FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2947730224> ?p ?o ?g. }

• W2947730224 abstract "Previous studies have reported poor proliferation and bioactivity of human anterior cruciate ligament fibroblasts (hACLFs) after injury. As hACLFs are one of the most significant and indispensable source of seed cells in constructing tissue-engineered ligament, enhancing hACLF proliferation would offer favorable cellular-biological ability and induce the extracellular matrix secretion of hACLFs after loading on multiple types of scaffolds. Enhancing the bioactivity of hACLFs would improve tissue repair and functional recovery after tissue-engineered ligament transplantation. This study compared cells prepared by collagenase digestion and the in situ culture of tissue pieces and investigated the effect of basic fibroblast growth factor (bFGF) on hACLFs.Six adult patients participated in this study. Of these patients, tissues from three were compared after culture establishment through collagenase digestion or in situ tissue isolation. hACLF phenotypic characteristics were assessed, and the effect of bFGF on hACLF cultures was observed. hACLFs cultured with and without bFGF served as the experimental and control groups, respectively. Cell Counting Kit-8 was used to detect proliferation. The expression of ligament-related genes and proteins was evaluated by immunofluorescence staining, real-time polymerase chain reaction (PCR) assays, and Western blot assays.The morphology of hACLFs isolated using the two methods differed after the 2nd passage. The proliferation of cells obtained by in situ culture was higher than that of cells obtained by collagenase digestion. hACLFs cultured with bFGF after the 3rd passage exhibited a higher proliferation rate than the controls. Immunofluorescence staining, real-time PCR, and Western blot analysis showed a significant increase in ligament-related gene and protein expression in the hACLFs cultured with bFGF.The in situ isolation of tissue pieces enhanced hACLF proliferation in vitro, and the hACLFs exhibited phenotypic characteristics of fibroblasts. hACLFs cultured with bFGF exhibited increased hACLF bioactivity." @default.
• W2947730224 created "2019-06-07" @default.
• W2947730224 creator A5000515886 @default.
• W2947730224 creator A5003524871 @default.
• W2947730224 creator A5006385041 @default.
• W2947730224 creator A5007000778 @default.
• W2947730224 creator A5008659449 @default.
• W2947730224 creator A5008863671 @default.
• W2947730224 creator A5015823958 @default.
• W2947730224 creator A5029119899 @default.
• W2947730224 creator A5073440135 @default.
• W2947730224 creator A5076825933 @default.
• W2947730224 creator A5080654961 @default.
• W2947730224 date "2019-05-01" @default.
• W2947730224 modified "2023-10-18" @default.
• W2947730224 title "Enhancement of in vitro proliferation and bioactivity of human anterior cruciate ligament fibroblasts using an in situ tissue isolation method and basic fibroblast growth factor culture conditions" @default.
• W2947730224 cites W1255984457 @default.
• W2947730224 cites W1920934924 @default.
• W2947730224 cites W1973350053 @default.
• W2947730224 cites W1984228459 @default.
• W2947730224 cites W1984429829 @default.
• W2947730224 cites W1997253527 @default.
• W2947730224 cites W2006235350 @default.
• W2947730224 cites W2011115952 @default.
• W2947730224 cites W2018061385 @default.
• W2947730224 cites W2024021910 @default.
• W2947730224 cites W2048762270 @default.
• W2947730224 cites W2071352906 @default.
• W2947730224 cites W2091847272 @default.
• W2947730224 cites W2104018680 @default.
• W2947730224 cites W2149238770 @default.
• W2947730224 cites W2152543461 @default.
• W2947730224 cites W2236886743 @default.
• W2947730224 cites W2276783448 @default.
• W2947730224 cites W2299081204 @default.
• W2947730224 cites W2343701512 @default.
• W2947730224 cites W2343967025 @default.
• W2947730224 cites W2378453630 @default.
• W2947730224 cites W2381411745 @default.
• W2947730224 cites W2406623206 @default.
• W2947730224 cites W2413802090 @default.
• W2947730224 cites W2413918001 @default.
• W2947730224 cites W2460812498 @default.
• W2947730224 cites W2465207001 @default.
• W2947730224 cites W2473346314 @default.
• W2947730224 cites W2505679689 @default.
• W2947730224 cites W2509970235 @default.
• W2947730224 cites W2511855551 @default.
• W2947730224 cites W2513176365 @default.
• W2947730224 cites W2518426247 @default.
• W2947730224 cites W2526364304 @default.
• W2947730224 cites W2527915711 @default.
• W2947730224 cites W2528296897 @default.
• W2947730224 cites W2529473727 @default.
• W2947730224 cites W2531917425 @default.
• W2947730224 cites W2615386951 @default.
• W2947730224 cites W2726257799 @default.
• W2947730224 cites W2755979383 @default.
• W2947730224 cites W2902287556 @default.
• W2947730224 cites W4236336358 @default.
• W2947730224 cites W985667476 @default.
• W2947730224 doi "https://doi.org/10.1097/md.0000000000015907" @default.
• W2947730224 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6708876" @default.
• W2947730224 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/31145353" @default.
• W2947730224 hasPublicationYear "2019" @default.
• W2947730224 type Work @default.
• W2947730224 sameAs 2947730224 @default.
• W2947730224 citedByCount "0" @default.
• W2947730224 crossrefType "journal-article" @default.
• W2947730224 hasAuthorship W2947730224A5000515886 @default.
• W2947730224 hasAuthorship W2947730224A5003524871 @default.
• W2947730224 hasAuthorship W2947730224A5006385041 @default.
• W2947730224 hasAuthorship W2947730224A5007000778 @default.
• W2947730224 hasAuthorship W2947730224A5008659449 @default.
• W2947730224 hasAuthorship W2947730224A5008863671 @default.
• W2947730224 hasAuthorship W2947730224A5015823958 @default.
• W2947730224 hasAuthorship W2947730224A5029119899 @default.
• W2947730224 hasAuthorship W2947730224A5073440135 @default.
• W2947730224 hasAuthorship W2947730224A5076825933 @default.
• W2947730224 hasAuthorship W2947730224A5080654961 @default.
• W2947730224 hasBestOaLocation W29477302241 @default.
• W2947730224 hasConcept C104317684 @default.
• W2947730224 hasConcept C105702510 @default.
• W2947730224 hasConcept C136229726 @default.
• W2947730224 hasConcept C153911025 @default.
• W2947730224 hasConcept C170493617 @default.
• W2947730224 hasConcept C181199279 @default.
• W2947730224 hasConcept C189165786 @default.
• W2947730224 hasConcept C202751555 @default.
• W2947730224 hasConcept C2775960820 @default.
• W2947730224 hasConcept C2776415932 @default.
• W2947730224 hasConcept C2777587049 @default.
• W2947730224 hasConcept C2778394429 @default.
• W2947730224 hasConcept C2778434673 @default.
• W2947730224 hasConcept C2780381497 @default.
• W2947730224 hasConcept C49892992 @default.
• W2947730224 hasConcept C54355233 @default.
• W2947730224 hasConcept C55493867 @default.
• W2947730224 hasConcept C62112901 @default.
• W2947730224 hasConcept C71924100 @default.

• next