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- W2947693589 abstract "In the years following its discovery and characterization, the CRISPR-Cas system has been modified and converted into a multitude of applications for eukaryotes and bacteria, such as genome editing and gene regulation. Since no such method has been available for archaea, we developed a tool for gene repression in the haloarchaeon Haloferax volcanii by repurposing its endogenous type I-B CRISPR-Cas system. Here, we present the two possible approaches for gene repression as well as our workflow to achieve and assess gene knockdown, offer recommendations on protospacer selection and give some examples of genes we have successfully silenced." @default.
- W2947693589 created "2019-06-07" @default.
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- W2947693589 date "2020-02-01" @default.
- W2947693589 modified "2023-10-01" @default.
- W2947693589 title "CRISPRi as an efficient tool for gene repression in archaea" @default.
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- W2947693589 doi "https://doi.org/10.1016/j.ymeth.2019.05.023" @default.
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