FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2949664166> ?p ?o ?g. }

• W2949664166 endingPage "e1007775" @default.
• W2949664166 startingPage "e1007775" @default.
• W2949664166 abstract "Toxoplasma gondii is an apicomplexan parasite with the ability to use foodborne, zoonotic, and congenital routes of transmission that causes severe disease in immunocompromised patients. The parasites harbor a lysosome-like organelle, termed the Vacuolar Compartment/Plant-Like Vacuole (VAC/PLV), which plays an important role in maintaining the lytic cycle and virulence of T. gondii. The VAC supplies proteolytic enzymes that contribute to the maturation of invasion effectors and that digest autophagosomes and endocytosed host proteins. Previous work identified a T. gondii ortholog of the Plasmodium falciparum chloroquine resistance transporter (PfCRT) that localized to the VAC. Here, we show that TgCRT is a membrane transporter that is functionally similar to PfCRT. We also genetically ablate TgCRT and reveal that the TgCRT protein plays a key role in maintaining the integrity of the parasite’s endolysosomal system by controlling morphology of the VAC. When TgCRT is absent, the VAC dramatically increases in volume by ~15-fold and overlaps with adjacent endosome-like compartments. Presumably to reduce aberrant swelling, transcription and translation of endolysosomal proteases are decreased in ΔTgCRT parasites. Expression of subtilisin protease 1 is significantly reduced, which impedes trimming of microneme proteins, and significantly decreases parasite invasion. Chemical or genetic inhibition of proteolysis within the VAC reverses these effects, reducing VAC size and partially restoring integrity of the endolysosomal system, microneme protein trimming, and invasion. Taken together, these findings reveal for the first time a physiological role of TgCRT in substrate transport that impacts VAC volume and the integrity of the endolysosomal system in T. gondii." @default.
• W2949664166 created "2019-06-27" @default.
• W2949664166 creator A5004692201 @default.
• W2949664166 creator A5022780309 @default.
• W2949664166 creator A5023965304 @default.
• W2949664166 creator A5025057384 @default.
• W2949664166 creator A5025333181 @default.
• W2949664166 creator A5034392777 @default.
• W2949664166 creator A5043921376 @default.
• W2949664166 creator A5046424130 @default.
• W2949664166 creator A5077598192 @default.
• W2949664166 creator A5086870303 @default.
• W2949664166 creator A5091781696 @default.
• W2949664166 date "2019-06-06" @default.
• W2949664166 modified "2023-10-01" @default.
• W2949664166 title "An ortholog of Plasmodium falciparum chloroquine resistance transporter (PfCRT) plays a key role in maintaining the integrity of the endolysosomal system in Toxoplasma gondii to facilitate host invasion" @default.
• W2949664166 cites W1554488048 @default.
• W2949664166 cites W1949622181 @default.
• W2949664166 cites W1963051611 @default.
• W2949664166 cites W1965804686 @default.
• W2949664166 cites W1967852756 @default.
• W2949664166 cites W1973725057 @default.
• W2949664166 cites W1975200363 @default.
• W2949664166 cites W1975302732 @default.
• W2949664166 cites W1977724107 @default.
• W2949664166 cites W2024791353 @default.
• W2949664166 cites W2027835262 @default.
• W2949664166 cites W2030165969 @default.
• W2949664166 cites W2032760765 @default.
• W2949664166 cites W2033725939 @default.
• W2949664166 cites W2037350416 @default.
• W2949664166 cites W2037832469 @default.
• W2949664166 cites W2038731675 @default.
• W2949664166 cites W2045140728 @default.
• W2949664166 cites W2045714098 @default.
• W2949664166 cites W2055527040 @default.
• W2949664166 cites W2070763276 @default.
• W2949664166 cites W2071920845 @default.
• W2949664166 cites W2072843074 @default.
• W2949664166 cites W2074450319 @default.
• W2949664166 cites W2079745747 @default.
• W2949664166 cites W2081323707 @default.
• W2949664166 cites W2092821995 @default.
• W2949664166 cites W2095352319 @default.
• W2949664166 cites W2098935280 @default.
• W2949664166 cites W2109626757 @default.
• W2949664166 cites W2114049739 @default.
• W2949664166 cites W2126516116 @default.
• W2949664166 cites W2126706705 @default.
• W2949664166 cites W2127056225 @default.
• W2949664166 cites W2137547491 @default.
• W2949664166 cites W2138865920 @default.
• W2949664166 cites W2141592854 @default.
• W2949664166 cites W2143687466 @default.
• W2949664166 cites W2145728129 @default.
• W2949664166 cites W2147828217 @default.
• W2949664166 cites W2158950061 @default.
• W2949664166 cites W2164898121 @default.
• W2949664166 cites W2167279371 @default.
• W2949664166 cites W2168420262 @default.
• W2949664166 cites W2172177549 @default.
• W2949664166 cites W2335425519 @default.
• W2949664166 cites W2376584008 @default.
• W2949664166 cites W2411068755 @default.
• W2949664166 cites W2565958256 @default.
• W2949664166 cites W2677736497 @default.
• W2949664166 cites W2756285626 @default.
• W2949664166 cites W2789672216 @default.
• W2949664166 cites W2889803626 @default.
• W2949664166 doi "https://doi.org/10.1371/journal.ppat.1007775" @default.
• W2949664166 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6553793" @default.
• W2949664166 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/31170269" @default.
• W2949664166 hasPublicationYear "2019" @default.
• W2949664166 type Work @default.
• W2949664166 sameAs 2949664166 @default.
• W2949664166 citedByCount "18" @default.
• W2949664166 countsByYear W29496641662019 @default.
• W2949664166 countsByYear W29496641662020 @default.
• W2949664166 countsByYear W29496641662021 @default.
• W2949664166 countsByYear W29496641662022 @default.
• W2949664166 countsByYear W29496641662023 @default.
• W2949664166 crossrefType "journal-article" @default.
• W2949664166 hasAuthorship W2949664166A5004692201 @default.
• W2949664166 hasAuthorship W2949664166A5022780309 @default.
• W2949664166 hasAuthorship W2949664166A5023965304 @default.
• W2949664166 hasAuthorship W2949664166A5025057384 @default.
• W2949664166 hasAuthorship W2949664166A5025333181 @default.
• W2949664166 hasAuthorship W2949664166A5034392777 @default.
• W2949664166 hasAuthorship W2949664166A5043921376 @default.
• W2949664166 hasAuthorship W2949664166A5046424130 @default.
• W2949664166 hasAuthorship W2949664166A5077598192 @default.
• W2949664166 hasAuthorship W2949664166A5086870303 @default.
• W2949664166 hasAuthorship W2949664166A5091781696 @default.
• W2949664166 hasBestOaLocation W29496641661 @default.
• W2949664166 hasConcept C102747710 @default.
• W2949664166 hasConcept C159654299 @default.
• W2949664166 hasConcept C170493617 @default.
• W2949664166 hasConcept C203014093 @default.

• next