Matches in SemOpenAlex for { <https://semopenalex.org/work/W2946916693> ?p ?o ?g. }
Showing items 1 to 90 of
90
with 100 items per page.
- W2946916693 abstract "Introduction: Polymethylmethacrylate resins are essential materials in the Oral Rehabilitation field. However, their use is being called into question once they can cause adverse reactions in the adjacent tissues [1]. Previous work has shown that ProBase® Cold releases a higher amount of monomers MMA and HEMA when compared to the heat-polymerized ProBase® Hot [2]. Having that in consideration, it is important to know if polymerization methods have influence in cytotoxicity of these materials. In this in vitro study, our aim is to evaluate the cytotoxicity level of ProBase® Hot and ProBase® Cold in fibroblastic cell line through cell viability assay and our hypothesis is that ProBase® Cold is more cytotoxic than ProBase® Hot.Materials and methods: Specimens of each resin were prepared according to manufacturer’s instructions for clinical use. Then, 4 disks (5mm in diameter x 2mm in height) were immersed in 7mL of culture medium and incubated for 4 different times (30 minutes, 24 hours, 7 days and 14 days). Serial dilutions of the previously incubated resin extracts were placed in contact with 4×103 of 3T3 mouse fibroblasts that have been plated in a 96 well plate the day before. After 24 hours of exposition, cell viability was evaluated through the Crystal Violet assay. Absorvance was measured at 595nm in a microplate reader (Biorad 680). The results were evaluated by Student’s t-test (SPSS, Chicago, IL) and the level of significance was chosen as p-value <0.05.Results: Undiluted extracts of both resins produce significant differences in mouse fibroblasts viability when they were incubated for 14 days (Figure 1). Even though, with all dilutions and incubation times of the extracts tested, cell viability was never below 70%, which is the threshold stated in ISO 10993-5:2009 to be considered cytotoxic [3].Open in a separate windowFigure 1.Cell viability of 3T3 fibroblasts when exposed to ProBase® resins. The values are present as mean ± SD in relation to non-exposed cells (defined as 100% cell viability). *p < 0.05 when compared to non-exposed cells." @default.
- W2946916693 created "2019-06-07" @default.
- W2946916693 creator A5009815421 @default.
- W2946916693 creator A5010047291 @default.
- W2946916693 creator A5015956023 @default.
- W2946916693 creator A5023365342 @default.
- W2946916693 creator A5041881720 @default.
- W2946916693 creator A5046191305 @default.
- W2946916693 creator A5047148494 @default.
- W2946916693 creator A5067042190 @default.
- W2946916693 date "2019-03-29" @default.
- W2946916693 modified "2023-10-15" @default.
- W2946916693 title "Effect of polymerization methods in the cytotoxicity of two resins used in removable prosthodontics – an in vitro study" @default.
- W2946916693 cites W2246163271 @default.
- W2946916693 doi "https://doi.org/10.1080/07853890.2018.1561990" @default.
- W2946916693 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/7888734" @default.
- W2946916693 hasPublicationYear "2019" @default.
- W2946916693 type Work @default.
- W2946916693 sameAs 2946916693 @default.
- W2946916693 citedByCount "0" @default.
- W2946916693 crossrefType "journal-article" @default.
- W2946916693 hasAuthorship W2946916693A5009815421 @default.
- W2946916693 hasAuthorship W2946916693A5010047291 @default.
- W2946916693 hasAuthorship W2946916693A5015956023 @default.
- W2946916693 hasAuthorship W2946916693A5023365342 @default.
- W2946916693 hasAuthorship W2946916693A5041881720 @default.
- W2946916693 hasAuthorship W2946916693A5046191305 @default.
- W2946916693 hasAuthorship W2946916693A5047148494 @default.
- W2946916693 hasAuthorship W2946916693A5067042190 @default.
- W2946916693 hasBestOaLocation W29469166931 @default.
- W2946916693 hasConcept C109316439 @default.
- W2946916693 hasConcept C136229726 @default.
- W2946916693 hasConcept C142724271 @default.
- W2946916693 hasConcept C178790620 @default.
- W2946916693 hasConcept C184112429 @default.
- W2946916693 hasConcept C185592680 @default.
- W2946916693 hasConcept C192562407 @default.
- W2946916693 hasConcept C199343813 @default.
- W2946916693 hasConcept C202751555 @default.
- W2946916693 hasConcept C204787440 @default.
- W2946916693 hasConcept C2780783641 @default.
- W2946916693 hasConcept C44228677 @default.
- W2946916693 hasConcept C521977710 @default.
- W2946916693 hasConcept C53227056 @default.
- W2946916693 hasConcept C55493867 @default.
- W2946916693 hasConcept C71924100 @default.
- W2946916693 hasConceptScore W2946916693C109316439 @default.
- W2946916693 hasConceptScore W2946916693C136229726 @default.
- W2946916693 hasConceptScore W2946916693C142724271 @default.
- W2946916693 hasConceptScore W2946916693C178790620 @default.
- W2946916693 hasConceptScore W2946916693C184112429 @default.
- W2946916693 hasConceptScore W2946916693C185592680 @default.
- W2946916693 hasConceptScore W2946916693C192562407 @default.
- W2946916693 hasConceptScore W2946916693C199343813 @default.
- W2946916693 hasConceptScore W2946916693C202751555 @default.
- W2946916693 hasConceptScore W2946916693C204787440 @default.
- W2946916693 hasConceptScore W2946916693C2780783641 @default.
- W2946916693 hasConceptScore W2946916693C44228677 @default.
- W2946916693 hasConceptScore W2946916693C521977710 @default.
- W2946916693 hasConceptScore W2946916693C53227056 @default.
- W2946916693 hasConceptScore W2946916693C55493867 @default.
- W2946916693 hasConceptScore W2946916693C71924100 @default.
- W2946916693 hasLocation W29469166931 @default.
- W2946916693 hasLocation W29469166932 @default.
- W2946916693 hasOpenAccess W2946916693 @default.
- W2946916693 hasPrimaryLocation W29469166931 @default.
- W2946916693 hasRelatedWork W1969123000 @default.
- W2946916693 hasRelatedWork W1983495937 @default.
- W2946916693 hasRelatedWork W2039963225 @default.
- W2946916693 hasRelatedWork W2048264597 @default.
- W2946916693 hasRelatedWork W2051721150 @default.
- W2946916693 hasRelatedWork W2093216206 @default.
- W2946916693 hasRelatedWork W2110974459 @default.
- W2946916693 hasRelatedWork W2163685863 @default.
- W2946916693 hasRelatedWork W2170068805 @default.
- W2946916693 hasRelatedWork W2261895806 @default.
- W2946916693 hasRelatedWork W2384965737 @default.
- W2946916693 hasRelatedWork W2386495488 @default.
- W2946916693 hasRelatedWork W2416987931 @default.
- W2946916693 hasRelatedWork W2510403824 @default.
- W2946916693 hasRelatedWork W2559957672 @default.
- W2946916693 hasRelatedWork W2609212624 @default.
- W2946916693 hasRelatedWork W2738333523 @default.
- W2946916693 hasRelatedWork W2998117740 @default.
- W2946916693 hasRelatedWork W3032923459 @default.
- W2946916693 hasRelatedWork W3156540963 @default.
- W2946916693 isParatext "false" @default.
- W2946916693 isRetracted "false" @default.
- W2946916693 magId "2946916693" @default.
- W2946916693 workType "article" @default.