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- W2947100838 abstract "Dabai, Canarium odontophyllum Miq. an indigenous fruit tree of Sarawak is dioecious, having male andhermaphrodite trees. Vegetative propagation of by conventional methods though not highly successful iscurrently the only mean to produce clones of selected genotypes. Micropropagation has been considered as analternative to multiply the selected clones of Dabai. In this approach, an effective protocol for establishmentof contamination-free and viable explants is a prerequisite for regeneration of plant in vitro. This study aimedprimarily to improve a protocol developed earlier on establishment of contamination-free and viable cultureand then to attempt plant regeneration through proliferation of axillary shoots, organogenesis or somaticembryogenesis. A protocol which can achieve two fold increases in the percentage of contamination-freeculture has been developed. The protocol involved spraying of 75% (v/v) Ethanol to stock plants prior tocollection of explants which were then surface sterilized for 20 minutes in 20% (v/v) Clorox and thenagitated in 0.3% (w/v) Benomyl for one hour before cultured in solid half-strength MS media supplementedwith 3 mg/L BAP, 2 ml/L PPM, and 10 mg/L TET. Induction of direct organogenesis has been initiated recently. To date only roots were observed developing from young cotyledon explants. Callus had been induced from the cotyledons in 3.0-9.0 mg/L 2,4-D in the attempt on induction of somatic embryogenesis. In conclusion, 80% contamination-free culture could be achieved using that protocol. Extension of axillary shoot growth and root formation on cotyledon explants could be observed." @default.
- W2947100838 created "2019-06-07" @default.
- W2947100838 creator A5045752036 @default.
- W2947100838 date "2009-01-01" @default.
- W2947100838 modified "2023-09-24" @default.
- W2947100838 title "Establishment of Contamination-free Culture and Induction of In Vitro Plant Regeneration in Dabai (Canarium odontophyllum Miq.)" @default.
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