Matches in SemOpenAlex for { <https://semopenalex.org/work/W2949672039> ?p ?o ?g. }
- W2949672039 abstract "The organisation of mammalian genomes into loops and topologically associating domains (TADs) contributes to chromatin structure, gene expression and recombination. TADs and many loops are formed by cohesin and positioned by CTCF. In proliferating cells, cohesin also mediates sister chromatid cohesion, which is essential for chromosome segregation. Current models of chromatin folding and cohesion are based on assumptions of how many cohesin and CTCF molecules organise the genome. Here we have measured absolute copy numbers and dynamics of cohesin, CTCF, NIPBL, WAPL and sororin by mass spectrometry, fluorescence-correlation spectroscopy and fluorescence recovery after photobleaching in HeLa cells. In G1-phase, there are ~250,000 nuclear cohesin complexes, of which ~ 160,000 are chromatin-bound. Comparison with chromatin immunoprecipitation-sequencing data implies that some genomic cohesin and CTCF enrichment sites are unoccupied in single cells at any one time. We discuss the implications of these findings for how cohesin can contribute to genome organisation and cohesion." @default.
- W2949672039 created "2019-06-27" @default.
- W2949672039 creator A5003103218 @default.
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- W2949672039 creator A5091479497 @default.
- W2949672039 date "2019-06-17" @default.
- W2949672039 modified "2023-10-12" @default.
- W2949672039 title "Absolute quantification of cohesin, CTCF and their regulators in human cells" @default.
- W2949672039 cites W1831401007 @default.
- W2949672039 cites W1873772354 @default.
- W2949672039 cites W1941076503 @default.
- W2949672039 cites W1952201404 @default.
- W2949672039 cites W1965863160 @default.
- W2949672039 cites W1968126063 @default.
- W2949672039 cites W1972307085 @default.
- W2949672039 cites W1973062929 @default.
- W2949672039 cites W1974930724 @default.
- W2949672039 cites W1978397604 @default.
- W2949672039 cites W1978696228 @default.
- W2949672039 cites W1980639342 @default.
- W2949672039 cites W1992225546 @default.
- W2949672039 cites W2000068315 @default.
- W2949672039 cites W2000548576 @default.
- W2949672039 cites W2001218098 @default.
- W2949672039 cites W2002832176 @default.
- W2949672039 cites W2002954217 @default.
- W2949672039 cites W2003101935 @default.
- W2949672039 cites W2003673194 @default.
- W2949672039 cites W2004304977 @default.
- W2949672039 cites W2013035015 @default.
- W2949672039 cites W2014012985 @default.
- W2949672039 cites W2019077723 @default.
- W2949672039 cites W2021080331 @default.
- W2949672039 cites W2022431828 @default.
- W2949672039 cites W2028022357 @default.
- W2949672039 cites W2032140335 @default.
- W2949672039 cites W2034040669 @default.
- W2949672039 cites W2040727271 @default.
- W2949672039 cites W2041182908 @default.
- W2949672039 cites W2042339569 @default.
- W2949672039 cites W2047005748 @default.
- W2949672039 cites W2048854522 @default.
- W2949672039 cites W2049754076 @default.
- W2949672039 cites W2053943711 @default.
- W2949672039 cites W2063450668 @default.
- W2949672039 cites W2065455422 @default.
- W2949672039 cites W2065836636 @default.
- W2949672039 cites W2070021921 @default.
- W2949672039 cites W2087934409 @default.
- W2949672039 cites W2097363543 @default.
- W2949672039 cites W2101129064 @default.
- W2949672039 cites W2106376891 @default.
- W2949672039 cites W2114096440 @default.
- W2949672039 cites W2120918097 @default.
- W2949672039 cites W2121083656 @default.
- W2949672039 cites W2121094803 @default.
- W2949672039 cites W2121158677 @default.
- W2949672039 cites W2141858379 @default.
- W2949672039 cites W2144999008 @default.
- W2949672039 cites W2149612795 @default.
- W2949672039 cites W2159270074 @default.
- W2949672039 cites W2161212572 @default.
- W2949672039 cites W2167380395 @default.
- W2949672039 cites W2170016329 @default.
- W2949672039 cites W2267962174 @default.
- W2949672039 cites W2287068230 @default.
- W2949672039 cites W2338485081 @default.
- W2949672039 cites W2401568148 @default.
- W2949672039 cites W2411751930 @default.
- W2949672039 cites W2543542605 @default.
- W2949672039 cites W2555146308 @default.
- W2949672039 cites W2567008309 @default.
- W2949672039 cites W2584813526 @default.
- W2949672039 cites W2597274540 @default.
- W2949672039 cites W2599773520 @default.
- W2949672039 cites W2607008467 @default.
- W2949672039 cites W2610160407 @default.
- W2949672039 cites W2612434226 @default.
- W2949672039 cites W2614144400 @default.
- W2949672039 cites W2731989040 @default.
- W2949672039 cites W2747643030 @default.
- W2949672039 cites W2757387674 @default.
- W2949672039 cites W2761461280 @default.
- W2949672039 cites W2775505403 @default.
- W2949672039 cites W2782774915 @default.
- W2949672039 cites W2788376770 @default.