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• W2947758507 abstract "Hypoxia is a common feature in solid tumors. Clinical samples show a positive correlation between the expression of the hypoxia-inducible factor HIF-1α and estrogen receptor alpha (ERα) and a negative correlation between HIF-1α and hormone sensitivity. Results from monolayer cultures are in contention with clinical observations, showing that ER (+) cell lines no longer express ERα under hypoxic conditions (1% O2). Here, we compared the impact of hypoxia on the ERα signaling pathway for T47D cells in a 2D and 3D culture format. In the 2D format, the cells were cultured as monolayers. In the 3D format, paper-based scaffolds supported cells suspended in a collagen matrix. Using ELISA, Western blot, and immunofluorescence measurements, we show that hypoxia differentially regulates ERα protein levels in a culture environment-dependent manner. In the 2D format, the protein levels are significantly decreased in hypoxia. In the 3D format, the protein levels are maintained in hypoxia. Hypoxia reduced ERα transcriptional activation in both culture formats. These results highlight the importance of considering tissue dimensionality for in vitro studies. They also show that ERα protein levels in hypoxia are not an accurate indicator of ERα transcriptional activity, and confirm that a positive stain for ERα in a clinical sample may not necessarily indicate hormone sensitivity." @default.
• W2947758507 created "2019-06-07" @default.
• W2947758507 creator A5004973093 @default.
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• W2947758507 creator A5026674463 @default.
• W2947758507 creator A5040766930 @default.
• W2947758507 creator A5088456312 @default.
• W2947758507 date "2019-08-01" @default.
• W2947758507 modified "2023-09-25" @default.
• W2947758507 title "Hypoxia differentially regulates estrogen receptor alpha in 2D and 3D culture formats" @default.
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• W2947758507 cites W1982418282 @default.
• W2947758507 cites W1982620171 @default.
• W2947758507 cites W1983486852 @default.
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• W2947758507 cites W1991880013 @default.
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• W2947758507 cites W2013735638 @default.
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• W2947758507 doi "https://doi.org/10.1016/j.abb.2019.05.025" @default.
• W2947758507 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6688900" @default.
• W2947758507 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/31163125" @default.
• W2947758507 hasPublicationYear "2019" @default.
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