FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2947557968> ?p ?o ?g. }

• W2947557968 abstract "Riemerella anatipestifer (R. anatipestifer) is one of the most important poultry pathogens worldwide, with associated infections causing significant economic losses. Rifampin Resistance is an important mechanism of drug resistance. However, there is no information about rpoB mutations conferring rifampin resistance and its fitness cost in Riemerella anatipestifer. Comparative analysis of 18 R.anatipestifer rpoB sequences and the determination of rifampin minimum inhibitory concentrations showed that five point mutations, V382I, H491N, G502K, R494K and S539Y, were related to rifampin resistance. Five overexpression strains were constructed using site-directed mutagenesis to validate these sites. To investigate the origin and fitness costs of the rpoB mutations, 15 types of rpoB mutations were isolated from R. anatipestifer ATCC 11845 by using spontaneous mutation in which R494K was identical to the type of mutation detected in the isolates. The mutation frequency of the rpoB gene was calculated to be 10− 8. A total of 98.8% (247/250) of the obtained mutants were located in cluster I of the rifampin resistance-determining region of the rpoB gene. With the exception of D481Y, I537N and S539F, the rifampin minimum inhibitory concentrations of the remaining mutants were at least 64 μg/mL. The growth performance and competitive experiments of the mutant strains in vitro showed that H491D and 485::TAA exhibit growth delay and severely impaired fitness. Finally, the colonization abilities and sensitivities of the R494K and H491D mutants were investigated. The sensitivity of the two mutants to hydrogen peroxide (H2O2) and sodium nitroprusside (SNP) increased compared to the parental strain. The number of live colonies colonized by the two mutants in the duckling brain and trachea were lower than that of the parental strain within 24 h. Mutations of rpoB gene in R. anatipestifer mediate rifampin resistance and result in fitness costs. And different single mutations confer different levels of fitness costs. Our study provides, to our knowledge, the first estimates of the fitness cost associated with the R. anatipestifer rifampin resistance in vitro and in vivo." @default.
• W2947557968 created "2019-06-07" @default.
• W2947557968 creator A5007648400 @default.
• W2947557968 creator A5007933016 @default.
• W2947557968 creator A5013454053 @default.
• W2947557968 creator A5021517739 @default.
• W2947557968 creator A5028136131 @default.
• W2947557968 creator A5031331731 @default.
• W2947557968 creator A5039291898 @default.
• W2947557968 creator A5044399300 @default.
• W2947557968 creator A5047307954 @default.
• W2947557968 creator A5049672306 @default.
• W2947557968 creator A5064308879 @default.
• W2947557968 creator A5068138665 @default.
• W2947557968 creator A5076996063 @default.
• W2947557968 creator A5088124858 @default.
• W2947557968 creator A5090209124 @default.
• W2947557968 creator A5090219584 @default.
• W2947557968 date "2019-05-23" @default.
• W2947557968 modified "2023-10-16" @default.
• W2947557968 title "Rifampin resistance and its fitness cost in Riemerella anatipestifer" @default.
• W2947557968 cites W1674828992 @default.
• W2947557968 cites W1835214804 @default.
• W2947557968 cites W1978061829 @default.
• W2947557968 cites W2002412136 @default.
• W2947557968 cites W2004169172 @default.
• W2947557968 cites W2004254509 @default.
• W2947557968 cites W2018823905 @default.
• W2947557968 cites W2038211892 @default.
• W2947557968 cites W2043876589 @default.
• W2947557968 cites W2054902612 @default.
• W2947557968 cites W2063563543 @default.
• W2947557968 cites W2068148879 @default.
• W2947557968 cites W2068372433 @default.
• W2947557968 cites W2090640084 @default.
• W2947557968 cites W2092416350 @default.
• W2947557968 cites W2104728958 @default.
• W2947557968 cites W2105262308 @default.
• W2947557968 cites W2112510426 @default.
• W2947557968 cites W2115263303 @default.
• W2947557968 cites W2135484028 @default.
• W2947557968 cites W2138332261 @default.
• W2947557968 cites W2147806507 @default.
• W2947557968 cites W2149006494 @default.
• W2947557968 cites W2167988552 @default.
• W2947557968 cites W2169686765 @default.
• W2947557968 cites W2228739457 @default.
• W2947557968 cites W2248582126 @default.
• W2947557968 cites W2287444413 @default.
• W2947557968 cites W2386571266 @default.
• W2947557968 cites W2401298942 @default.
• W2947557968 cites W2554704373 @default.
• W2947557968 cites W2592850816 @default.
• W2947557968 cites W2594849308 @default.
• W2947557968 cites W2616035838 @default.
• W2947557968 cites W2745555721 @default.
• W2947557968 cites W2771402697 @default.
• W2947557968 cites W2791493789 @default.
• W2947557968 cites W2023456501 @default.
• W2947557968 doi "https://doi.org/10.1186/s12866-019-1478-7" @default.
• W2947557968 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6533769" @default.
• W2947557968 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/31122209" @default.
• W2947557968 hasPublicationYear "2019" @default.
• W2947557968 type Work @default.
• W2947557968 sameAs 2947557968 @default.
• W2947557968 citedByCount "11" @default.
• W2947557968 countsByYear W29475579682020 @default.
• W2947557968 countsByYear W29475579682021 @default.
• W2947557968 countsByYear W29475579682022 @default.
• W2947557968 countsByYear W29475579682023 @default.
• W2947557968 crossrefType "journal-article" @default.
• W2947557968 hasAuthorship W2947557968A5007648400 @default.
• W2947557968 hasAuthorship W2947557968A5007933016 @default.
• W2947557968 hasAuthorship W2947557968A5013454053 @default.
• W2947557968 hasAuthorship W2947557968A5021517739 @default.
• W2947557968 hasAuthorship W2947557968A5028136131 @default.
• W2947557968 hasAuthorship W2947557968A5031331731 @default.
• W2947557968 hasAuthorship W2947557968A5039291898 @default.
• W2947557968 hasAuthorship W2947557968A5044399300 @default.
• W2947557968 hasAuthorship W2947557968A5047307954 @default.
• W2947557968 hasAuthorship W2947557968A5049672306 @default.
• W2947557968 hasAuthorship W2947557968A5064308879 @default.
• W2947557968 hasAuthorship W2947557968A5068138665 @default.
• W2947557968 hasAuthorship W2947557968A5076996063 @default.
• W2947557968 hasAuthorship W2947557968A5088124858 @default.
• W2947557968 hasAuthorship W2947557968A5090209124 @default.
• W2947557968 hasAuthorship W2947557968A5090219584 @default.
• W2947557968 hasBestOaLocation W29475579681 @default.
• W2947557968 hasConcept C104317684 @default.
• W2947557968 hasConcept C143065580 @default.
• W2947557968 hasConcept C2778340795 @default.
• W2947557968 hasConcept C42062724 @default.
• W2947557968 hasConcept C501734568 @default.
• W2947557968 hasConcept C54355233 @default.
• W2947557968 hasConcept C86803240 @default.
• W2947557968 hasConcept C89423630 @default.
• W2947557968 hasConceptScore W2947557968C104317684 @default.
• W2947557968 hasConceptScore W2947557968C143065580 @default.
• W2947557968 hasConceptScore W2947557968C2778340795 @default.
• W2947557968 hasConceptScore W2947557968C42062724 @default.

• next