morphology of astrocytes, the underlying cellular mechanisms have been poorly understood. In particular, fine astrocytic processes that can be found in the vicinity of synapses have been difficult to study using traditional imaging techniques. Here, we describe a 3D three-colour super-resolution microscopy approach to unravel the nanostructure of tripartite synapses. The method is based on the SMLM technique direct stochastic optical reconstruction microscopy (dSTORM) which uses conventional fluorophore-labelled antibodies. This approach enables reconstructing the nanoscale localisation of individual astrocytic glutamate transporter (GLT-1) molecules surrounding presynaptic (bassoon) and postsynaptic (Homer1) protein localisations in fixed mouse brain sections. However, the technique is readily adaptable to other types of targets and tissues." @default.
- W2946876102 created "2019-06-07" @default.
- W2946876102 creator A5015137977 @default.
- W2946876102 creator A5024444613 @default.
- W2946876102 creator A5053437342 @default.
- W2946876102 creator A5090829486 @default.
- W2946876102 date "2020-03-01" @default.
- W2946876102 modified "2023-10-17" @default.
- W2946876102 title "Imaging tripartite synapses using super-resolution microscopy" @default.
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