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• W2947366003 abstract "Murine models are valuable tools to study the role of gut microbiota in health or disease. However, murine and human microbiota differ in species composition, so further investigation of the murine gut microbiota is important to gain a better mechanistic understanding. Continuous in vitro fermentation models are powerful tools to investigate microbe-microbe interactions while circumventing animal testing and host confounding factors, but are lacking for murine gut microbiota. We therefore developed a novel continuous fermentation model based on the PolyFermS platform adapted to the murine caecum and inoculated with immobilized caecal microbiota. We followed a stepwise model development approach by adjusting parameters (pH, retention time, growth medium) to reach fermentation metabolite profiles and marker bacterial levels similar to the inoculum. The final model had a stable and inoculum-alike fermentation profile during continuous operation. A lower pH during startup and continuous operation stimulated bacterial fermentation (115 mM short-chain fatty acids at pH 7 to 159 mM at pH 6.5). Adjustments to nutritive medium, a decreased pH and increased retention time helped control the in vitro Enterobacteriaceae levels, which often bloom in fermentation models, to 6.6 log gene copies/mL in final model. In parallel, the Lactobacillus, Lachnospiraceae, and Ruminococcaceae levels were better maintained in vitro with concentrations of 8.5 log gene copies/mL, 8.8 log gene copies/mL and 7.5 log gene copies/mL, respectively, in the final model. An independent repetition with final model parameters showed reproducible results in maintaining the inoculum fermentation metabolite profile and its marker bacterial levels. Microbiota community analysis of the final model showed a decreased bacterial diversity and compositional differences compared to caecal inoculum microbiota. Most of the caecal bacterial families were represented in vitro, but taxa of the Muribaculaceae family were not maintained. Functional metagenomics prediction showed conserved metabolic and functional KEGG pathways between in vitro and caecal inoculum microbiota. To conclude, we showed that a rational and stepwise approach allowed us to model in vitro the murine caecal microbiota and functions. Our model is a first step to develop murine microbiota model systems and offers the potential to study microbiota functionality and structure ex vivo." @default.
• W2947366003 created "2019-06-07" @default.
• W2947366003 creator A5007856392 @default.
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• W2947366003 date "2019-05-29" @default.
• W2947366003 modified "2023-10-11" @default.
• W2947366003 title "Stepwise Development of an in vitro Continuous Fermentation Model for the Murine Caecal Microbiota" @default.
• W2947366003 cites W1480028928 @default.
• W2947366003 cites W1507882192 @default.
• W2947366003 cites W1712483442 @default.
• W2947366003 cites W1716352502 @default.
• W2947366003 cites W1805101691 @default.
• W2947366003 cites W1846732964 @default.
• W2947366003 cites W1915524531 @default.
• W2947366003 cites W1970109094 @default.
• W2947366003 cites W1971312144 @default.
• W2947366003 cites W1971415551 @default.
• W2947366003 cites W1980200453 @default.
• W2947366003 cites W1994227600 @default.
• W2947366003 cites W2003808681 @default.
• W2947366003 cites W2004014148 @default.
• W2947366003 cites W2005741884 @default.
• W2947366003 cites W2008549364 @default.
• W2947366003 cites W2016539998 @default.
• W2947366003 cites W2020621701 @default.
• W2947366003 cites W2034082915 @default.
• W2947366003 cites W2041032345 @default.
• W2947366003 cites W2041248859 @default.
• W2947366003 cites W2044557931 @default.
• W2947366003 cites W2045073446 @default.
• W2947366003 cites W2048853985 @default.
• W2947366003 cites W2062333123 @default.
• W2947366003 cites W2063577643 @default.
• W2947366003 cites W2070807248 @default.
• W2947366003 cites W2072970694 @default.
• W2947366003 cites W2081653606 @default.
• W2947366003 cites W2084796563 @default.
• W2947366003 cites W2088833470 @default.
• W2947366003 cites W2090925778 @default.
• W2947366003 cites W2098762803 @default.
• W2947366003 cites W2105831375 @default.
• W2947366003 cites W2110300022 @default.
• W2947366003 cites W2111317067 @default.
• W2947366003 cites W2116726801 @default.
• W2947366003 cites W2117595393 @default.
• W2947366003 cites W2119914001 @default.
• W2947366003 cites W2121939102 @default.
• W2947366003 cites W2127997386 @default.
• W2947366003 cites W2141300346 @default.
• W2947366003 cites W2148103005 @default.
• W2947366003 cites W2148570593 @default.
• W2947366003 cites W2160427027 @default.
• W2947366003 cites W2160727103 @default.
• W2947366003 cites W2162913040 @default.
• W2947366003 cites W2163878401 @default.
• W2947366003 cites W2170744053 @default.
• W2947366003 cites W2173894147 @default.
• W2947366003 cites W2179438025 @default.
• W2947366003 cites W2197368019 @default.
• W2947366003 cites W2236332835 @default.
• W2947366003 cites W2271650201 @default.
• W2947366003 cites W2301371409 @default.
• W2947366003 cites W2312126710 @default.
• W2947366003 cites W2334391268 @default.
• W2947366003 cites W2336254837 @default.
• W2947366003 cites W2339476146 @default.
• W2947366003 cites W2462759270 @default.
• W2947366003 cites W2473762402 @default.
• W2947366003 cites W2474000574 @default.
• W2947366003 cites W2517988427 @default.
• W2947366003 cites W2526966274 @default.
• W2947366003 cites W2549269050 @default.
• W2947366003 cites W2550084345 @default.
• W2947366003 cites W2553615018 @default.
• W2947366003 cites W2590245531 @default.
• W2947366003 cites W2605173654 @default.
• W2947366003 cites W2627013908 @default.
• W2947366003 cites W2738379602 @default.
• W2947366003 cites W2752092519 @default.
• W2947366003 cites W2767066299 @default.
• W2947366003 cites W2767590364 @default.
• W2947366003 cites W2770744386 @default.
• W2947366003 cites W2785553342 @default.
• W2947366003 cites W2790225783 @default.
• W2947366003 cites W2791035781 @default.
• W2947366003 cites W2801107067 @default.
• W2947366003 cites W2808263118 @default.
• W2947366003 cites W797998171 @default.
• W2947366003 doi "https://doi.org/10.3389/fmicb.2019.01166" @default.
• W2947366003 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6548829" @default.
• W2947366003 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/31191488" @default.
• W2947366003 hasPublicationYear "2019" @default.
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