FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2947451738> ?p ?o ?g. }

• W2947451738 endingPage "70" @default.
• W2947451738 startingPage "70" @default.
• W2947451738 abstract "Bacteriophage endolysins, enzymes that degrade the bacterial peptidoglycan (PG), have gained an increasing interest as alternative antimicrobial agents, due to their ability to kill antibiotic resistant pathogens efficiently when applied externally as purified proteins. Typical endolysins derived from bacteriophage that infect Gram-positive hosts consist of an N-terminal enzymatically-active domain (EAD) that cleaves covalent bonds in the PG, and a C-terminal cell-binding domain (CBD) that recognizes specific ligands on the surface of the PG. Although CBDs are usually essential for the EADs to access the PG substrate, some EADs possess activity in the absence of CBDs, and a few even display better activity profiles or an extended host spectrum than the full-length endolysin. A current hypothesis suggests a net positive charge on the EAD enables it to reach the negatively charged bacterial surface via ionic interactions in the absence of a CBD. Here, we used the PlyC CHAP domain as a model EAD to further test the hypothesis. We mutated negatively charged surface amino acids of the CHAP domain that are not involved in structured regions to neutral or positively charged amino acids in order to increase the net charge from -3 to a range from +1 to +7. The seven mutant candidates were successfully expressed and purified as soluble proteins. Contrary to the current hypothesis, none of the mutants were more active than wild-type CHAP. Analysis of electrostatic surface potential implies that the surface charge distribution may affect the activity of a positively charged EAD. Thus, we suggest that while charge should continue to be considered for future engineering efforts, it should not be the sole focus of such engineering efforts." @default.
• W2947451738 created "2019-06-07" @default.
• W2947451738 creator A5070975898 @default.
• W2947451738 creator A5075146072 @default.
• W2947451738 date "2019-05-28" @default.
• W2947451738 modified "2023-10-03" @default.
• W2947451738 title "Contributions of Net Charge on the PlyC Endolysin CHAP Domain" @default.
• W2947451738 cites W1852844868 @default.
• W2947451738 cites W1892158199 @default.
• W2947451738 cites W1964376584 @default.
• W2947451738 cites W1966313166 @default.
• W2947451738 cites W1974209877 @default.
• W2947451738 cites W1980590015 @default.
• W2947451738 cites W1981232704 @default.
• W2947451738 cites W2006793281 @default.
• W2947451738 cites W2007806539 @default.
• W2947451738 cites W2009434802 @default.
• W2947451738 cites W2013598528 @default.
• W2947451738 cites W2023490488 @default.
• W2947451738 cites W2026292570 @default.
• W2947451738 cites W2042217887 @default.
• W2947451738 cites W2054257072 @default.
• W2947451738 cites W2063206735 @default.
• W2947451738 cites W2064125274 @default.
• W2947451738 cites W2069018460 @default.
• W2947451738 cites W2086910699 @default.
• W2947451738 cites W2090627629 @default.
• W2947451738 cites W2092387745 @default.
• W2947451738 cites W2096326200 @default.
• W2947451738 cites W2109861005 @default.
• W2947451738 cites W2119225291 @default.
• W2947451738 cites W2153843103 @default.
• W2947451738 cites W2154066176 @default.
• W2947451738 cites W2171986805 @default.
• W2947451738 cites W2172074380 @default.
• W2947451738 cites W2201713963 @default.
• W2947451738 cites W2413053666 @default.
• W2947451738 cites W2502683607 @default.
• W2947451738 cites W2531618678 @default.
• W2947451738 cites W2537863070 @default.
• W2947451738 cites W2771057811 @default.
• W2947451738 cites W2890261355 @default.
• W2947451738 cites W2908926838 @default.
• W2947451738 cites W4019147 @default.
• W2947451738 doi "https://doi.org/10.3390/antibiotics8020070" @default.
• W2947451738 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6628322" @default.
• W2947451738 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/31142020" @default.
• W2947451738 hasPublicationYear "2019" @default.
• W2947451738 type Work @default.
• W2947451738 sameAs 2947451738 @default.
• W2947451738 citedByCount "9" @default.
• W2947451738 countsByYear W29474517382019 @default.
• W2947451738 countsByYear W29474517382020 @default.
• W2947451738 countsByYear W29474517382021 @default.
• W2947451738 countsByYear W29474517382022 @default.
• W2947451738 countsByYear W29474517382023 @default.
• W2947451738 crossrefType "journal-article" @default.
• W2947451738 hasAuthorship W2947451738A5070975898 @default.
• W2947451738 hasAuthorship W2947451738A5075146072 @default.
• W2947451738 hasBestOaLocation W29474517381 @default.
• W2947451738 hasConcept C104317684 @default.
• W2947451738 hasConcept C12554922 @default.
• W2947451738 hasConcept C143065580 @default.
• W2947451738 hasConcept C147789679 @default.
• W2947451738 hasConcept C181199279 @default.
• W2947451738 hasConcept C185592680 @default.
• W2947451738 hasConcept C22538091 @default.
• W2947451738 hasConcept C2776441376 @default.
• W2947451738 hasConcept C2776634448 @default.
• W2947451738 hasConcept C2780358262 @default.
• W2947451738 hasConcept C515207424 @default.
• W2947451738 hasConcept C547475151 @default.
• W2947451738 hasConcept C55493867 @default.
• W2947451738 hasConcept C86803240 @default.
• W2947451738 hasConceptScore W2947451738C104317684 @default.
• W2947451738 hasConceptScore W2947451738C12554922 @default.
• W2947451738 hasConceptScore W2947451738C143065580 @default.
• W2947451738 hasConceptScore W2947451738C147789679 @default.
• W2947451738 hasConceptScore W2947451738C181199279 @default.
• W2947451738 hasConceptScore W2947451738C185592680 @default.
• W2947451738 hasConceptScore W2947451738C22538091 @default.
• W2947451738 hasConceptScore W2947451738C2776441376 @default.
• W2947451738 hasConceptScore W2947451738C2776634448 @default.
• W2947451738 hasConceptScore W2947451738C2780358262 @default.
• W2947451738 hasConceptScore W2947451738C515207424 @default.
• W2947451738 hasConceptScore W2947451738C547475151 @default.
• W2947451738 hasConceptScore W2947451738C55493867 @default.
• W2947451738 hasConceptScore W2947451738C86803240 @default.
• W2947451738 hasFunder F4320332299 @default.
• W2947451738 hasIssue "2" @default.
• W2947451738 hasLocation W29474517381 @default.
• W2947451738 hasLocation W29474517382 @default.
• W2947451738 hasLocation W29474517383 @default.
• W2947451738 hasLocation W29474517384 @default.
• W2947451738 hasOpenAccess W2947451738 @default.
• W2947451738 hasPrimaryLocation W29474517381 @default.
• W2947451738 hasRelatedWork W1864887623 @default.
• W2947451738 hasRelatedWork W1890692049 @default.

• next