Matches in SemOpenAlex for { <https://semopenalex.org/work/W2949741906> ?p ?o ?g. }
- W2949741906 endingPage "330" @default.
- W2949741906 startingPage "319" @default.
- W2949741906 abstract "We have used high-resolution orientation and distance measurements derived from electron paramagnetic resonance of a bifunctional spin label (BSL) to build and refine atomistic models of protein structure. We demonstrate this approach by investigating the effects of nucleotide binding on the structure of myosin's catalytic domain while myosin is in complex with actin. Constraints for orientation of individual helices were obtained in a previous study from continuous-wave electron paramagnetic resonance of myosin labeled at specific sites with BSLs in oriented muscle fibers. In this study, new distance constraints were derived from double electron-electron resonance on myosin constructs labeled with a BSL specifically at two sites. Using these complementary constraints together, we thoroughly characterize the BSL's rigid, highly stereoselective attachment to protein α-helices, which permits accurate measurements of orientation and distance. We also leverage these measurements to derive a novel, to our knowledge, structural model for myosin-II in complex with actin and MgADP and compare our model to other recent actomyosin structures. The described approach is applicable to any orientable complex (e.g., membranes or filaments) in which site-specific di-Cys mutation is feasible." @default.
- W2949741906 created "2019-06-27" @default.
- W2949741906 creator A5019616625 @default.
- W2949741906 creator A5022975491 @default.
- W2949741906 creator A5063616345 @default.
- W2949741906 date "2019-07-01" @default.
- W2949741906 modified "2023-10-16" @default.
- W2949741906 title "Atomistic Models from Orientation and Distance Constraints Using EPR of a Bifunctional Spin Label" @default.
- W2949741906 cites W1479573389 @default.
- W2949741906 cites W1499343594 @default.
- W2949741906 cites W1533349405 @default.
- W2949741906 cites W1598053850 @default.
- W2949741906 cites W1821679912 @default.
- W2949741906 cites W1862864832 @default.
- W2949741906 cites W1968266458 @default.
- W2949741906 cites W1977609596 @default.
- W2949741906 cites W1981954173 @default.
- W2949741906 cites W1983889563 @default.
- W2949741906 cites W1987474460 @default.
- W2949741906 cites W1988093831 @default.
- W2949741906 cites W1990561548 @default.
- W2949741906 cites W1997910573 @default.
- W2949741906 cites W2010695307 @default.
- W2949741906 cites W2015223366 @default.
- W2949741906 cites W2022378428 @default.
- W2949741906 cites W2025346903 @default.
- W2949741906 cites W2026145805 @default.
- W2949741906 cites W2029667189 @default.
- W2949741906 cites W2038862514 @default.
- W2949741906 cites W2039241916 @default.
- W2949741906 cites W2040899301 @default.
- W2949741906 cites W2041625063 @default.
- W2949741906 cites W2042651221 @default.
- W2949741906 cites W2043664506 @default.
- W2949741906 cites W2044629144 @default.
- W2949741906 cites W2046167970 @default.
- W2949741906 cites W2056126751 @default.
- W2949741906 cites W2056148801 @default.
- W2949741906 cites W2058371000 @default.
- W2949741906 cites W2069953906 @default.
- W2949741906 cites W2072529314 @default.
- W2949741906 cites W2085239860 @default.
- W2949741906 cites W2098513990 @default.
- W2949741906 cites W2099127313 @default.
- W2949741906 cites W2100954617 @default.
- W2949741906 cites W2101463683 @default.
- W2949741906 cites W2102997141 @default.
- W2949741906 cites W2116675254 @default.
- W2949741906 cites W2118200955 @default.
- W2949741906 cites W2120259237 @default.
- W2949741906 cites W2124026197 @default.
- W2949741906 cites W2129434797 @default.
- W2949741906 cites W2130955683 @default.
- W2949741906 cites W2131830655 @default.
- W2949741906 cites W2143205915 @default.
- W2949741906 cites W2144321597 @default.
- W2949741906 cites W2149298576 @default.
- W2949741906 cites W2149525061 @default.
- W2949741906 cites W2152301430 @default.
- W2949741906 cites W2152307221 @default.
- W2949741906 cites W2154663686 @default.
- W2949741906 cites W2164676813 @default.
- W2949741906 cites W2171082071 @default.
- W2949741906 cites W2194535754 @default.
- W2949741906 cites W2243195272 @default.
- W2949741906 cites W2273975555 @default.
- W2949741906 cites W2315787811 @default.
- W2949741906 cites W2416915225 @default.
- W2949741906 cites W2438805704 @default.
- W2949741906 cites W2474486403 @default.
- W2949741906 cites W2497318585 @default.
- W2949741906 cites W2784890560 @default.
- W2949741906 cites W2949351159 @default.
- W2949741906 cites W2949913660 @default.
- W2949741906 cites W336760737 @default.
- W2949741906 cites W4255555697 @default.
- W2949741906 doi "https://doi.org/10.1016/j.bpj.2019.04.042" @default.
- W2949741906 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6702148" @default.
- W2949741906 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/31301803" @default.
- W2949741906 hasPublicationYear "2019" @default.
- W2949741906 type Work @default.
- W2949741906 sameAs 2949741906 @default.
- W2949741906 citedByCount "0" @default.
- W2949741906 crossrefType "journal-article" @default.
- W2949741906 hasAuthorship W2949741906A5019616625 @default.
- W2949741906 hasAuthorship W2949741906A5022975491 @default.
- W2949741906 hasAuthorship W2949741906A5063616345 @default.
- W2949741906 hasBestOaLocation W29497419061 @default.
- W2949741906 hasConcept C121332964 @default.
- W2949741906 hasConcept C12554922 @default.
- W2949741906 hasConcept C125705527 @default.
- W2949741906 hasConcept C126838900 @default.
- W2949741906 hasConcept C143409427 @default.
- W2949741906 hasConcept C161790260 @default.
- W2949741906 hasConcept C16345878 @default.
- W2949741906 hasConcept C177462420 @default.
- W2949741906 hasConcept C185592680 @default.
- W2949741906 hasConcept C187961010 @default.