FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2948465206> ?p ?o ?g. }

• W2948465206 endingPage "067003" @default.
• W2948465206 startingPage "067003" @default.
• W2948465206 abstract "Background: The pyrethroid deltamethrin (DM) is broadly used for insect control. Although DM hyperexcites neuronal networks by delaying inactivation of axonal voltage-dependent Na+ channels, this mechanism is unlikely to mediate neurotoxicity at lower exposure levels during critical perinatal periods in mammals. Objectives: We aimed to identify mechanisms by which acute and subchronic DM altered axonal and dendritic growth, patterns of synchronous Ca2+ oscillations (SCOs), and electrical spike activity (ESA) functions critical to neuronal network formation. Methods: Measurements of SCOs using Ca2+ imaging, ESA using microelectrode array (MEA) technology, and dendritic complexity using Sholl analysis were performed in primary murine cortical neurons from wild-type (WT) and/or ryanodine receptor 1 (RyR1T4826I/T4826I) mice between 5 and 14 d in vitro (DIV). [H3]ryanodine binding analysis and a single-channel voltage clamp were utilized to measure engagement of RyRs as a direct target of DM. Results: Neuronal networks responded to DM (30–70 nM) as early as 5 DIV, reducing SCO amplitude and depressing ESA and burst frequencies by 60–70%. DM (10–300 nM) enhanced axonal growth in a nonmonotonic manner. DM≥100 nM enhanced dendritic complexity. DM stabilized channel open states of RyR1, RyR2, and cortical preparations expressing all three isoforms. DM (30 nM) altered gating kinetics of RyR1 channels, increasing mean open time, decreasing mean closed time, and thereby enhancing overall open probability. SCO patterns from cortical networks expressing RyR1T4826I/T4826I were more responsive to DM than WT. RyR1T4826I/T4826I neurons showed inherently longer axonal lengths than WT neurons and maintained less length-promoting responses to nanomolar DM. Conclusions: Our findings suggested that RyRs were sensitive molecular targets of DM with functional consequences likely relevant for mediating abnormal neuronal network connectivity in vitro. https://doi.org/10.1289/EHP4583" @default.
• W2948465206 created "2019-06-14" @default.
• W2948465206 creator A5012677271 @default.
• W2948465206 creator A5019456473 @default.
• W2948465206 creator A5050388265 @default.
• W2948465206 creator A5062413385 @default.
• W2948465206 creator A5077475488 @default.
• W2948465206 creator A5079399944 @default.
• W2948465206 creator A5083780440 @default.
• W2948465206 creator A5086960113 @default.
• W2948465206 creator A5088059015 @default.
• W2948465206 date "2019-06-01" @default.
• W2948465206 modified "2023-10-13" @default.
• W2948465206 title "Influence of Nanomolar Deltamethrin on the Hallmarks of Primary Cultured Cortical Neuronal Network and the Role of Ryanodine Receptors" @default.
• W2948465206 cites W1604664180 @default.
• W2948465206 cites W1624422393 @default.
• W2948465206 cites W1650401181 @default.
• W2948465206 cites W1758898161 @default.
• W2948465206 cites W1843936429 @default.
• W2948465206 cites W1895213472 @default.
• W2948465206 cites W1963189968 @default.
• W2948465206 cites W1976263419 @default.
• W2948465206 cites W1983898705 @default.
• W2948465206 cites W1993393582 @default.
• W2948465206 cites W1994677962 @default.
• W2948465206 cites W1996362740 @default.
• W2948465206 cites W1997302222 @default.
• W2948465206 cites W1998502210 @default.
• W2948465206 cites W1998977808 @default.
• W2948465206 cites W2003298758 @default.
• W2948465206 cites W2006252642 @default.
• W2948465206 cites W2007918429 @default.
• W2948465206 cites W2009816497 @default.
• W2948465206 cites W2010455912 @default.
• W2948465206 cites W2011656452 @default.
• W2948465206 cites W2012528202 @default.
• W2948465206 cites W2012665183 @default.
• W2948465206 cites W2015667498 @default.
• W2948465206 cites W2018589585 @default.
• W2948465206 cites W2028338427 @default.
• W2948465206 cites W2030014404 @default.
• W2948465206 cites W2032223367 @default.
• W2948465206 cites W2032821794 @default.
• W2948465206 cites W2033705578 @default.
• W2948465206 cites W2035977066 @default.
• W2948465206 cites W2037200077 @default.
• W2948465206 cites W2038267430 @default.
• W2948465206 cites W2040311875 @default.
• W2948465206 cites W2047719630 @default.
• W2948465206 cites W2053813256 @default.
• W2948465206 cites W2057153652 @default.
• W2948465206 cites W2057727146 @default.
• W2948465206 cites W2057913992 @default.
• W2948465206 cites W2058528480 @default.
• W2948465206 cites W2059647854 @default.
• W2948465206 cites W2061353638 @default.
• W2948465206 cites W2061383447 @default.
• W2948465206 cites W2062825630 @default.
• W2948465206 cites W2067994732 @default.
• W2948465206 cites W2068004736 @default.
• W2948465206 cites W2068016566 @default.
• W2948465206 cites W2068315357 @default.
• W2948465206 cites W2069002937 @default.
• W2948465206 cites W2075984067 @default.
• W2948465206 cites W2075989491 @default.
• W2948465206 cites W2083514820 @default.
• W2948465206 cites W2083653260 @default.
• W2948465206 cites W2093575598 @default.
• W2948465206 cites W2096827547 @default.
• W2948465206 cites W2098896781 @default.
• W2948465206 cites W2108656984 @default.
• W2948465206 cites W2115457184 @default.
• W2948465206 cites W2116635624 @default.
• W2948465206 cites W2117689911 @default.
• W2948465206 cites W2123548738 @default.
• W2948465206 cites W2124174369 @default.
• W2948465206 cites W2125595393 @default.
• W2948465206 cites W2126425609 @default.
• W2948465206 cites W2129964885 @default.
• W2948465206 cites W2130186201 @default.
• W2948465206 cites W2140983699 @default.
• W2948465206 cites W2143798904 @default.
• W2948465206 cites W2145602839 @default.
• W2948465206 cites W2148574990 @default.
• W2948465206 cites W2155586061 @default.
• W2948465206 cites W2158808703 @default.
• W2948465206 cites W2167835032 @default.
• W2948465206 cites W2169931542 @default.
• W2948465206 cites W2171969201 @default.
• W2948465206 cites W2285667698 @default.
• W2948465206 cites W2293324340 @default.
• W2948465206 cites W2485250233 @default.
• W2948465206 cites W2521250091 @default.
• W2948465206 cites W2539485445 @default.
• W2948465206 cites W2546406070 @default.
• W2948465206 cites W2565322371 @default.
• W2948465206 cites W2568328160 @default.
• W2948465206 cites W2587770103 @default.

• next