FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2949486048> ?p ?o ?g. }

• W2949486048 endingPage "211" @default.
• W2949486048 startingPage "201" @default.
• W2949486048 abstract "To assess whether an adenoviral vector carrying the bone morphogenetic protein genes (Ad.BMP-2) can transduce human muscle tissue and direct it toward osteogenic differentiation within one hour.This in vitro study, performed at the Department of Molecular Biology, Faculty of Science, Zagreb from 2012 to 2017, used human muscle tissue samples collected during anterior cruciate ligament reconstructions performed in St Catherine Hospital, Zabok. Samples from 28 patients were transduced with adenoviral vector carrying firefly luciferase cDNA (Ad.luc) by using different doses and times of transduction, and with addition of positive ions for transduction enhancement. The optimized protocol was further tested on muscle samples from three new patients, which were transduced with Ad.BMP-2. Released bone morphogenetic protein 2 (BMP-2) levels in osteogenic medium were measured every three days during a period of 21 days. Expression of osteogenic markers was measured at day 14 and 21. After 21 days of cultivation, muscle tissue was immunohistochemically stained for collagen type I detection (COL-I).The new transduction protocol was established using 108 plaque-forming units (P<0.001) as an optimal dose of adenoviral vector and 30 minutes (P<0.001) as an optimal contact time. Positive ions did not enhance transduction. Samples transduced with Ad.BMP-2 according to the optimized protocol showed enhanced expression of osteogenic markers (P<0.050), BMP-2 (P<0.001), and COL I.This study confirms that Ad.BMP-2 can transduce human muscle tissue and direct it toward osteogenic differentiation within 30 minutes." @default.
• W2949486048 created "2019-06-27" @default.
• W2949486048 creator A5017159217 @default.
• W2949486048 creator A5034936703 @default.
• W2949486048 creator A5039478872 @default.
• W2949486048 creator A5046739154 @default.
• W2949486048 creator A5058935968 @default.
• W2949486048 creator A5076291391 @default.
• W2949486048 creator A5076388613 @default.
• W2949486048 creator A5087188974 @default.
• W2949486048 creator A5087647385 @default.
• W2949486048 date "2019-06-01" @default.
• W2949486048 modified "2023-09-27" @default.
• W2949486048 title "Optimization of an ex vivo gene transfer to the hamstrings tendons muscle remnants: potential for genetic enhancement of bone healing" @default.
• W2949486048 cites W1530066186 @default.
• W2949486048 cites W1616824889 @default.
• W2949486048 cites W1865442119 @default.
• W2949486048 cites W1971409892 @default.
• W2949486048 cites W1973811358 @default.
• W2949486048 cites W1977211871 @default.
• W2949486048 cites W2001719381 @default.
• W2949486048 cites W2014362480 @default.
• W2949486048 cites W2019777614 @default.
• W2949486048 cites W2019981271 @default.
• W2949486048 cites W2022907036 @default.
• W2949486048 cites W2025592015 @default.
• W2949486048 cites W2032807060 @default.
• W2949486048 cites W2042474003 @default.
• W2949486048 cites W2065885294 @default.
• W2949486048 cites W2066050937 @default.
• W2949486048 cites W2067048467 @default.
• W2949486048 cites W2069906452 @default.
• W2949486048 cites W2089863521 @default.
• W2949486048 cites W2090831878 @default.
• W2949486048 cites W2102032120 @default.
• W2949486048 cites W2104779362 @default.
• W2949486048 cites W2129904461 @default.
• W2949486048 cites W2139055053 @default.
• W2949486048 cites W2141412603 @default.
• W2949486048 cites W2202022736 @default.
• W2949486048 cites W2202603970 @default.
• W2949486048 cites W2217100198 @default.
• W2949486048 cites W2319534054 @default.
• W2949486048 cites W2328558212 @default.
• W2949486048 cites W2381411745 @default.
• W2949486048 cites W2399850257 @default.
• W2949486048 cites W2557793289 @default.
• W2949486048 cites W2581438227 @default.
• W2949486048 cites W2608335129 @default.
• W2949486048 cites W2749409163 @default.
• W2949486048 cites W3102131143 @default.
• W2949486048 cites W3102414871 @default.
• W2949486048 doi "https://doi.org/10.3325/cmj.2019.60.201" @default.
• W2949486048 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6563169" @default.
• W2949486048 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/31187947" @default.
• W2949486048 hasPublicationYear "2019" @default.
• W2949486048 type Work @default.
• W2949486048 sameAs 2949486048 @default.
• W2949486048 citedByCount "0" @default.
• W2949486048 crossrefType "journal-article" @default.
• W2949486048 hasAuthorship W2949486048A5017159217 @default.
• W2949486048 hasAuthorship W2949486048A5034936703 @default.
• W2949486048 hasAuthorship W2949486048A5039478872 @default.
• W2949486048 hasAuthorship W2949486048A5046739154 @default.
• W2949486048 hasAuthorship W2949486048A5058935968 @default.
• W2949486048 hasAuthorship W2949486048A5076291391 @default.
• W2949486048 hasAuthorship W2949486048A5076388613 @default.
• W2949486048 hasAuthorship W2949486048A5087188974 @default.
• W2949486048 hasAuthorship W2949486048A5087647385 @default.
• W2949486048 hasBestOaLocation W29494860481 @default.
• W2949486048 hasConcept C104317684 @default.
• W2949486048 hasConcept C105702510 @default.
• W2949486048 hasConcept C111335760 @default.
• W2949486048 hasConcept C111599444 @default.
• W2949486048 hasConcept C136229726 @default.
• W2949486048 hasConcept C15152581 @default.
• W2949486048 hasConcept C153911025 @default.
• W2949486048 hasConcept C202751555 @default.
• W2949486048 hasConcept C207001950 @default.
• W2949486048 hasConcept C26291073 @default.
• W2949486048 hasConcept C2778514504 @default.
• W2949486048 hasConcept C30352789 @default.
• W2949486048 hasConcept C31507581 @default.
• W2949486048 hasConcept C49892992 @default.
• W2949486048 hasConcept C54009773 @default.
• W2949486048 hasConcept C54355233 @default.
• W2949486048 hasConcept C55493867 @default.
• W2949486048 hasConcept C71924100 @default.
• W2949486048 hasConcept C86803240 @default.
• W2949486048 hasConcept C95444343 @default.
• W2949486048 hasConceptScore W2949486048C104317684 @default.
• W2949486048 hasConceptScore W2949486048C105702510 @default.
• W2949486048 hasConceptScore W2949486048C111335760 @default.
• W2949486048 hasConceptScore W2949486048C111599444 @default.
• W2949486048 hasConceptScore W2949486048C136229726 @default.
• W2949486048 hasConceptScore W2949486048C15152581 @default.
• W2949486048 hasConceptScore W2949486048C153911025 @default.
• W2949486048 hasConceptScore W2949486048C202751555 @default.

• next