Matches in SemOpenAlex for { <https://semopenalex.org/work/W2949758238> ?p ?o ?g. }
- W2949758238 abstract "Stable kinetochore-microtubule attachment is essential for cell division. It requires recruitment of outer kinetochore microtubule binders by centromere proteins C and T (CENP-C and CENP-T). To study the molecular requirements of kinetochore formation, we reconstituted the binding of the MIS12 and NDC80 outer kinetochore subcomplexes to CENP-C and CENP-T. Whereas CENP-C recruits a single MIS12:NDC80 complex, we show here that CENP-T binds one MIS12:NDC80 and two NDC80 complexes upon phosphorylation by the mitotic CDK1:Cyclin B complex at three distinct CENP-T sites. Visualization of reconstituted complexes by electron microscopy supports this model. Binding of CENP-C and CENP-T to MIS12 is competitive, and therefore CENP-C and CENP-T act in parallel to recruit two MIS12 and up to four NDC80 complexes. Our observations provide a molecular explanation for the stoichiometry of kinetochore components and its cell cycle regulation, and highlight how outer kinetochore modules bridge distances of well over 100 nm." @default.
- W2949758238 created "2019-06-27" @default.
- W2949758238 creator A5011256990 @default.
- W2949758238 creator A5032442106 @default.
- W2949758238 creator A5058785617 @default.
- W2949758238 creator A5060454927 @default.
- W2949758238 creator A5066127029 @default.
- W2949758238 creator A5067807223 @default.
- W2949758238 creator A5071625312 @default.
- W2949758238 creator A5073374028 @default.
- W2949758238 date "2016-08-25" @default.
- W2949758238 modified "2023-09-24" @default.
- W2949758238 title "Molecular basis of outer kinetochore assembly on CENP-T" @default.
- W2949758238 cites W1940119905 @default.
- W2949758238 cites W1964523091 @default.
- W2949758238 cites W1967249732 @default.
- W2949758238 cites W1967696278 @default.
- W2949758238 cites W1975317507 @default.
- W2949758238 cites W1975648894 @default.
- W2949758238 cites W1978334390 @default.
- W2949758238 cites W1984128119 @default.
- W2949758238 cites W1986656413 @default.
- W2949758238 cites W1990300808 @default.
- W2949758238 cites W1996836461 @default.
- W2949758238 cites W2002697137 @default.
- W2949758238 cites W2005490271 @default.
- W2949758238 cites W2010280142 @default.
- W2949758238 cites W2017914102 @default.
- W2949758238 cites W2022046477 @default.
- W2949758238 cites W2024133477 @default.
- W2949758238 cites W2026920658 @default.
- W2949758238 cites W2027329338 @default.
- W2949758238 cites W2031398163 @default.
- W2949758238 cites W2034663072 @default.
- W2949758238 cites W2070575428 @default.
- W2949758238 cites W2071364502 @default.
- W2949758238 cites W2075763595 @default.
- W2949758238 cites W2076095878 @default.
- W2949758238 cites W2079741946 @default.
- W2949758238 cites W2083474696 @default.
- W2949758238 cites W2086283062 @default.
- W2949758238 cites W2099686283 @default.
- W2949758238 cites W2100455255 @default.
- W2949758238 cites W2100916752 @default.
- W2949758238 cites W2105793503 @default.
- W2949758238 cites W2112242180 @default.
- W2949758238 cites W2122956708 @default.
- W2949758238 cites W2123253626 @default.
- W2949758238 cites W2128918653 @default.
- W2949758238 cites W2133435867 @default.
- W2949758238 cites W2136801770 @default.
- W2949758238 cites W2147255166 @default.
- W2949758238 cites W2147290892 @default.
- W2949758238 cites W2147909090 @default.
- W2949758238 cites W2154093345 @default.
- W2949758238 cites W2167279371 @default.
- W2949758238 cites W2168277561 @default.
- W2949758238 cites W2172056867 @default.
- W2949758238 cites W2201676626 @default.
- W2949758238 cites W2209738096 @default.
- W2949758238 cites W2336779257 @default.
- W2949758238 cites W2344664973 @default.
- W2949758238 cites W4229736484 @default.
- W2949758238 doi "https://doi.org/10.1101/071613" @default.
- W2949758238 hasPublicationYear "2016" @default.
- W2949758238 type Work @default.
- W2949758238 sameAs 2949758238 @default.
- W2949758238 citedByCount "0" @default.
- W2949758238 crossrefType "posted-content" @default.
- W2949758238 hasAuthorship W2949758238A5011256990 @default.
- W2949758238 hasAuthorship W2949758238A5032442106 @default.
- W2949758238 hasAuthorship W2949758238A5058785617 @default.
- W2949758238 hasAuthorship W2949758238A5060454927 @default.
- W2949758238 hasAuthorship W2949758238A5066127029 @default.
- W2949758238 hasAuthorship W2949758238A5067807223 @default.
- W2949758238 hasAuthorship W2949758238A5071625312 @default.
- W2949758238 hasAuthorship W2949758238A5073374028 @default.
- W2949758238 hasBestOaLocation W29497582381 @default.
- W2949758238 hasConcept C104317684 @default.
- W2949758238 hasConcept C12554922 @default.
- W2949758238 hasConcept C185592680 @default.
- W2949758238 hasConcept C192838845 @default.
- W2949758238 hasConcept C20418707 @default.
- W2949758238 hasConcept C30481170 @default.
- W2949758238 hasConcept C54355233 @default.
- W2949758238 hasConcept C63491729 @default.
- W2949758238 hasConcept C86803240 @default.
- W2949758238 hasConcept C93304396 @default.
- W2949758238 hasConcept C95444343 @default.
- W2949758238 hasConceptScore W2949758238C104317684 @default.
- W2949758238 hasConceptScore W2949758238C12554922 @default.
- W2949758238 hasConceptScore W2949758238C185592680 @default.
- W2949758238 hasConceptScore W2949758238C192838845 @default.
- W2949758238 hasConceptScore W2949758238C20418707 @default.
- W2949758238 hasConceptScore W2949758238C30481170 @default.
- W2949758238 hasConceptScore W2949758238C54355233 @default.
- W2949758238 hasConceptScore W2949758238C63491729 @default.
- W2949758238 hasConceptScore W2949758238C86803240 @default.
- W2949758238 hasConceptScore W2949758238C93304396 @default.
- W2949758238 hasConceptScore W2949758238C95444343 @default.