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- W2947239088 abstract "Apheresis in children with low body weight is technically limited by their tolerance of the extracorporeal blood volume.This paper presents a single-center experience with 23 procedures in 12 children with weights between 5.2 and 9.5 kg using the Spectra Optia mononuclear cell (MNC) protocol with blood priming.The average procedure duration was 158 minutes, and the median processed blood volume was 316 mL/kg. The white blood cell (WBC), platelet (PLT), and hemoglobin (HGB) values showed a downward trend with increased volume of processed blood. The post-apheresis HGB concentration was increased in all procedures due to initial priming with packed red blood cells (PRBCs), but this effect disappeared at a level of ~400 mL of processed blood/kg. The median volume of the cellular product was 36 mL, the WBC count was 153 K/μL, the hematocrit (HCT) was 1.5%, the PLT count was 602 K/μL, the WBC collection efficacy (CE2) was 13.2%, and the PLT CE2 was 9.5%. The median CD34+ CE2 was 28%, and interpolation of the CD34+ CE2 yielded a Y-intercept value of 32%. Higher pre-collection CD34+ counts resulted in higher CD34+ yields. No correlation was found between the pre-collection CD34+ results and CD34+ CE2.The analyzed data demonstrated the feasibility and safety of apheresis in very low-weight children. The laboratory abnormalities were asymptomatic and citrate toxicity was mild. Visual control of clogging with manual adjustment of the citrate infusion rate is important to reduce exposure to citrate." @default.
- W2947239088 created "2019-06-07" @default.
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- W2947239088 date "2019-05-29" @default.
- W2947239088 modified "2023-10-13" @default.
- W2947239088 title "Safety and efficacy of autologous mononuclear cell and stem cell apheresis in very low‐weight children—Experience at a single center" @default.
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- W2947239088 doi "https://doi.org/10.1002/jca.21713" @default.
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