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- W2949805591 abstract ": Pif1 family helicases represent a highly conserved class of enzymes involved in multiple aspects of genome maintenance. Many Pif1 helicases are multi-domain proteins, but the functions of their non-helicase domains are poorly understood. Here, we characterized how the N-terminal domain (NTD) of the Saccharomyces cerevisiae Pif1 helicase affects its functions both in vivo and in vitro. Removal of the Pif1 NTD alleviated the toxicity associated with Pif1 overexpression in yeast. Biochemically, the N-terminally truncated Pif1 (Pif1ΔN) retained in vitro DNA binding, DNA unwinding, and telomerase regulation activities, but these activities differed markedly from those displayed by full-length recombinant Pif1. However, Pif1ΔN was still able to synergize with the Hrq1 helicase to inhibit telomerase activity in vitro, similar to full-length Pif1. These data impact our understanding of Pif1 helicase evolution and the roles of these enzymes in the maintenance of genome integrity." @default.
- W2949805591 created "2019-06-27" @default.
- W2949805591 creator A5015593712 @default.
- W2949805591 creator A5028783197 @default.
- W2949805591 creator A5061031479 @default.
- W2949805591 date "2019-05-28" @default.
- W2949805591 modified "2023-09-25" @default.
- W2949805591 title "The Biochemical Activities of the Saccharomyces cerevisiae Pif1 Helicase Are Regulated by Its N-Terminal Domain" @default.
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- W2949805591 doi "https://doi.org/10.3390/genes10060411" @default.
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