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- W2946890936 abstract "Elimination of malaria depends on mastering transmission and understanding the biological basis of Plasmodium infection in the vector. The first mosquito organ to interact with the parasite is the midgut and its transcriptomic characterization during infection can reveal effective antiplasmodial responses able to limit the survival of the parasite. The vector response to Plasmodium vivax is not fully characterized, and its specificities when compared with other malaria parasites can be of fundamental interest for specific control measures. Experimental infections were performed using a membrane-feeding device. Three groups were used: P. vivax-blood-fed, blood-fed on inactivated gametocytes, and unfed mosquitoes. Twenty-four hours after feeding, the mosquitoes were dissected and the midgut collected for transcriptomic analysis using RNAseq. Nine cDNA libraries were generated and sequenced on an Illumina HiSeq2500. Readings were checked for quality control and analysed using the Trinity platform for de novo transcriptome assembly. Transcript quantification was performed and the transcriptome was functionally annotated. Differential expression gene analysis was carried out. The role of the identified mechanisms was further explored using functional approaches. Forty-nine genes were identified as being differentially expressed with P. vivax infection: 34 were upregulated and 15 were downregulated. Half of the P. vivax-related differentially expressed genes could be related to autophagy; therefore, the effect of the known inhibitor (wortmannin) and activator (spermidine) was tested on the infection outcome. Autophagic activation significantly reduced the intensity and prevalence of infection. This was associated with transcription alterations of the autophagy regulating genes Beclin, DRAM and Apg8. Our data indicate that P. vivax invasion of An. aquasalis midgut epithelium triggers an autophagic response and its activation reduces infection. This suggests a novel mechanism that mosquitoes can use to fight Plasmodium infection." @default.
- W2946890936 created "2019-06-07" @default.
- W2946890936 creator A5001446475 @default.
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- W2946890936 date "2019-05-24" @default.
- W2946890936 modified "2023-10-11" @default.
- W2946890936 title "Anopheles aquasalis transcriptome reveals autophagic responses to Plasmodium vivax midgut invasion" @default.
- W2946890936 cites W1498806970 @default.
- W2946890936 cites W1548474475 @default.
- W2946890936 cites W1854421050 @default.
- W2946890936 cites W1897349928 @default.
- W2946890936 cites W1966740762 @default.
- W2946890936 cites W1972693900 @default.
- W2946890936 cites W1973130575 @default.
- W2946890936 cites W1974152703 @default.
- W2946890936 cites W1977524410 @default.
- W2946890936 cites W1978451782 @default.
- W2946890936 cites W1982362155 @default.
- W2946890936 cites W1984387702 @default.
- W2946890936 cites W1984857335 @default.
- W2946890936 cites W1985334939 @default.
- W2946890936 cites W1986932941 @default.
- W2946890936 cites W1991932216 @default.
- W2946890936 cites W1999574084 @default.
- W2946890936 cites W2006740007 @default.
- W2946890936 cites W2011657487 @default.
- W2946890936 cites W2023356295 @default.
- W2946890936 cites W2024784927 @default.
- W2946890936 cites W2025665129 @default.
- W2946890936 cites W2025718072 @default.
- W2946890936 cites W2025845443 @default.
- W2946890936 cites W2027928475 @default.
- W2946890936 cites W2028965920 @default.
- W2946890936 cites W2032997281 @default.
- W2946890936 cites W2036203075 @default.
- W2946890936 cites W2039403501 @default.
- W2946890936 cites W2042958174 @default.
- W2946890936 cites W2044166038 @default.
- W2946890936 cites W2046006262 @default.
- W2946890936 cites W2048663311 @default.
- W2946890936 cites W2050449054 @default.
- W2946890936 cites W2053279636 @default.
- W2946890936 cites W2057400761 @default.
- W2946890936 cites W2059107485 @default.
- W2946890936 cites W2060441844 @default.
- W2946890936 cites W2062048451 @default.
- W2946890936 cites W2065031087 @default.
- W2946890936 cites W2075707505 @default.
- W2946890936 cites W2075927731 @default.
- W2946890936 cites W2076119324 @default.
- W2946890936 cites W2077373845 @default.
- W2946890936 cites W2082781025 @default.
- W2946890936 cites W2089061917 @default.
- W2946890936 cites W2089563834 @default.
- W2946890936 cites W2089728480 @default.
- W2946890936 cites W2090872177 @default.
- W2946890936 cites W2093765409 @default.
- W2946890936 cites W2094503313 @default.
- W2946890936 cites W2095696895 @default.
- W2946890936 cites W2098264151 @default.
- W2946890936 cites W2098921256 @default.
- W2946890936 cites W2108205859 @default.
- W2946890936 cites W2112149015 @default.
- W2946890936 cites W2114104545 @default.
- W2946890936 cites W2124985265 @default.
- W2946890936 cites W2129712803 @default.
- W2946890936 cites W2142633440 @default.
- W2946890936 cites W2145983008 @default.
- W2946890936 cites W2150027419 @default.
- W2946890936 cites W2156395269 @default.
- W2946890936 cites W2157778451 @default.
- W2946890936 cites W2163018203 @default.
- W2946890936 cites W2164998112 @default.
- W2946890936 cites W2175807643 @default.
- W2946890936 cites W2184715874 @default.
- W2946890936 cites W2395979148 @default.
- W2946890936 cites W2463442958 @default.
- W2946890936 cites W2465759754 @default.
- W2946890936 cites W2528687860 @default.
- W2946890936 cites W2530590997 @default.
- W2946890936 cites W2559466477 @default.
- W2946890936 cites W2560004176 @default.
- W2946890936 cites W2602035017 @default.
- W2946890936 cites W2606578016 @default.
- W2946890936 cites W2624269063 @default.
- W2946890936 cites W2781900985 @default.
- W2946890936 cites W2789655196 @default.
- W2946890936 cites W2808422876 @default.
- W2946890936 cites W2897069969 @default.
- W2946890936 cites W2902334063 @default.
- W2946890936 doi "https://doi.org/10.1186/s13071-019-3506-8" @default.