FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2947804917> ?p ?o ?g. }

• W2947804917 endingPage "2525" @default.
• W2947804917 startingPage "2514" @default.
• W2947804917 abstract "Histone post-translational modifications (PTMs) have been intensively investigated due to their essential function in eukaryotic genome regulation. Histone modifications have been effectively studied using modified bottom-up proteomics approaches; however, the methods often do not capture single-molecule combinations of PTMs (proteoforms) that mediate known and expected biochemical mechanisms. Both middle-down mass spectrometry (MS) and top-down MS quantitation of H4 proteoforms present viable access to this important information. Histone H4 middle-down has previously avoided GluC digestion due to complex digestion products and interferences; however, the common AspN digestion cleaves at amino acid 23, disconnecting K31ac from other PTMs. Here, we demonstrate the effective use of GluC-based middle-down quantitation and compare it to top-down-based quantitation of proteoforms. Despite potential interferences in the m/z space, the proteoforms arising from all three GluC products (E52, E53, and E63) and intact H4 are chromatographically resolved and successfully analyzed in a single LC–MS analysis. Quantitative results and associated analytical metrics are compared between the different analytes of a single sample digested to different extents to reveal general concordance as well as the relative biases and complementarity of each approach. There is moderate proteoform discordance between digestion products (e.g., E52 and E53); however, each digestion product exhibits high concordance, regardless of digestion time. Under the conditions used, the GluC products are better chromatographically resolved yet show greater variance than the top-down quantitation that are more extensively sampled for MS2. GluC-based middle-down of H4 is thus viable. Both top-down and middle-down approaches have comparable quantitation capacity and are complementary." @default.
• W2947804917 created "2019-06-07" @default.
• W2947804917 creator A5009228621 @default.
• W2947804917 creator A5014871443 @default.
• W2947804917 creator A5076566463 @default.
• W2947804917 date "2019-05-30" @default.
• W2947804917 modified "2023-10-18" @default.
• W2947804917 title "One-Pot Quantitative Top- and Middle-Down Analysis of GluC-Digested Histone H4" @default.
• W2947804917 cites W1567904427 @default.
• W2947804917 cites W1582516724 @default.
• W2947804917 cites W1905086641 @default.
• W2947804917 cites W1968931043 @default.
• W2947804917 cites W1993428821 @default.
• W2947804917 cites W2016025530 @default.
• W2947804917 cites W2032091997 @default.
• W2947804917 cites W2040141444 @default.
• W2947804917 cites W2058104615 @default.
• W2947804917 cites W2061805545 @default.
• W2947804917 cites W2063529845 @default.
• W2947804917 cites W2073123664 @default.
• W2947804917 cites W2084699632 @default.
• W2947804917 cites W2089924497 @default.
• W2947804917 cites W2099088617 @default.
• W2947804917 cites W2102273403 @default.
• W2947804917 cites W2116993297 @default.
• W2947804917 cites W2142826475 @default.
• W2947804917 cites W2143103377 @default.
• W2947804917 cites W2144792857 @default.
• W2947804917 cites W2149407896 @default.
• W2947804917 cites W2244029933 @default.
• W2947804917 cites W2406278632 @default.
• W2947804917 cites W2553171784 @default.
• W2947804917 cites W2693253431 @default.
• W2947804917 cites W2729636480 @default.
• W2947804917 cites W2732957153 @default.
• W2947804917 cites W2765270718 @default.
• W2947804917 cites W2800060138 @default.
• W2947804917 cites W2801552223 @default.
• W2947804917 cites W2805607853 @default.
• W2947804917 cites W2811283524 @default.
• W2947804917 cites W2899760200 @default.
• W2947804917 doi "https://doi.org/10.1007/s13361-019-02219-1" @default.
• W2947804917 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/31147891" @default.
• W2947804917 hasPublicationYear "2019" @default.
• W2947804917 type Work @default.
• W2947804917 sameAs 2947804917 @default.
• W2947804917 citedByCount "11" @default.
• W2947804917 countsByYear W29478049172019 @default.
• W2947804917 countsByYear W29478049172020 @default.
• W2947804917 countsByYear W29478049172021 @default.
• W2947804917 countsByYear W29478049172022 @default.
• W2947804917 countsByYear W29478049172023 @default.
• W2947804917 crossrefType "journal-article" @default.
• W2947804917 hasAuthorship W2947804917A5009228621 @default.
• W2947804917 hasAuthorship W2947804917A5014871443 @default.
• W2947804917 hasAuthorship W2947804917A5076566463 @default.
• W2947804917 hasConcept C10390740 @default.
• W2947804917 hasConcept C104317684 @default.
• W2947804917 hasConcept C13762613 @default.
• W2947804917 hasConcept C162356407 @default.
• W2947804917 hasConcept C185592680 @default.
• W2947804917 hasConcept C2779085176 @default.
• W2947804917 hasConcept C2781357212 @default.
• W2947804917 hasConcept C31827203 @default.
• W2947804917 hasConcept C43617362 @default.
• W2947804917 hasConcept C46111723 @default.
• W2947804917 hasConcept C55493867 @default.
• W2947804917 hasConcept C64927066 @default.
• W2947804917 hasConcept C70721500 @default.
• W2947804917 hasConcept C71043704 @default.
• W2947804917 hasConcept C86803240 @default.
• W2947804917 hasConceptScore W2947804917C10390740 @default.
• W2947804917 hasConceptScore W2947804917C104317684 @default.
• W2947804917 hasConceptScore W2947804917C13762613 @default.
• W2947804917 hasConceptScore W2947804917C162356407 @default.
• W2947804917 hasConceptScore W2947804917C185592680 @default.
• W2947804917 hasConceptScore W2947804917C2779085176 @default.
• W2947804917 hasConceptScore W2947804917C2781357212 @default.
• W2947804917 hasConceptScore W2947804917C31827203 @default.
• W2947804917 hasConceptScore W2947804917C43617362 @default.
• W2947804917 hasConceptScore W2947804917C46111723 @default.
• W2947804917 hasConceptScore W2947804917C55493867 @default.
• W2947804917 hasConceptScore W2947804917C64927066 @default.
• W2947804917 hasConceptScore W2947804917C70721500 @default.
• W2947804917 hasConceptScore W2947804917C71043704 @default.
• W2947804917 hasConceptScore W2947804917C86803240 @default.
• W2947804917 hasIssue "12" @default.
• W2947804917 hasLocation W29478049171 @default.
• W2947804917 hasLocation W29478049172 @default.
• W2947804917 hasOpenAccess W2947804917 @default.
• W2947804917 hasPrimaryLocation W29478049171 @default.
• W2947804917 hasRelatedWork W1944150046 @default.
• W2947804917 hasRelatedWork W1975062759 @default.
• W2947804917 hasRelatedWork W2041872166 @default.
• W2947804917 hasRelatedWork W2061679850 @default.
• W2947804917 hasRelatedWork W2071548110 @default.
• W2947804917 hasRelatedWork W2082761856 @default.
• W2947804917 hasRelatedWork W2114677424 @default.

• next