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• W2949846615 startingPage "1081" @default.
• W2949846615 abstract "Abstract We developed an efficient multi-enzyme cascade reaction to produce (R)- or (S)-3,4-Dihydroxyphenyllactic acid [(R)- or (S)-Danshensu, (R)- or (S)-DSS] from 3,4-Dihydroxyphenyl-l-alanine (l-DOPA) in Escherichia coli by introducing tyrosine aminotransferase (tyrB), glutamate dehydrogenase (cdgdh) and d-aromatic lactate dehydrogenase (csldhD) or l-aromatic lactate dehydrogenase (tcldhL). First, the genes in the pathway were overexpressed and fine-tuned for (R)- or (S)-DSS production. The resulting strain, E. coli TGL 2.1 and E. coli TGL 2.2, which overexpressed tyrB with the stronger T7 promoter and cdgdh, cs ldhD or tcldhL with the weaker Trc promoter, E. coli TGL 2.1 yielded 57% increase in (R)-DSS production: 59.8 ± 2.9 mM. Meanwhile, E. coli TGL 2.2 yielded 54% increase in (S)-DSS production: 52.2 ± 2.4 mM. The optimal concentration of L-glutamate was found to be 20 mM for production of (R)- or (S)-DSS. Finally, l-DOPA were transformed into (R)- or (S)-DSS with an excellent enantiopure form (enantiomeric excess &gt; 99.99%) and productivity of 6.61 mM/h and 4.48 mM/h, respectively." @default.
• W2949846615 created "2019-06-27" @default.
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• W2949846615 date "2019-08-01" @default.
• W2949846615 modified "2023-09-26" @default.
• W2949846615 title "Redox self-sufficient biocatalyst system for conversion of 3,4-Dihydroxyphenyl-<scp>l</scp>-alanine into (<i>R</i>)- or (<i>S</i>)-3,4-Dihydroxyphenyllactic acid" @default.
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• W2949846615 doi "https://doi.org/10.1007/s10295-019-02200-7" @default.
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• W2949846615 hasPublicationYear "2019" @default.
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