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- W2947584582 abstract "Biochemical, immunological, and structural characterizations of the structural proteins of simian immunodeficiency virus (SIV) were performed. Various electrophoretic and chromatographic analyses of native and metabolically radiolabeled virus preparations coupled with immunological procedures such as Western blotting and radioimmunoprecipitation were utilized to identify SIV/DeltaB670-encoded polypeptides. Two-dimensional tryptic peptide mapping of each putative viral protein was employed to confirm that each of these components were unique and not cleavage products of precursor polyproteins. Site-directed serological studies using synthetic peptides analogous to regions of the SU and TM protein predicted to have high antigenic potential were performed. These studies resulted in the identification of several group-specific and type-specific antigenic determinants of SIV envelope proteins and demonstrated serological similarities between SIV and several other lentiviruses. The lentivirus lytic peptide (LLP), a segment within the carboxyl terminus of the TM protein which has high amphipathic potential and unusually high positive charge density, was identified during secondary structural modeling studies. Since the LLP appeared to share structural properties with natural cytolytic peptides such as magainins, cecropins, melittin, etc., synthetic peptides homologous to the LLP of HIV-1 and SIV were employed in standard assays designed to measure the ability of cytolytic peptides to inhibit prokaryotic or eukaryotic cells, and were found to share functional characteristics with natural cytolytic peptides. Results from membrane permeability studies and $sp{51}$Cr-release assays suggested that the LLPs inhibit cells via membrane perturbation. The LLPs also share structural characteristics with calmodulin (CaM)-binding proteins. CaM is the primary sensor of Ca$sp{2+}$ within eukaryotic cells. Since Ca$sp{2+}$ is the primary signal for activation of many enzyme systems, CaM plays an essential role in the activation of many cellular enzymatic processes. Using gel mobility shift assays and standard phosphodiesterase competition assays, we have shown that LLPs bind CaM with high affinity and can inhibit CaM-mediated activation of CaM-dependent enzymes. LLP-mediated interference with enzyme activation and/or alteration of critical plasma membrane properties may explain many of the unusual manifestations of HIV-1-mediated disease and may represent a novel mechanism of pathogenesis." @default.
- W2947584582 created "2019-06-07" @default.
- W2947584582 creator A5039674482 @default.
- W2947584582 date "2022-06-14" @default.
- W2947584582 modified "2023-10-07" @default.
- W2947584582 title "Structural, Immunological, and Functional Characterization of Simian Immunodeficiency Virus Proteins." @default.
- W2947584582 cites W131059411 @default.
- W2947584582 cites W137571688 @default.
- W2947584582 cites W1481024063 @default.
- W2947584582 cites W1482593432 @default.
- W2947584582 cites W149313881 @default.
- W2947584582 cites W1499340745 @default.
- W2947584582 cites W1501032637 @default.
- W2947584582 cites W1505517942 @default.
- W2947584582 cites W1510077137 @default.
- W2947584582 cites W1516518493 @default.
- W2947584582 cites W1517691515 @default.
- W2947584582 cites W1517732553 @default.
- W2947584582 cites W1521150511 @default.
- W2947584582 cites W1522259447 @default.
- W2947584582 cites W1526839960 @default.
- W2947584582 cites W1530422829 @default.
- W2947584582 cites W1531831170 @default.
- W2947584582 cites W1538154696 @default.
- W2947584582 cites W1539537611 @default.
- W2947584582 cites W1550645214 @default.
- W2947584582 cites W156522641 @default.
- W2947584582 cites W1568099988 @default.
- W2947584582 cites W1576742764 @default.
- W2947584582 cites W1577222354 @default.
- W2947584582 cites W1578509365 @default.
- W2947584582 cites W1588024596 @default.
- W2947584582 cites W1595736506 @default.
- W2947584582 cites W159935233 @default.
- W2947584582 cites W1602075566 @default.
- W2947584582 cites W1604914713 @default.
- W2947584582 cites W1609333127 @default.
- W2947584582 cites W1634735131 @default.
- W2947584582 cites W1636940011 @default.
- W2947584582 cites W1642163610 @default.
- W2947584582 cites W1649792810 @default.
- W2947584582 cites W1654340299 @default.
- W2947584582 cites W1757031992 @default.
- W2947584582 cites W1762479588 @default.
- W2947584582 cites W1827178381 @default.
- W2947584582 cites W1863034018 @default.
- W2947584582 cites W1899100861 @default.
- W2947584582 cites W1899816681 @default.
- W2947584582 cites W1927697103 @default.
- W2947584582 cites W1930896708 @default.
- W2947584582 cites W1943889027 @default.
- W2947584582 cites W1950530684 @default.
- W2947584582 cites W1965920570 @default.
- W2947584582 cites W1968628374 @default.
- W2947584582 cites W1968873679 @default.
- W2947584582 cites W1969583827 @default.
- W2947584582 cites W1970121767 @default.
- W2947584582 cites W1971951822 @default.
- W2947584582 cites W1972256790 @default.
- W2947584582 cites W1974737906 @default.
- W2947584582 cites W1974819477 @default.
- W2947584582 cites W1975304761 @default.
- W2947584582 cites W1976745468 @default.
- W2947584582 cites W1976970819 @default.
- W2947584582 cites W1977282539 @default.
- W2947584582 cites W1977790220 @default.
- W2947584582 cites W1979050229 @default.
- W2947584582 cites W1979186107 @default.
- W2947584582 cites W1981316974 @default.
- W2947584582 cites W1981864966 @default.
- W2947584582 cites W1981886013 @default.
- W2947584582 cites W1981906666 @default.
- W2947584582 cites W1982646138 @default.
- W2947584582 cites W1983561321 @default.
- W2947584582 cites W1984547849 @default.
- W2947584582 cites W1984754431 @default.
- W2947584582 cites W1984944166 @default.
- W2947584582 cites W1985845861 @default.
- W2947584582 cites W1986117045 @default.
- W2947584582 cites W1986553254 @default.
- W2947584582 cites W1988636987 @default.
- W2947584582 cites W1988867706 @default.
- W2947584582 cites W1988873112 @default.
- W2947584582 cites W1989326908 @default.
- W2947584582 cites W1989426186 @default.
- W2947584582 cites W1990065499 @default.
- W2947584582 cites W1991670386 @default.
- W2947584582 cites W1994825180 @default.
- W2947584582 cites W1995407171 @default.
- W2947584582 cites W1996216405 @default.
- W2947584582 cites W1997271169 @default.
- W2947584582 cites W1997666186 @default.
- W2947584582 cites W1997937309 @default.
- W2947584582 cites W1997949064 @default.
- W2947584582 cites W1999711265 @default.
- W2947584582 cites W2000231527 @default.
- W2947584582 cites W2002292720 @default.
- W2947584582 cites W2003403192 @default.
- W2947584582 cites W2005149743 @default.
- W2947584582 cites W2005347082 @default.