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- W2949937090 abstract "Recently, research has focused on bone marrow derived multipotentmesenchymal precursor cells (MPC) for their potential clinical use in boneengineering. Prior to clinical application, MPC-based treatment concepts need to beevaluated in preclinical, immunocompetent, large animal models. Sheep in particularare considered a valid model for orthopaedic and trauma related research. However,ovine MPC and their osteogenic potential remain poorly characterized. In the presentstudy, ex vivo expanded MPC isolated from ovine bone marrow proliferated at ahigher rate than osteoblasts (OB) derived from tibial compact bone as assessedusing standard 2D culture. MPC expressed the respective phenotypic profile typicalfor different mesenchymal cell populations (CD14-/CD31-/CD45-/CD29+/CD44+/CD166+) and showed a multilineage differentiation potential. Whencompared to OB, MPC had a higher mineralization potential under standardosteogenic culture conditions and expressed typical markers such as osteocalcin,osteonectin and type I collagen at the mRNA and protein level. After 4 weeks in 3Dculture, MPC constructs demonstrated higher cell density and mineralization, whilstcell viability on the scaffolds was assessed >90%. Cells displayed a spindle-likemorphology and formed an interconnected network. Implanted subcutaneously intoNOD/SCID mice on type I collagen coated polycaprolactone-tricalciumphosphate(mPCL-TCP) scaffolds, MPC presented a higher developmental potential thanosteoblasts. In summary, this study provides a detailed in vitro characterisation ofovine MPC from a bone engineering perspective and suggests that MPC providepromising means for future bone disease related treatment applications." @default.
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- W2949937090 date "2010-10-01" @default.
- W2949937090 modified "2023-09-28" @default.
- W2949937090 title "Ovine bone and marrow derived progenitor cells : isolation, characterization, and osteogenic potential" @default.
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