Matches in SemOpenAlex for { <https://semopenalex.org/work/W2947640552> ?p ?o ?g. }
- W2947640552 abstract "Flavonol synthase (FLS) is the key enzyme responsible for the biosynthesis of flavonols, the most abundant flavonoids, which have diverse pharmaceutical effects. Flavonol synthase has been previously found in other species, but not yet in Ornithogalum caudatum.The transcriptome-wide mining and functional characterisation of a flavonol synthase gene family from O. caudatum were reported. Specifically, a small FLS gene family harbouring two members, OcFLS1 and OcFLS2, was isolated from O. caudatum based on transcriptome-wide mining. Phylogenetic analysis suggested that the two proteins showed the closest relationship with FLS proteins. In vitro enzymatic assays indicated OcFLS1 and OcFLS2 were flavonol synthases, catalysing the conversion of dihydroflavonols to flavonols in an iron-dependent fashion. In addition, the two proteins were found to display flavanone 3β-hydroxylase (F3H) activity, hydroxylating flavanones to form dihydroflavonols. Unlike single F3H enzymes, the F3H activity of OcFLS1 and OcFLS2 did not absolutely require iron. However, the presence of sufficient Fe2+ was demonstrated to be conducive to successive catalysis of flavanones to flavonols. The qRT-PCR analysis demonstrated that both genes were expressed in the leaves, bulbs, and flowers, with particularly high expression in the leaves. Moreover, their expression was regulated by developmental and environmental conditions.OcFLS1 and OcFLS2 from O. caudatum were demonstrated to be flavonol synthases with iron-independent flavanone 3-hydroxylase activity." @default.
- W2947640552 created "2019-06-07" @default.
- W2947640552 creator A5067580959 @default.
- W2947640552 creator A5074076936 @default.
- W2947640552 creator A5079316352 @default.
- W2947640552 date "2019-05-14" @default.
- W2947640552 modified "2023-10-18" @default.
- W2947640552 title "Characterization of two flavonol synthases with iron-independent flavanone 3-hydroxylase activity from Ornithogalum caudatum Jacq" @default.
- W2947640552 cites W1948729199 @default.
- W2947640552 cites W1964957783 @default.
- W2947640552 cites W1970468920 @default.
- W2947640552 cites W1975354698 @default.
- W2947640552 cites W1982885679 @default.
- W2947640552 cites W1982925032 @default.
- W2947640552 cites W1986559615 @default.
- W2947640552 cites W1997638141 @default.
- W2947640552 cites W2020390839 @default.
- W2947640552 cites W2021066602 @default.
- W2947640552 cites W2034399234 @default.
- W2947640552 cites W2036987345 @default.
- W2947640552 cites W2038660888 @default.
- W2947640552 cites W2042402699 @default.
- W2947640552 cites W2047382548 @default.
- W2947640552 cites W2062405876 @default.
- W2947640552 cites W2072105294 @default.
- W2947640552 cites W2073076887 @default.
- W2947640552 cites W2073479752 @default.
- W2947640552 cites W2088383365 @default.
- W2947640552 cites W2089820895 @default.
- W2947640552 cites W2090362399 @default.
- W2947640552 cites W2090914471 @default.
- W2947640552 cites W2093951078 @default.
- W2947640552 cites W2122967180 @default.
- W2947640552 cites W2125950656 @default.
- W2947640552 cites W2142498401 @default.
- W2947640552 cites W2238990854 @default.
- W2947640552 cites W2252970784 @default.
- W2947640552 cites W2286621616 @default.
- W2947640552 cites W2312980023 @default.
- W2947640552 cites W2315948853 @default.
- W2947640552 cites W2327246068 @default.
- W2947640552 cites W2327787546 @default.
- W2947640552 cites W2466525550 @default.
- W2947640552 cites W2516246676 @default.
- W2947640552 cites W2528940130 @default.
- W2947640552 cites W2548184016 @default.
- W2947640552 cites W2555021291 @default.
- W2947640552 cites W2560649695 @default.
- W2947640552 cites W2580454126 @default.
- W2947640552 cites W2625702416 @default.
- W2947640552 cites W2725754349 @default.
- W2947640552 cites W2758940571 @default.
- W2947640552 cites W2789790253 @default.
- W2947640552 cites W2797317692 @default.
- W2947640552 cites W2800866502 @default.
- W2947640552 cites W2803522209 @default.
- W2947640552 cites W2809863290 @default.
- W2947640552 cites W2949936212 @default.
- W2947640552 cites W62614531 @default.
- W2947640552 doi "https://doi.org/10.1186/s12870-019-1787-x" @default.
- W2947640552 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6515686" @default.
- W2947640552 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/31088366" @default.
- W2947640552 hasPublicationYear "2019" @default.
- W2947640552 type Work @default.
- W2947640552 sameAs 2947640552 @default.
- W2947640552 citedByCount "12" @default.
- W2947640552 countsByYear W29476405522020 @default.
- W2947640552 countsByYear W29476405522021 @default.
- W2947640552 countsByYear W29476405522022 @default.
- W2947640552 countsByYear W29476405522023 @default.
- W2947640552 crossrefType "journal-article" @default.
- W2947640552 hasAuthorship W2947640552A5067580959 @default.
- W2947640552 hasAuthorship W2947640552A5074076936 @default.
- W2947640552 hasAuthorship W2947640552A5079316352 @default.
- W2947640552 hasBestOaLocation W29476405521 @default.
- W2947640552 hasConcept C104317684 @default.
- W2947640552 hasConcept C150194340 @default.
- W2947640552 hasConcept C162317418 @default.
- W2947640552 hasConcept C181199279 @default.
- W2947640552 hasConcept C193252679 @default.
- W2947640552 hasConcept C2777481717 @default.
- W2947640552 hasConcept C2778004101 @default.
- W2947640552 hasConcept C2779054382 @default.
- W2947640552 hasConcept C2779106036 @default.
- W2947640552 hasConcept C55493867 @default.
- W2947640552 hasConcept C59822182 @default.
- W2947640552 hasConcept C86803240 @default.
- W2947640552 hasConceptScore W2947640552C104317684 @default.
- W2947640552 hasConceptScore W2947640552C150194340 @default.
- W2947640552 hasConceptScore W2947640552C162317418 @default.
- W2947640552 hasConceptScore W2947640552C181199279 @default.
- W2947640552 hasConceptScore W2947640552C193252679 @default.
- W2947640552 hasConceptScore W2947640552C2777481717 @default.
- W2947640552 hasConceptScore W2947640552C2778004101 @default.
- W2947640552 hasConceptScore W2947640552C2779054382 @default.
- W2947640552 hasConceptScore W2947640552C2779106036 @default.
- W2947640552 hasConceptScore W2947640552C55493867 @default.
- W2947640552 hasConceptScore W2947640552C59822182 @default.
- W2947640552 hasConceptScore W2947640552C86803240 @default.
- W2947640552 hasIssue "1" @default.