FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2947723046> ?p ?o ?g. }

• W2947723046 endingPage "103565" @default.
• W2947723046 startingPage "103565" @default.
• W2947723046 abstract "Haemophilus parasuis (H. parasuis) is rather difficult to manipulate genetically due to the diversity of restriction-modification systems and other mechanisms harbored by various isolates. This prevents exogenous plasmids from replicating in this species and hinders research efforts focused on transcriptional regulators in this bacterium. In this study, we generated a convenient promoter reporter system based on gene knock-in method using natural transformation in H. parasuis. Gene knock-in has proven useful as a powerful tool facilitating identification and studying the transcription activities of regulators under a variety of conditions that favor gene transcription or expression from an incorporated promoter. The vectors, pDK-K and pDK-G, carrying promoterless reporter lacZ gene and two homologous sequences flanking a knock-in site, may have some advantages over the extensively used plasmid-bearing reporter system in other bacteria in stability and ease of genetic manipulation in H. parasuis. The knock-in site was positioned at a site occupied by flanking genes that were both hypothetical and had the same transcription orientation, thus the expression of the reversely cloned promoter-lacZ fusion wouldn't be affected by the upstream promoter on the chromosome. The expression activity of lacZ gene under the transcriptional activation of a 300 bp promoter-proximal segment of cyaA, crp or comA genes in H. parasuis was separately validated using X-gal and o-nitrophenyl-β-d-galactoside(ONPG) as substrates. The derivatives harboring promoter-lacZ fusion segments showed significantly higher β-galactosidase activity levels than the promoterlessones both in TSB++ broth and on TSA++ plate as screened either by X-gal method or the standard Miller method. We also used pDK vector to further certify that the cyaA promoter is inducible and whose transcriptional levels were in correlation with the growth kinetics of the bacteria in TSB++. With this system, gene knock-in method based on natural transformation in H. parasuis proved to be useful in identifying transcriptional regulation of a certain promoter." @default.
• W2947723046 created "2019-06-07" @default.
• W2947723046 creator A5012266709 @default.
• W2947723046 creator A5013725855 @default.
• W2947723046 creator A5013864491 @default.
• W2947723046 creator A5015158206 @default.
• W2947723046 creator A5015568621 @default.
• W2947723046 creator A5020392820 @default.
• W2947723046 creator A5024897452 @default.
• W2947723046 creator A5025305947 @default.
• W2947723046 creator A5026834273 @default.
• W2947723046 creator A5029226448 @default.
• W2947723046 creator A5032600404 @default.
• W2947723046 creator A5042718279 @default.
• W2947723046 creator A5044586464 @default.
• W2947723046 creator A5085619990 @default.
• W2947723046 date "2019-09-01" @default.
• W2947723046 modified "2023-09-23" @default.
• W2947723046 title "Construction of targeted and integrative promoter-reporter plasmids pDK-K and pDK-G to measure gene expression activity in Haemophilus parasuis" @default.
• W2947723046 cites W1581397724 @default.
• W2947723046 cites W1975182786 @default.
• W2947723046 cites W2019033752 @default.
• W2947723046 cites W2024262177 @default.
• W2947723046 cites W2033220306 @default.
• W2947723046 cites W2039901145 @default.
• W2947723046 cites W2046167310 @default.
• W2947723046 cites W2048456982 @default.
• W2947723046 cites W2053196131 @default.
• W2947723046 cites W2062848147 @default.
• W2947723046 cites W2090040402 @default.
• W2947723046 cites W2100009016 @default.
• W2947723046 cites W2116137883 @default.
• W2947723046 cites W2120410982 @default.
• W2947723046 cites W2127901301 @default.
• W2947723046 cites W2128031707 @default.
• W2947723046 cites W2136961240 @default.
• W2947723046 cites W2141149764 @default.
• W2947723046 cites W2153076508 @default.
• W2947723046 cites W2154724920 @default.
• W2947723046 cites W2182863032 @default.
• W2947723046 cites W2270374520 @default.
• W2947723046 cites W2403106021 @default.
• W2947723046 cites W2500261702 @default.
• W2947723046 cites W2511682228 @default.
• W2947723046 cites W2530293438 @default.
• W2947723046 cites W2542410221 @default.
• W2947723046 cites W2599327651 @default.
• W2947723046 cites W2604956202 @default.
• W2947723046 cites W2611308544 @default.
• W2947723046 cites W2765403431 @default.
• W2947723046 cites W2778788489 @default.
• W2947723046 cites W2789783177 @default.
• W2947723046 cites W2807480035 @default.
• W2947723046 cites W2829481776 @default.
• W2947723046 doi "https://doi.org/10.1016/j.micpath.2019.103565" @default.
• W2947723046 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/31158493" @default.
• W2947723046 hasPublicationYear "2019" @default.
• W2947723046 type Work @default.
• W2947723046 sameAs 2947723046 @default.
• W2947723046 citedByCount "5" @default.
• W2947723046 countsByYear W29477230462019 @default.
• W2947723046 countsByYear W29477230462021 @default.
• W2947723046 countsByYear W29477230462022 @default.
• W2947723046 countsByYear W29477230462023 @default.
• W2947723046 crossrefType "journal-article" @default.
• W2947723046 hasAuthorship W2947723046A5012266709 @default.
• W2947723046 hasAuthorship W2947723046A5013725855 @default.
• W2947723046 hasAuthorship W2947723046A5013864491 @default.
• W2947723046 hasAuthorship W2947723046A5015158206 @default.
• W2947723046 hasAuthorship W2947723046A5015568621 @default.
• W2947723046 hasAuthorship W2947723046A5020392820 @default.
• W2947723046 hasAuthorship W2947723046A5024897452 @default.
• W2947723046 hasAuthorship W2947723046A5025305947 @default.
• W2947723046 hasAuthorship W2947723046A5026834273 @default.
• W2947723046 hasAuthorship W2947723046A5029226448 @default.
• W2947723046 hasAuthorship W2947723046A5032600404 @default.
• W2947723046 hasAuthorship W2947723046A5042718279 @default.
• W2947723046 hasAuthorship W2947723046A5044586464 @default.
• W2947723046 hasAuthorship W2947723046A5085619990 @default.
• W2947723046 hasConcept C101762097 @default.
• W2947723046 hasConcept C104317684 @default.
• W2947723046 hasConcept C138885662 @default.
• W2947723046 hasConcept C150194340 @default.
• W2947723046 hasConcept C153911025 @default.
• W2947723046 hasConcept C161733203 @default.
• W2947723046 hasConcept C165864922 @default.
• W2947723046 hasConcept C170344550 @default.
• W2947723046 hasConcept C179926584 @default.
• W2947723046 hasConcept C21592294 @default.
• W2947723046 hasConcept C22744801 @default.
• W2947723046 hasConcept C41895202 @default.
• W2947723046 hasConcept C54355233 @default.
• W2947723046 hasConcept C86803240 @default.
• W2947723046 hasConceptScore W2947723046C101762097 @default.
• W2947723046 hasConceptScore W2947723046C104317684 @default.
• W2947723046 hasConceptScore W2947723046C138885662 @default.
• W2947723046 hasConceptScore W2947723046C150194340 @default.
• W2947723046 hasConceptScore W2947723046C153911025 @default.

• next