FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2949203582> ?p ?o ?g. }

• W2949203582 endingPage "e0211400" @default.
• W2949203582 startingPage "e0211400" @default.
• W2949203582 abstract "Archival tissues represent a rich resource for clinical genomic studies, particularly when coupled with comprehensive medical records. Use of these in next generation sequencing (NGS) is a priority. Nine formalin-fixed paraffin-embedded (FFPE) DNA extraction methods were evaluated using twelve FFPE samples of varying tissue types. Quality assessment included total yield, percent dsDNA, fragment analysis and multiplex PCR. After assessment, three tissue types from four FFPE DNA methods were selected for NGS downstream evaluation, targeted and whole exome sequencing. In addition, two low input library protocols were evaluated for WES. Analysis revealed average coverage across the target regions for WES was ~20-30X for all four FFPE DNA extraction methods. For the targeted panels, the highest molecular tag coverage was obtained with the Kingfisher FFPE extraction method. The genotype concordance was 99% for the commonly called variant positions between all four extraction methods with the targeted PCR NGS panel and 96% with WES. Assessing quality of extracted DNA aids in selecting the optimal NGS approach, and the choice of both DNA extraction and library preparation approaches can impact the performance of archival tissue in NGS." @default.
• W2949203582 created "2019-06-27" @default.
• W2949203582 creator A5004264491 @default.
• W2949203582 creator A5008375671 @default.
• W2949203582 creator A5027750899 @default.
• W2949203582 creator A5035310776 @default.
• W2949203582 creator A5037834422 @default.
• W2949203582 creator A5053182366 @default.
• W2949203582 creator A5067865294 @default.
• W2949203582 creator A5084097040 @default.
• W2949203582 date "2019-04-11" @default.
• W2949203582 modified "2023-10-01" @default.
• W2949203582 title "Use of FFPE-derived DNA in next generation sequencing: DNA extraction methods" @default.
• W2949203582 cites W1041941258 @default.
• W2949203582 cites W152953340 @default.
• W2949203582 cites W1910830047 @default.
• W2949203582 cites W1973036362 @default.
• W2949203582 cites W2013367562 @default.
• W2949203582 cites W2017320236 @default.
• W2949203582 cites W2026247770 @default.
• W2949203582 cites W2038484593 @default.
• W2949203582 cites W2076865561 @default.
• W2949203582 cites W2083577779 @default.
• W2949203582 cites W2085334462 @default.
• W2949203582 cites W2088683935 @default.
• W2949203582 cites W2101248947 @default.
• W2949203582 cites W2103441770 @default.
• W2949203582 cites W2116121073 @default.
• W2949203582 cites W2119180969 @default.
• W2949203582 cites W2124649685 @default.
• W2949203582 cites W2126665685 @default.
• W2949203582 cites W2128016314 @default.
• W2949203582 cites W2135401810 @default.
• W2949203582 cites W2154448561 @default.
• W2949203582 cites W2156918898 @default.
• W2949203582 cites W2517216747 @default.
• W2949203582 cites W2521999166 @default.
• W2949203582 cites W2564452722 @default.
• W2949203582 cites W2613808212 @default.
• W2949203582 cites W2796445892 @default.
• W2949203582 cites W2802627407 @default.
• W2949203582 cites W35335073 @default.
• W2949203582 cites W4239366021 @default.
• W2949203582 doi "https://doi.org/10.1371/journal.pone.0211400" @default.
• W2949203582 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6459541" @default.
• W2949203582 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30973937" @default.
• W2949203582 hasPublicationYear "2019" @default.
• W2949203582 type Work @default.
• W2949203582 sameAs 2949203582 @default.
• W2949203582 citedByCount "51" @default.
• W2949203582 countsByYear W29492035822019 @default.
• W2949203582 countsByYear W29492035822020 @default.
• W2949203582 countsByYear W29492035822021 @default.
• W2949203582 countsByYear W29492035822022 @default.
• W2949203582 countsByYear W29492035822023 @default.
• W2949203582 crossrefType "journal-article" @default.
• W2949203582 hasAuthorship W2949203582A5004264491 @default.
• W2949203582 hasAuthorship W2949203582A5008375671 @default.
• W2949203582 hasAuthorship W2949203582A5027750899 @default.
• W2949203582 hasAuthorship W2949203582A5035310776 @default.
• W2949203582 hasAuthorship W2949203582A5037834422 @default.
• W2949203582 hasAuthorship W2949203582A5053182366 @default.
• W2949203582 hasAuthorship W2949203582A5067865294 @default.
• W2949203582 hasAuthorship W2949203582A5084097040 @default.
• W2949203582 hasBestOaLocation W29492035821 @default.
• W2949203582 hasConcept C104317684 @default.
• W2949203582 hasConcept C10590036 @default.
• W2949203582 hasConcept C160798450 @default.
• W2949203582 hasConcept C16671776 @default.
• W2949203582 hasConcept C17757408 @default.
• W2949203582 hasConcept C2781188995 @default.
• W2949203582 hasConcept C49105822 @default.
• W2949203582 hasConcept C501734568 @default.
• W2949203582 hasConcept C51679486 @default.
• W2949203582 hasConcept C54355233 @default.
• W2949203582 hasConcept C552990157 @default.
• W2949203582 hasConcept C60644358 @default.
• W2949203582 hasConcept C70721500 @default.
• W2949203582 hasConcept C78063203 @default.
• W2949203582 hasConcept C86803240 @default.
• W2949203582 hasConcept C90583042 @default.
• W2949203582 hasConceptScore W2949203582C104317684 @default.
• W2949203582 hasConceptScore W2949203582C10590036 @default.
• W2949203582 hasConceptScore W2949203582C160798450 @default.
• W2949203582 hasConceptScore W2949203582C16671776 @default.
• W2949203582 hasConceptScore W2949203582C17757408 @default.
• W2949203582 hasConceptScore W2949203582C2781188995 @default.
• W2949203582 hasConceptScore W2949203582C49105822 @default.
• W2949203582 hasConceptScore W2949203582C501734568 @default.
• W2949203582 hasConceptScore W2949203582C51679486 @default.
• W2949203582 hasConceptScore W2949203582C54355233 @default.
• W2949203582 hasConceptScore W2949203582C552990157 @default.
• W2949203582 hasConceptScore W2949203582C60644358 @default.
• W2949203582 hasConceptScore W2949203582C70721500 @default.
• W2949203582 hasConceptScore W2949203582C78063203 @default.
• W2949203582 hasConceptScore W2949203582C86803240 @default.
• W2949203582 hasConceptScore W2949203582C90583042 @default.
• W2949203582 hasIssue "4" @default.

• next