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- W2949115954 abstract "Serum samples of 19 white-fronted brown lemurs (Eulemur fulvus albifrons), 7 ring-tailed lemurs (Lemur catta), 19 rhesus monkeys (Macaca mulatta), 24 squirrel monkeys (Saimiri sp.) and 10 other New world monkeys were collected and tested for antibodies against T. gondii by Sabin-Feldman dye test (SFT) and enzyme-linked immunosorbent assay (ELISA). Using SFT positive and SFT negative sera from white-fronted brown lemurs as serumcontrols an IgM ELISA, an IgG ELISA and an IgG avidity ELISA to detect antibodies against T. gondii was established. At assessment of ELISA a comparison with the results of SFT occured which is the golden standard for testing human sera for antibodies against T. gondii. In white-fronted brown lemurs and ring-tailed lemurs the assessments of serumsamples as positive or negative by SFT and IgG ELISA agreed to a large extent. There were different results for the squirrel monkeys, the cause of which could not be resolved. There were no results for the rhesus monkeys by SFT. For these animals similar serum titrations to white-fronted brown lemurs and ring-tailed lemurs arised by IgG ELISA – the youngest rhesus monkeys showed the lowest index values. In one ring-tailed lemur tissue cysts of T. gondii were demonstrated post mortem by histological examination. Because of this the results found by SFT and IgG ELISA for this animal were proved to be true and the suitability of IgG ELISA for testing sera from lemurs for antibodies against T. gondii was demonstrated. To examine the course of T. gondii infection only the IgG avidity ELISA was used, because the IgM ELISA proved to be unsuitable. In IgG avidity ELISA the greatest differences between test series were detected in squirrel monkeys and rhesus monkeys, this suggested that there was an early course of T. gondii infection in these animals." @default.
- W2949115954 created "2019-06-27" @default.
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- W2949115954 date "2006-01-01" @default.
- W2949115954 modified "2023-09-27" @default.
- W2949115954 title "Serologische Untersuchungen zur Toxoplasmose nicht humaner Primaten" @default.
- W2949115954 hasPublicationYear "2006" @default.
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