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- W2947376575 abstract "Hydroxytyrosol is a high-value-added compound with a variety of biological and pharmacological activities. In this study, a whole-cell catalytic method for the synthesis of hydroxytyrosol was developed: aromatic amino acid aminotransferase (TyrB), l-glutamate dehydrogenase (GDH), α-keto acid decarboxylase (PmKDC), and aldehyde reductase (YahK) were co-expressed in Escherichia coli to catalyze the synthesis of hydroxytyrosol from l-3,4-dihydroxyphenylalanine (l-DOPA). The plasmids with different copy numbers were used to balance the expression of the four enzymes, and the most appropriate strain (pRSF-yahK-tyrB and pCDF-gdh-Pmkdc) was identified. After determination of the optimum temperature (35 °C) and pH (7.5) for whole-cell catalysis, the yield of hydroxytyrosol reached 36.33 mM (5.59 g/L) and the space-time yield reached 0.70 g L–1 h–1." @default.
- W2947376575 created "2019-06-07" @default.
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- W2947376575 date "2019-05-28" @default.
- W2947376575 modified "2023-10-09" @default.
- W2947376575 title "Efficient Synthesis of Hydroxytyrosol from <scp>l</scp>-3,4-Dihydroxyphenylalanine Using Engineered <i>Escherichia coli</i> Whole Cells" @default.
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- W2947376575 doi "https://doi.org/10.1021/acs.jafc.9b01856" @default.
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