FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2948748962> ?p ?o ?g. }

• W2948748962 abstract "Many bacteria enter the viable but non-culturable (VBNC) state to maximize resources and increase their tolerance to harmful conditions to cope with environmental stress, which has been described for a plethora of important human and foodborne pathogens. VBNC pathogens can potentially present a serious risk to human health as they are invisible to routine microbiological culture-based methods. Of high importance is the increased tolerance to antibiotics or disinfectant measures while in the VBNC state. The greatest remaining challenge for such investigations is the lack of an appropriate, cost-effective multi-species screening method due to experimental constraints. In this study, we investigated if de novo ATP production of cells in the VBNC state is a suitable indicator for overall cell viability that can be utilized to determine the minimum ATP inhibitory concentration (MAIC) of antibiotics and other antimicrobials. To validate this approach, heat-stress time-kill experiments were performed with both culturable and VBNC cells. We developed a comprehensive experimental setup and demonstrated the applicability of this VBNC-MIC assay for testing the tolerance of twelve strains of four important bacterial species (Escherichia coli, Bacillus cereus, Pseudomonas aeruginosa and Listeria monocytogenes) in the VBNC state to eight important antimicrobials including four different antibiotics. We confirmed that bacteria in the VBNC state were resistant to all tested antibiotics (ampicillin, imipenem, ciprofloxacin and gentamycin) and additionally insensitive to disinfectants (benzalkonium chloride and trioctylmethalammonium chloride) and preservatives (bronopol and sodium azide). These data emphasize the need for further research regarding the characteristics of bacterial pathogens in the VBNC state and present the advantages and high-throughput capabilities of ATP determinations to investigate tolerance of VBNC pathogens to antimicrobials. The presented method should be helpful in order to identify appropriate countermeasures, treatments or disinfectants when confronted with bacterial pathogens in the VBNC state." @default.
• W2948748962 created "2019-06-14" @default.
• W2948748962 creator A5022605685 @default.
• W2948748962 creator A5044833601 @default.
• W2948748962 creator A5044998045 @default.
• W2948748962 creator A5062653045 @default.
• W2948748962 creator A5079542403 @default.
• W2948748962 creator A5081473594 @default.
• W2948748962 date "2019-06-14" @default.
• W2948748962 modified "2023-10-05" @default.
• W2948748962 title "A Fast and Easy ATP-Based Approach Enables MIC Testing for Non-resuscitating VBNC Pathogens" @default.
• W2948748962 cites W1876310058 @default.
• W2948748962 cites W1969489218 @default.
• W2948748962 cites W1975910348 @default.
• W2948748962 cites W1980511941 @default.
• W2948748962 cites W1987447861 @default.
• W2948748962 cites W2004444860 @default.
• W2948748962 cites W2006530152 @default.
• W2948748962 cites W2039685044 @default.
• W2948748962 cites W2045825103 @default.
• W2948748962 cites W2047160440 @default.
• W2948748962 cites W2075307840 @default.
• W2948748962 cites W2076572182 @default.
• W2948748962 cites W2091902669 @default.
• W2948748962 cites W2095016906 @default.
• W2948748962 cites W2115172046 @default.
• W2948748962 cites W2125219078 @default.
• W2948748962 cites W2128912763 @default.
• W2948748962 cites W2132963724 @default.
• W2948748962 cites W2160654138 @default.
• W2948748962 cites W2168280500 @default.
• W2948748962 cites W2188845894 @default.
• W2948748962 cites W2206952615 @default.
• W2948748962 cites W2255500819 @default.
• W2948748962 cites W2301843929 @default.
• W2948748962 cites W2325167129 @default.
• W2948748962 cites W2336625825 @default.
• W2948748962 cites W2561407715 @default.
• W2948748962 cites W2562944419 @default.
• W2948748962 cites W2563986873 @default.
• W2948748962 cites W2584510934 @default.
• W2948748962 cites W2604977417 @default.
• W2948748962 cites W2625713836 @default.
• W2948748962 cites W2721522249 @default.
• W2948748962 cites W2771565742 @default.
• W2948748962 cites W2777170042 @default.
• W2948748962 cites W2782470314 @default.
• W2948748962 cites W2887056633 @default.
• W2948748962 cites W2888835006 @default.
• W2948748962 cites W2895427804 @default.
• W2948748962 cites W2522287331 @default.
• W2948748962 doi "https://doi.org/10.3389/fmicb.2019.01365" @default.
• W2948748962 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6587809" @default.
• W2948748962 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/31258524" @default.
• W2948748962 hasPublicationYear "2019" @default.
• W2948748962 type Work @default.
• W2948748962 sameAs 2948748962 @default.
• W2948748962 citedByCount "20" @default.
• W2948748962 countsByYear W29487489622019 @default.
• W2948748962 countsByYear W29487489622020 @default.
• W2948748962 countsByYear W29487489622021 @default.
• W2948748962 countsByYear W29487489622022 @default.
• W2948748962 countsByYear W29487489622023 @default.
• W2948748962 crossrefType "journal-article" @default.
• W2948748962 hasAuthorship W2948748962A5022605685 @default.
• W2948748962 hasAuthorship W2948748962A5044833601 @default.
• W2948748962 hasAuthorship W2948748962A5044998045 @default.
• W2948748962 hasAuthorship W2948748962A5062653045 @default.
• W2948748962 hasAuthorship W2948748962A5079542403 @default.
• W2948748962 hasAuthorship W2948748962A5081473594 @default.
• W2948748962 hasBestOaLocation W29487489621 @default.
• W2948748962 hasConcept C178790620 @default.
• W2948748962 hasConcept C185592680 @default.
• W2948748962 hasConcept C2775919038 @default.
• W2948748962 hasConcept C2776021129 @default.
• W2948748962 hasConcept C2777118263 @default.
• W2948748962 hasConcept C2778512257 @default.
• W2948748962 hasConcept C2781221799 @default.
• W2948748962 hasConcept C2781350384 @default.
• W2948748962 hasConcept C4937899 @default.
• W2948748962 hasConcept C501593827 @default.
• W2948748962 hasConcept C523546767 @default.
• W2948748962 hasConcept C54355233 @default.
• W2948748962 hasConcept C86803240 @default.
• W2948748962 hasConcept C89423630 @default.
• W2948748962 hasConceptScore W2948748962C178790620 @default.
• W2948748962 hasConceptScore W2948748962C185592680 @default.
• W2948748962 hasConceptScore W2948748962C2775919038 @default.
• W2948748962 hasConceptScore W2948748962C2776021129 @default.
• W2948748962 hasConceptScore W2948748962C2777118263 @default.
• W2948748962 hasConceptScore W2948748962C2778512257 @default.
• W2948748962 hasConceptScore W2948748962C2781221799 @default.
• W2948748962 hasConceptScore W2948748962C2781350384 @default.
• W2948748962 hasConceptScore W2948748962C4937899 @default.
• W2948748962 hasConceptScore W2948748962C501593827 @default.
• W2948748962 hasConceptScore W2948748962C523546767 @default.
• W2948748962 hasConceptScore W2948748962C54355233 @default.
• W2948748962 hasConceptScore W2948748962C86803240 @default.
• W2948748962 hasConceptScore W2948748962C89423630 @default.
• W2948748962 hasLocation W29487489621 @default.

• next