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- W2947572421 abstract "Recently, plants have emerged as a lucrative alternative system for the production of recombinant proteins, as recombinant proteins produced in plants are safer and cheaper than those produced in bacteria and animal cell-based production systems. To obtain high yields in plants, recombinant proteins are produced in chloroplasts using different strategies. The first strategy is based on chloroplast transformation, followed by gene expression and translation in chloroplasts. This has proven to be a powerful approach for the production of proteins at high levels. The second approach is based on nuclear transformation, followed by post-translational import of proteins from the cytosol into chloroplasts. In the nuclear transformation approach, foreign genes are stably integrated into the nuclear genome or transiently expressed in the nucleus by non-integrating T-DNA. Although this approach also has great potential for protein production at high levels, it has not been thoroughly investigated. In this review, we focus on nuclear transformation-based protein expression and its subsequent sequestration in chloroplasts, and summarize the different strategies used for high-level production of recombinant proteins. We also discuss future directions for further improvements in protein production in chloroplasts through nuclear transformation-based gene expression." @default.
- W2947572421 created "2019-06-07" @default.
- W2947572421 creator A5015797506 @default.
- W2947572421 creator A5032300598 @default.
- W2947572421 creator A5080970990 @default.
- W2947572421 creator A5083020137 @default.
- W2947572421 date "2019-05-28" @default.
- W2947572421 modified "2023-09-26" @default.
- W2947572421 title "Production of recombinant proteins through sequestration in chloroplasts: a strategy based on nuclear transformation and post-translational protein import" @default.
- W2947572421 cites W1535327658 @default.
- W2947572421 cites W1969932115 @default.
- W2947572421 cites W1970468139 @default.
- W2947572421 cites W1971308430 @default.
- W2947572421 cites W1973060237 @default.
- W2947572421 cites W1974767167 @default.
- W2947572421 cites W1974877324 @default.
- W2947572421 cites W1981069257 @default.
- W2947572421 cites W1982197198 @default.
- W2947572421 cites W1993055801 @default.
- W2947572421 cites W1999669751 @default.
- W2947572421 cites W2002062036 @default.
- W2947572421 cites W2005330292 @default.
- W2947572421 cites W2005911581 @default.
- W2947572421 cites W2009087758 @default.
- W2947572421 cites W2014083195 @default.
- W2947572421 cites W2015414884 @default.
- W2947572421 cites W2015642880 @default.
- W2947572421 cites W2022567869 @default.
- W2947572421 cites W2024482376 @default.
- W2947572421 cites W2027894892 @default.
- W2947572421 cites W2030101006 @default.
- W2947572421 cites W2033488705 @default.
- W2947572421 cites W2034845518 @default.
- W2947572421 cites W2042176753 @default.
- W2947572421 cites W2044794038 @default.
- W2947572421 cites W2046182783 @default.
- W2947572421 cites W2046347466 @default.
- W2947572421 cites W2046739387 @default.
- W2947572421 cites W2052407981 @default.
- W2947572421 cites W2061156822 @default.
- W2947572421 cites W2063745545 @default.
- W2947572421 cites W2064954917 @default.
- W2947572421 cites W2065035248 @default.
- W2947572421 cites W2067067694 @default.
- W2947572421 cites W2075536499 @default.
- W2947572421 cites W2084264866 @default.
- W2947572421 cites W2088076755 @default.
- W2947572421 cites W2091800801 @default.
- W2947572421 cites W2095596057 @default.
- W2947572421 cites W2095854628 @default.
- W2947572421 cites W2099365403 @default.
- W2947572421 cites W2103456166 @default.
- W2947572421 cites W2107648210 @default.
- W2947572421 cites W2112672091 @default.
- W2947572421 cites W2113208618 @default.
- W2947572421 cites W2113881961 @default.
- W2947572421 cites W2115280743 @default.
- W2947572421 cites W2119390255 @default.
- W2947572421 cites W2120609288 @default.
- W2947572421 cites W2124280483 @default.
- W2947572421 cites W2128966805 @default.
- W2947572421 cites W2129095580 @default.
- W2947572421 cites W2129113294 @default.
- W2947572421 cites W2134884592 @default.
- W2947572421 cites W2136309050 @default.
- W2947572421 cites W2137541144 @default.
- W2947572421 cites W2140617473 @default.
- W2947572421 cites W2141052107 @default.
- W2947572421 cites W2158732170 @default.
- W2947572421 cites W2162031682 @default.
- W2947572421 cites W2164321625 @default.
- W2947572421 cites W2171897170 @default.
- W2947572421 cites W2172195350 @default.
- W2947572421 cites W2176043983 @default.
- W2947572421 cites W2299652140 @default.
- W2947572421 cites W2414420506 @default.
- W2947572421 cites W2475158195 @default.
- W2947572421 cites W2517366254 @default.
- W2947572421 cites W2605489416 @default.
- W2947572421 cites W2606159141 @default.
- W2947572421 cites W2739276987 @default.
- W2947572421 cites W2745110995 @default.
- W2947572421 cites W2766370739 @default.
- W2947572421 cites W2768119869 @default.
- W2947572421 cites W2768520749 @default.
- W2947572421 cites W2770948545 @default.
- W2947572421 cites W2792031579 @default.
- W2947572421 cites W2796214670 @default.
- W2947572421 cites W2884152980 @default.
- W2947572421 cites W2901043781 @default.
- W2947572421 cites W2905705045 @default.
- W2947572421 cites W2919736772 @default.
- W2947572421 cites W307436637 @default.
- W2947572421 doi "https://doi.org/10.1007/s00299-019-02431-z" @default.
- W2947572421 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/31139894" @default.
- W2947572421 hasPublicationYear "2019" @default.
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