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• W2947203488 abstract "Engineering microbial biosynthetic pathways represents a compelling route to gain access to expanded chemical diversity. Carrier proteins (CPs) play a central role in biosynthesis, but the fast motions of CPs make their conformational dynamics difficult to capture using traditional spectroscopic approaches. Here we present a low-resource method to directly reveal carrier protein-substrate interactions. Chemoenzymatic loading of commercially available, alkyne-containing substrates onto CPs enables rapid visualization of the molecular cargo's local environment using Raman spectroscopy. This method could clarify the foundations of the chain sequestration mechanism, facilitate the rapid characterization of CPs, and enable visualization of the vectoral processing of natural products both in vitro and in vivo." @default.
• W2947203488 created "2019-06-07" @default.
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• W2947203488 date "2019-05-20" @default.
• W2947203488 modified "2023-10-13" @default.
• W2947203488 title "Tracking carrier protein motions with Raman spectroscopy" @default.
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• W2947203488 doi "https://doi.org/10.1038/s41467-019-10184-2" @default.
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• W2947203488 hasPublicationYear "2019" @default.
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