Matches in SemOpenAlex for { <https://semopenalex.org/work/W2947867835> ?p ?o ?g. }
- W2947867835 endingPage "2033" @default.
- W2947867835 startingPage "2033" @default.
- W2947867835 abstract "Human milk oligosaccharides (HMOs) signify a unique group of oligosaccharides in breast milk, which is of major importance for infant health and development. The functional benefits of HMOs create an enormous impetus for biosynthetic production of HMOs for use as additives in infant formula and other products. HMO molecules can be synthesized chemically, via fermentation, and by enzymatic synthesis. This treatise discusses these different techniques, with particular focus on harnessing enzymes for controlled enzymatic synthesis of HMO molecules. In order to foster precise and high-yield enzymatic synthesis, several novel protein engineering approaches have been reported, mainly concerning changing glycoside hydrolases to catalyze relevant transglycosylations. The protein engineering strategies for these enzymes range from rationally modifying specific catalytic residues, over targeted subsite -1 mutations, to unique and novel transplantations of designed peptide sequences near the active site, so-called loop engineering. These strategies have proven useful to foster enhanced transglycosylation to promote different types of HMO synthesis reactions. The rationale of subsite -1 modification, acceptor binding site matching, and loop engineering, including changes that may alter the spatial arrangement of water in the enzyme active site region, may prove useful for novel enzyme-catalyzed carbohydrate design in general." @default.
- W2947867835 created "2019-06-07" @default.
- W2947867835 creator A5015687354 @default.
- W2947867835 creator A5071750358 @default.
- W2947867835 creator A5075678104 @default.
- W2947867835 creator A5085720748 @default.
- W2947867835 date "2019-05-28" @default.
- W2947867835 modified "2023-10-17" @default.
- W2947867835 title "Synthesis of Human Milk Oligosaccharides: Protein Engineering Strategies for Improved Enzymatic Transglycosylation" @default.
- W2947867835 cites W107348671 @default.
- W2947867835 cites W1492345118 @default.
- W2947867835 cites W1494943313 @default.
- W2947867835 cites W1580799101 @default.
- W2947867835 cites W1905217066 @default.
- W2947867835 cites W1910193362 @default.
- W2947867835 cites W1935712031 @default.
- W2947867835 cites W1935931219 @default.
- W2947867835 cites W1938656015 @default.
- W2947867835 cites W1968827093 @default.
- W2947867835 cites W1969718038 @default.
- W2947867835 cites W1971197481 @default.
- W2947867835 cites W1973324144 @default.
- W2947867835 cites W1974345820 @default.
- W2947867835 cites W1978483664 @default.
- W2947867835 cites W1979800297 @default.
- W2947867835 cites W1981372363 @default.
- W2947867835 cites W1985290172 @default.
- W2947867835 cites W1986549910 @default.
- W2947867835 cites W1989202961 @default.
- W2947867835 cites W1990199609 @default.
- W2947867835 cites W1993390622 @default.
- W2947867835 cites W1994569012 @default.
- W2947867835 cites W1997252922 @default.
- W2947867835 cites W2007404842 @default.
- W2947867835 cites W2007903486 @default.
- W2947867835 cites W2015361638 @default.
- W2947867835 cites W2015642483 @default.
- W2947867835 cites W2024254387 @default.
- W2947867835 cites W2028352823 @default.
- W2947867835 cites W2030313011 @default.
- W2947867835 cites W2031275072 @default.
- W2947867835 cites W2034297086 @default.
- W2947867835 cites W2034581962 @default.
- W2947867835 cites W2035372223 @default.
- W2947867835 cites W2045545069 @default.
- W2947867835 cites W2048524007 @default.
- W2947867835 cites W2053575361 @default.
- W2947867835 cites W2053700373 @default.
- W2947867835 cites W2055702386 @default.
- W2947867835 cites W2064129151 @default.
- W2947867835 cites W2064178181 @default.
- W2947867835 cites W2064334637 @default.
- W2947867835 cites W2064911516 @default.
- W2947867835 cites W2065395992 @default.
- W2947867835 cites W2070282043 @default.
- W2947867835 cites W2071417907 @default.
- W2947867835 cites W2074948045 @default.
- W2947867835 cites W2075559014 @default.
- W2947867835 cites W2076364921 @default.
- W2947867835 cites W2079626310 @default.
- W2947867835 cites W2082979878 @default.
- W2947867835 cites W2084438319 @default.
- W2947867835 cites W2086024565 @default.
- W2947867835 cites W2089805719 @default.
- W2947867835 cites W2091075510 @default.
- W2947867835 cites W2093995674 @default.
- W2947867835 cites W2094566976 @default.
- W2947867835 cites W2105188644 @default.
- W2947867835 cites W2108031998 @default.
- W2947867835 cites W2108459566 @default.
- W2947867835 cites W2111037012 @default.
- W2947867835 cites W2114026324 @default.
- W2947867835 cites W2114355411 @default.
- W2947867835 cites W2115643526 @default.
- W2947867835 cites W2116601591 @default.
- W2947867835 cites W2122271812 @default.
- W2947867835 cites W2122559203 @default.
- W2947867835 cites W2128956666 @default.
- W2947867835 cites W2132517138 @default.
- W2947867835 cites W2132537788 @default.
- W2947867835 cites W2135541178 @default.
- W2947867835 cites W2140632412 @default.
- W2947867835 cites W2141047359 @default.
- W2947867835 cites W2141589632 @default.
- W2947867835 cites W2147847097 @default.
- W2947867835 cites W2149001055 @default.
- W2947867835 cites W2159921444 @default.
- W2947867835 cites W2171924024 @default.
- W2947867835 cites W2172100516 @default.
- W2947867835 cites W2178616046 @default.
- W2947867835 cites W2187348079 @default.
- W2947867835 cites W2190199302 @default.
- W2947867835 cites W2195422852 @default.
- W2947867835 cites W2226622075 @default.
- W2947867835 cites W2267356042 @default.
- W2947867835 cites W2288347709 @default.
- W2947867835 cites W2293739757 @default.
- W2947867835 cites W2297525844 @default.