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• W2949762801 abstract "Abstract Recent developments in phase contrast microscopy have enabled the label‐free visualization of certain organelles due to their distinct morphological features, making this method an attractive alternative in the study of cellular dynamics. However tubular structures such as endoplasmic reticulum (ER) networks and complex dynamics such as the fusion and fission of mitochondria, due to their low phase contrast, still need fluorescent labeling to be adequately imaged. In this article, we report a quantitative phase microscope with ultra‐oblique illumination that enables us to see those structures and their dynamics with high contrast for the first time without labeling. The imaging capability was validated through comparison to the fluorescence images with the same field‐of‐view. The high image resolution (~270 nm) was validated using both beads and cellular structures. Furthermore, we were able to record the vibration of ER networks at a frame rate of 250 Hz. We additionally show complex cellular processes such as remodeling of the mitochondria networks through fusion and fission and vesicle transportation along the ER without labels. Our high spatial and temporal resolution allowed us to observe mitochondria “spinning”, which has not been reported before, further demonstrating the advantages of the proposed method." @default.
• W2949762801 created "2019-06-27" @default.
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• W2949762801 date "2019-06-30" @default.
• W2949762801 modified "2023-10-11" @default.
• W2949762801 title "Quantitative phase microscopy with enhanced contrast and improved resolution through ultra‐oblique illumination (UO‐QPM)" @default.
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• W2949762801 doi "https://doi.org/10.1002/jbio.201900011" @default.
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