FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2949050970> ?p ?o ?g. }

• W2949050970 abstract "Abstract Evolution is often an obstacle to the engineering of stable biological systems due to the selection of mutations inactivating costly gene circuits. Gene overlaps induce important constraints on sequences and their evolution. We show that these constraints can be harnessed to increase the stability of synthetic circuits by purging loss-of-function mutations. We combine computational and synthetic biology approaches to rationally design an overlapping reading frame expressing an essential gene within an existing gene to protect. Our algorithm succeeded in creating overlapping reading frames in 80% of E. coli genes. Experimentally, scoring mutations in both genes of such overlapping construct, we found that a significant fraction of mutations impacting the gene to protect have a deleterious effect on the essential gene. Such an overlap thus protects a costly gene from removal by natural selection by associating the benefit of this removal with a larger or even lethal cost. In our synthetic constructs, the overlap converts many of the possible mutants into evolutionary dead-ends, effectively changing the fitness landscape and reducing the evolutionary potential of the system." @default.
• W2949050970 created "2019-06-14" @default.
• W2949050970 creator A5021545267 @default.
• W2949050970 creator A5036056507 @default.
• W2949050970 creator A5036551333 @default.
• W2949050970 creator A5047096952 @default.
• W2949050970 creator A5056996051 @default.
• W2949050970 creator A5062215861 @default.
• W2949050970 creator A5074810950 @default.
• W2949050970 date "2019-06-03" @default.
• W2949050970 modified "2023-10-17" @default.
• W2949050970 title "Engineering gene overlaps to sustain genetic constructs in vivo" @default.
• W2949050970 cites W1566245339 @default.
• W2949050970 cites W1590001370 @default.
• W2949050970 cites W1968752956 @default.
• W2949050970 cites W1978119727 @default.
• W2949050970 cites W1984874903 @default.
• W2949050970 cites W1988327779 @default.
• W2949050970 cites W1990585969 @default.
• W2949050970 cites W1990695168 @default.
• W2949050970 cites W1993712384 @default.
• W2949050970 cites W1994028898 @default.
• W2949050970 cites W2000989493 @default.
• W2949050970 cites W2003593714 @default.
• W2949050970 cites W2004361023 @default.
• W2949050970 cites W2008127844 @default.
• W2949050970 cites W2011343696 @default.
• W2949050970 cites W2019385975 @default.
• W2949050970 cites W2060969855 @default.
• W2949050970 cites W2066200885 @default.
• W2949050970 cites W2082161669 @default.
• W2949050970 cites W2092247774 @default.
• W2949050970 cites W2096286650 @default.
• W2949050970 cites W2096978472 @default.
• W2949050970 cites W2098390695 @default.
• W2949050970 cites W2112269135 @default.
• W2949050970 cites W2113422929 @default.
• W2949050970 cites W2122332608 @default.
• W2949050970 cites W2122686110 @default.
• W2949050970 cites W2133790733 @default.
• W2949050970 cites W2139566973 @default.
• W2949050970 cites W2139970361 @default.
• W2949050970 cites W2142391696 @default.
• W2949050970 cites W2149174733 @default.
• W2949050970 cites W2159573893 @default.
• W2949050970 cites W2165479490 @default.
• W2949050970 cites W2192893656 @default.
• W2949050970 cites W2279510465 @default.
• W2949050970 cites W2609066137 @default.
• W2949050970 cites W2790907951 @default.
• W2949050970 cites W2793677392 @default.
• W2949050970 cites W2900490866 @default.
• W2949050970 cites W3000068290 @default.
• W2949050970 cites W4243772627 @default.
• W2949050970 doi "https://doi.org/10.1101/659243" @default.
• W2949050970 hasPublicationYear "2019" @default.
• W2949050970 type Work @default.
• W2949050970 sameAs 2949050970 @default.
• W2949050970 citedByCount "3" @default.
• W2949050970 countsByYear W29490509702020 @default.
• W2949050970 countsByYear W29490509702021 @default.
• W2949050970 crossrefType "posted-content" @default.
• W2949050970 hasAuthorship W2949050970A5021545267 @default.
• W2949050970 hasAuthorship W2949050970A5036056507 @default.
• W2949050970 hasAuthorship W2949050970A5036551333 @default.
• W2949050970 hasAuthorship W2949050970A5047096952 @default.
• W2949050970 hasAuthorship W2949050970A5056996051 @default.
• W2949050970 hasAuthorship W2949050970A5062215861 @default.
• W2949050970 hasAuthorship W2949050970A5074810950 @default.
• W2949050970 hasBestOaLocation W29490509702 @default.
• W2949050970 hasConcept C104317684 @default.
• W2949050970 hasConcept C126042441 @default.
• W2949050970 hasConcept C14036430 @default.
• W2949050970 hasConcept C141231307 @default.
• W2949050970 hasConcept C143065580 @default.
• W2949050970 hasConcept C154945302 @default.
• W2949050970 hasConcept C191908910 @default.
• W2949050970 hasConcept C2910387474 @default.
• W2949050970 hasConcept C41008148 @default.
• W2949050970 hasConcept C501734568 @default.
• W2949050970 hasConcept C54355233 @default.
• W2949050970 hasConcept C70721500 @default.
• W2949050970 hasConcept C7386963 @default.
• W2949050970 hasConcept C75268714 @default.
• W2949050970 hasConcept C76155785 @default.
• W2949050970 hasConcept C81917197 @default.
• W2949050970 hasConcept C86803240 @default.
• W2949050970 hasConceptScore W2949050970C104317684 @default.
• W2949050970 hasConceptScore W2949050970C126042441 @default.
• W2949050970 hasConceptScore W2949050970C14036430 @default.
• W2949050970 hasConceptScore W2949050970C141231307 @default.
• W2949050970 hasConceptScore W2949050970C143065580 @default.
• W2949050970 hasConceptScore W2949050970C154945302 @default.
• W2949050970 hasConceptScore W2949050970C191908910 @default.
• W2949050970 hasConceptScore W2949050970C2910387474 @default.
• W2949050970 hasConceptScore W2949050970C41008148 @default.
• W2949050970 hasConceptScore W2949050970C501734568 @default.
• W2949050970 hasConceptScore W2949050970C54355233 @default.
• W2949050970 hasConceptScore W2949050970C70721500 @default.
• W2949050970 hasConceptScore W2949050970C7386963 @default.

• next