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- W2947432633 abstract "Powdery mildew is caused by Podosphaera xanthii, and is one of the most important diseases that attacks Spanish cucurbit crops. Fungicide application is the primary control tool; however, its effectiveness is hampered by the rapid development of resistance to these compounds. In this study, the EC50 values of 26 isolates were determined in response to the succinate dehydrogenase inhibitor (SDHI) fungicides boscalid and fluopyram. From these data, the discriminatory doses were deduced and used for SDHI resistance monitoring during the 2018 and 2019 growing seasons. Of the 298 isolates analysed, 37.9% showed resistance to boscalid and 44% to fluopyram. Although different phenotypes were observed in leaf disc assays, the resistant isolates showed the same phenotype in plant assays. Compared to sensitive isolates, two amino acid changes were found in the SdhC subunit, A86V and G151R, which are associated mostly with resistance patterns to fluopyram and boscalid, respectively. Furthermore, no significant differences were observed in terms of fitness cost between the selected sensitive and resistant isolates analysed here. Lastly, a loop-mediated isothermal amplification (LAMP) assay was developed to detect A86V and G151R mutations using conidia obtained directly from infected material. Our results show that growers could continue to use boscalid and fluopyram, but resistance management practices must be implemented." @default.
- W2947432633 created "2019-06-07" @default.
- W2947432633 creator A5022725306 @default.
- W2947432633 creator A5036920205 @default.
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- W2947432633 creator A5066993860 @default.
- W2947432633 creator A5067720147 @default.
- W2947432633 creator A5086909935 @default.
- W2947432633 date "2021-09-08" @default.
- W2947432633 modified "2023-10-18" @default.
- W2947432633 title "Resistance to the SDHI Fungicides Boscalid and Fluopyram in Podosphaera xanthii Populations from Commercial Cucurbit Fields in Spain" @default.
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- W2947432633 doi "https://doi.org/10.3390/jof7090733" @default.
- W2947432633 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/8464660" @default.
- W2947432633 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/34575771" @default.
- W2947432633 hasPublicationYear "2021" @default.
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