FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2949206575> ?p ?o ?g. }

• W2949206575 endingPage "785" @default.
• W2949206575 startingPage "784" @default.
• W2949206575 abstract "Background: AML is the most common malignant myeloid disorder in adults. Relapses are initiated by chemoresistant leukemic cells. DNA damage and repair mechanisms influence not only the genetic predisposition to leukemia but are also very important for refractoriness to treatment. Aims: The aim of this study was to investigate the possible alterations in the gene expression profile in DNA damage signaling pathways and apoptosis in two leukemic cell lines following their exposure to chemotherapeutic agents, idarubicin and cytarabine and to confirm the results in AML patients’ cells. Methods: Kasumi-1 and MV4-11 AML cells were treated with either idarubicin (0.1 μM) for 6 h or cytarabine (1 μM) for 48 h. Dead cells were eliminated after exposure to the drug using the appropriate commercial kit. Gene expression profiling through PCR arrays analysis (RT2Profiler, Qiagen) was performed after RNA extraction from untreated, drug-treated and chemoresistant (live) cells. Human DNA Damage Signaling pathway related genes’ expression was evaluated and analyzed through RT2Profiler PCR Array data analysis tool. Following our initial results, PPP1R15A gene's relative expression was evaluated by qRT-PCR analysis with QuantiTect Primer Assays kit (Qiagen) in 12 de novo AML patients before the onset of the 7 + 3 combination chemotherapy and 17 healthy donors using the 2^-ΔΔCt method. Results: PCR Array analysis after idarubicin treatment of Kasumi-1 cells revealed a significant up-regulation of genes involved in apoptosis (BBC3), cell cycle (CDKN1A, PPP1R15A), DNA damage and repair (PNP), and ATM/ATR signaling (RBBP8) while cytarabine treatment led to the up-regulation of genes involved mostly in the DSB repair pathway i.e. HUS1,MLH1,NBN,XRCC1,XRCC2). Interestingly, significant differences in their gene expression patterns were observed between cytarabine-treated Kasumi-1 cells and chemoresistant ones. HUS-1 gene (DSB) was up-regulated in cytarabine-treated cells and down-regulated in chemoresistant cells, while MLH1 and NBN genes presented the opposite pattern. Most importantly, PPP1R15A gene's expression in both cytarabine and idarubicin chemoresistant MV4-11 cells was significantly up-regulated compared to drug treated cells. PPP1R15A gene's relative expression was significantly up-regulated in AML patients’ cells compared to controls (median: 0.72 vs 1.72, p < 0.05) and in non-responding to chemotherapy AML patients compared to controls (median: 0.72 vs 1.21, p < 0.05). Summary/Conclusion: Our data suggest differences in the gene expression pattern between chemoresistant cells and drug-treated cells, indicating the significance of DNA damage and repair pathways involved in chemoresistance. Specifically, the up-regulation of PPP1R15A gene in chemoresistant MV4-11 cells after treatment with both agents is of great importance since this gene participates in cell cycle and its transcript levels are increased following stressful growth arrest and treatment with DNA-damaging agents. Considering that MV4-11 is an AML chemoresistant cell line, up-regulation of the expression of PPP1R15A gene which facilitates recovery of cells from stress is totally compatible. These findings were further verified by qRT-PCR demonstrating overexpression of PPP1R15A gene in AML chemoresistant patients indicating the involvement of this gene in chemoresistance mechanisms." @default.
• W2949206575 created "2019-06-27" @default.
• W2949206575 creator A5004716597 @default.
• W2949206575 creator A5013439164 @default.
• W2949206575 creator A5023609943 @default.
• W2949206575 creator A5029656734 @default.
• W2949206575 creator A5032351167 @default.
• W2949206575 creator A5033425321 @default.
• W2949206575 creator A5048761931 @default.
• W2949206575 creator A5058783272 @default.
• W2949206575 creator A5063015209 @default.
• W2949206575 creator A5066644658 @default.
• W2949206575 creator A5073757935 @default.
• W2949206575 creator A5082219280 @default.
• W2949206575 date "2019-06-01" @default.
• W2949206575 modified "2023-10-16" @default.
• W2949206575 title "PB1703 PPP1R15A GENE IS OVEREXPRESSED IN CHEMORESISTANT ACUTE MYELOID LEUKEMIA (AML)" @default.
• W2949206575 doi "https://doi.org/10.1097/01.hs9.0000565328.28450.f4" @default.
• W2949206575 hasPublicationYear "2019" @default.
• W2949206575 type Work @default.
• W2949206575 sameAs 2949206575 @default.
• W2949206575 citedByCount "0" @default.
• W2949206575 crossrefType "journal-article" @default.
• W2949206575 hasAuthorship W2949206575A5004716597 @default.
• W2949206575 hasAuthorship W2949206575A5013439164 @default.
• W2949206575 hasAuthorship W2949206575A5023609943 @default.
• W2949206575 hasAuthorship W2949206575A5029656734 @default.
• W2949206575 hasAuthorship W2949206575A5032351167 @default.
• W2949206575 hasAuthorship W2949206575A5033425321 @default.
• W2949206575 hasAuthorship W2949206575A5048761931 @default.
• W2949206575 hasAuthorship W2949206575A5058783272 @default.
• W2949206575 hasAuthorship W2949206575A5063015209 @default.
• W2949206575 hasAuthorship W2949206575A5066644658 @default.
• W2949206575 hasAuthorship W2949206575A5073757935 @default.
• W2949206575 hasAuthorship W2949206575A5082219280 @default.
• W2949206575 hasBestOaLocation W29492065751 @default.
• W2949206575 hasConcept C104317684 @default.
• W2949206575 hasConcept C134935766 @default.
• W2949206575 hasConcept C143425029 @default.
• W2949206575 hasConcept C150194340 @default.
• W2949206575 hasConcept C153911025 @default.
• W2949206575 hasConcept C18431079 @default.
• W2949206575 hasConcept C203014093 @default.
• W2949206575 hasConcept C2778041864 @default.
• W2949206575 hasConcept C2778461978 @default.
• W2949206575 hasConcept C2778729363 @default.
• W2949206575 hasConcept C2779117419 @default.
• W2949206575 hasConcept C2779282312 @default.
• W2949206575 hasConcept C502942594 @default.
• W2949206575 hasConcept C54355233 @default.
• W2949206575 hasConcept C552990157 @default.
• W2949206575 hasConcept C71924100 @default.
• W2949206575 hasConcept C86803240 @default.
• W2949206575 hasConceptScore W2949206575C104317684 @default.
• W2949206575 hasConceptScore W2949206575C134935766 @default.
• W2949206575 hasConceptScore W2949206575C143425029 @default.
• W2949206575 hasConceptScore W2949206575C150194340 @default.
• W2949206575 hasConceptScore W2949206575C153911025 @default.
• W2949206575 hasConceptScore W2949206575C18431079 @default.
• W2949206575 hasConceptScore W2949206575C203014093 @default.
• W2949206575 hasConceptScore W2949206575C2778041864 @default.
• W2949206575 hasConceptScore W2949206575C2778461978 @default.
• W2949206575 hasConceptScore W2949206575C2778729363 @default.
• W2949206575 hasConceptScore W2949206575C2779117419 @default.
• W2949206575 hasConceptScore W2949206575C2779282312 @default.
• W2949206575 hasConceptScore W2949206575C502942594 @default.
• W2949206575 hasConceptScore W2949206575C54355233 @default.
• W2949206575 hasConceptScore W2949206575C552990157 @default.
• W2949206575 hasConceptScore W2949206575C71924100 @default.
• W2949206575 hasConceptScore W2949206575C86803240 @default.
• W2949206575 hasIssue "S1" @default.
• W2949206575 hasLocation W29492065751 @default.
• W2949206575 hasLocation W29492065752 @default.
• W2949206575 hasOpenAccess W2949206575 @default.
• W2949206575 hasPrimaryLocation W29492065751 @default.
• W2949206575 hasRelatedWork W1583942422 @default.
• W2949206575 hasRelatedWork W1913413628 @default.
• W2949206575 hasRelatedWork W2023291141 @default.
• W2949206575 hasRelatedWork W2048294528 @default.
• W2949206575 hasRelatedWork W2124233789 @default.
• W2949206575 hasRelatedWork W2534464466 @default.
• W2949206575 hasRelatedWork W289820089 @default.
• W2949206575 hasRelatedWork W29086519 @default.
• W2949206575 hasRelatedWork W3602028 @default.
• W2949206575 hasRelatedWork W4245792878 @default.
• W2949206575 hasVolume "3" @default.
• W2949206575 isParatext "false" @default.
• W2949206575 isRetracted "false" @default.
• W2949206575 magId "2949206575" @default.
• W2949206575 workType "article" @default.