Matches in SemOpenAlex for { <https://semopenalex.org/work/W2948381569> ?p ?o ?g. }
Showing items 1 to 80 of
80
with 100 items per page.
- W2948381569 endingPage "P" @default.
- W2948381569 startingPage "2000" @default.
- W2948381569 abstract "White adipose tissue (WAT) expands with angiogenesis. We have demonstrated that adipose-tissue-macrophages (ATMs) highly express platelet-derived growth factor (PDGF)-B in obesity, and exposure of PDGF-B stimulates detachment of pericytes (PCs) from vessels. Since vessel-attached PCs inhibit endothelial cell growth, the detachment restarts angiogenesis. In the present study, we aimed to clarify the significance of ATMs on adipose angiogenesis. Intraperitoneal injection of liposome-encapsulated clodronate (Clod) for 6 weeks achieved depletion of macrophages and decreased expression of Pdgfb mRNA in WAT of HFD-fed mice. Clod-treated mice showed smaller WAT with vessels well covered with PCs compared to control mice. We next investigated underlying mechanism of Pdgfb induction. LPS but not IL-4 stimulated Pdgfb expression in peritoneal macrophages. In RAW 264.7 macrophages, Pdgfb expression induced by high glucose plus LPS stimulation was completely blocked by pretreatment with 2-deoxyglucose (2-DG), a hexokinase inhibitor or heptelidic acid, a GAPDH inhibitor, whereas it was not affected by treatment with 6-aminonicotinamide, an inhibitor of glucose-6-phosphate dehydrogenase acting as the rate-limiting enzyme of the pentose phosphate pathway. In addition, high glucose plus LPS induced phosphorylations of S6 kinase, ERK, and JNK but not p38 MAP kinase. These phosphorylations were significantly attenuated by treatment with 2DG. Importantly, enhanced expression of Pdgfb by high glucose plus LPS was effectively suppressed by inhibitor or knockdown of Erk but not by rapamycin. The expression was also attenuated by inhibition of NFκb pathway." @default.
- W2948381569 created "2019-06-14" @default.
- W2948381569 creator A5003849424 @default.
- W2948381569 creator A5043159805 @default.
- W2948381569 creator A5053712244 @default.
- W2948381569 creator A5056021133 @default.
- W2948381569 creator A5065944977 @default.
- W2948381569 creator A5072998209 @default.
- W2948381569 creator A5085854052 @default.
- W2948381569 creator A5089487449 @default.
- W2948381569 date "2019-06-01" @default.
- W2948381569 modified "2023-09-25" @default.
- W2948381569 title "2000-P: Intracellular Metabolism-Dependent PDGF-B Induction in Inflammatory Macrophages Contributes to Adipose Tissue Expansion with Obesity" @default.
- W2948381569 doi "https://doi.org/10.2337/db19-2000-p" @default.
- W2948381569 hasPublicationYear "2019" @default.
- W2948381569 type Work @default.
- W2948381569 sameAs 2948381569 @default.
- W2948381569 citedByCount "0" @default.
- W2948381569 crossrefType "journal-article" @default.
- W2948381569 hasAuthorship W2948381569A5003849424 @default.
- W2948381569 hasAuthorship W2948381569A5043159805 @default.
- W2948381569 hasAuthorship W2948381569A5053712244 @default.
- W2948381569 hasAuthorship W2948381569A5056021133 @default.
- W2948381569 hasAuthorship W2948381569A5065944977 @default.
- W2948381569 hasAuthorship W2948381569A5072998209 @default.
- W2948381569 hasAuthorship W2948381569A5085854052 @default.
- W2948381569 hasAuthorship W2948381569A5089487449 @default.
- W2948381569 hasConcept C11960822 @default.
- W2948381569 hasConcept C126322002 @default.
- W2948381569 hasConcept C134018914 @default.
- W2948381569 hasConcept C170493617 @default.
- W2948381569 hasConcept C171089720 @default.
- W2948381569 hasConcept C180361614 @default.
- W2948381569 hasConcept C184235292 @default.
- W2948381569 hasConcept C185592680 @default.
- W2948381569 hasConcept C2775960820 @default.
- W2948381569 hasConcept C2780394083 @default.
- W2948381569 hasConcept C2780545995 @default.
- W2948381569 hasConcept C57074206 @default.
- W2948381569 hasConcept C71924100 @default.
- W2948381569 hasConcept C75217442 @default.
- W2948381569 hasConcept C86803240 @default.
- W2948381569 hasConcept C95444343 @default.
- W2948381569 hasConceptScore W2948381569C11960822 @default.
- W2948381569 hasConceptScore W2948381569C126322002 @default.
- W2948381569 hasConceptScore W2948381569C134018914 @default.
- W2948381569 hasConceptScore W2948381569C170493617 @default.
- W2948381569 hasConceptScore W2948381569C171089720 @default.
- W2948381569 hasConceptScore W2948381569C180361614 @default.
- W2948381569 hasConceptScore W2948381569C184235292 @default.
- W2948381569 hasConceptScore W2948381569C185592680 @default.
- W2948381569 hasConceptScore W2948381569C2775960820 @default.
- W2948381569 hasConceptScore W2948381569C2780394083 @default.
- W2948381569 hasConceptScore W2948381569C2780545995 @default.
- W2948381569 hasConceptScore W2948381569C57074206 @default.
- W2948381569 hasConceptScore W2948381569C71924100 @default.
- W2948381569 hasConceptScore W2948381569C75217442 @default.
- W2948381569 hasConceptScore W2948381569C86803240 @default.
- W2948381569 hasConceptScore W2948381569C95444343 @default.
- W2948381569 hasIssue "Supplement 1" @default.
- W2948381569 hasLocation W29483815691 @default.
- W2948381569 hasOpenAccess W2948381569 @default.
- W2948381569 hasPrimaryLocation W29483815691 @default.
- W2948381569 hasRelatedWork W1959188680 @default.
- W2948381569 hasRelatedWork W1984583460 @default.
- W2948381569 hasRelatedWork W2033255624 @default.
- W2948381569 hasRelatedWork W2089385669 @default.
- W2948381569 hasRelatedWork W2102224828 @default.
- W2948381569 hasRelatedWork W2315114303 @default.
- W2948381569 hasRelatedWork W2320824175 @default.
- W2948381569 hasRelatedWork W2359268020 @default.
- W2948381569 hasRelatedWork W3152149782 @default.
- W2948381569 hasRelatedWork W4313380913 @default.
- W2948381569 hasVolume "68" @default.
- W2948381569 isParatext "false" @default.
- W2948381569 isRetracted "false" @default.
- W2948381569 magId "2948381569" @default.
- W2948381569 workType "article" @default.